John A. Mangos

Isolated Parotid Acinar Cells from Patients with Cystic Fibrosis. Morphology and Composition

Studies of the morphology and composition of isolated parotid acinar cells from patients with cystic fibrosis were conducted. It was demonstrated that these cells are larger than cells from controls and have higher concentrations of amylase, Ca, and Na and lower concentrations of K which is suggestive of functional abnormalities in homeostatic and secretory processes.

Micropuncture study of postnatal functional maturation of the rat parotid.

The postnatal function maturation of the rat parotid was investigated using micropuncture and microanalytical techniques. The cationic composition of the luminal fluid at different levels of the duct system, the net transductal fluxes of Na, K, and H2O and the salivary excretion of protein and amylase in immature and mature rats were determined.

Isolated Parotid Acinar Cells from Patients With Cystic Fibrosis. Functional Characterization

Studies of the homeostasis and secretory function of isolated parotid acinar cells from patients with cystic fibrosis were conducted. It was demonstrated that the transmembrane fluxes of Na and the muscarinic cholinergic receptor stimulus-secretion coupling were depressed, while the stimulus-secretion coupling of the beta-adrenergic receptor was hyper-reactive.

Characterization of Autonomic Receptors in Isolated Human Parotid Acinar Cells

Isolated human parotid acinar cells have been used for the in vitro characterization of the muscarinic cholinergic and alpha- and beta-adrenergic receptors of these cells. The agonist-antagonist interactions at the receptor level were studied, and the role of the receptor-activated cellular systems in the process of secretion was characterized.

Role of cGMP in Isolated Rat Parotid Acinar Cells

The role of cGMP as the intracellular mediator of the activation of cholinergic receptors in the secretory cells of the rat parotid gland was investigated in vitro using isolated rat parotid acinar cells. It was demonstrated that this cyclic nucleotide is involved in the intracellular translation of activation of muscarinic cholinergic receptors in these cells.

Secretion of Monovalent Ions and Water in Ferret Salivary Glands: A Micropuncture Study

The acinar mechanisms of secretion of the monovalent ions, Na+, K+, Cl-, and HCO- 3, and water in the parotid and submandibular glands of the ferret, Mustela putorius furo, were investigated using micropuncture and microanalytical techniques during salivation induced by pilocarpine. The osmolarity and monovalent ionic composition of the primary secretory fluid obtained from the intercalated ducts of these glands were determined.

Fluid fluxes in the ferret trachea

A net reabsorption of fluid was observed in isolated ferret trachea under control conditions. Cholinergic stimulation resulted in net secretion of fluid while atropine blocked this response without any effect on the basal process of fluid reabsorption.

A Micropuncture Study of the Handling of Calcium by the Rat Parotid

The handling of Ca by the rat parotid gland was investigated using micropuncture, microperfusion, and microanalytical techniques. Concentrations of Ca were measured in salivary fluid from intercalated, lobular and main ducts, and net transductal fluxes of Ca and water were calculated during stimulation of secretion with acetylcholine, pilocarpine, and DLisoproterenol.

Morphological and Functional Characterization of Isolated Human Parotid Acinar Cells

A method for the isolation of human parotid acinar cells using enzymatic dispersion and mild mechanical forces has been developed. The isolated acinar cells are morphologically and functionally intact, thus suitable for studies of the cellular physiology of secretion.

Isolated Parotid Acinar Cells from DL-Isoproterenol-treated Rats. A Cellular Model for Cystic Fibrosis

The morphology and composition of isolated acinar cells from the enlarged parotid glands of rats treated chronically with DL-isoproterenol were investigated and compared with isolated acinar cells from parotids of control untreated rats. Changes similar to those observed in parotid acinar cells from patients with cystic fibrosis were demonstrated.