John A. Udobang

Antiplasmodial and antiulcer activities of Melanthera scadens.

OBJECTIVE To evaluate the antimalarial and antiulcerogenic activities of leaf extract and fractions of Melanthera scandens (M. scandens). METHODS The crude leaf extract (37-111 mg/kg) and fractions (chloroform, ethylacetate and methanol; 78 mg/kg) of M. scadens were investigated for antiplasmodial activity against chloroquine-sensitive Plasmodium berghei infections in mice and for antiulcer activity against experimentally-induced ulcers. The antimalarial activity during early and established infections as well as prophylactic was investigated. Artesunate (5 mg/kg) and pyrimethamine (1.2 mg/kg) were used as positive controls. Thin films made from tail blood of each mouse were used to assess the level of parasitaemia of the mice. Antiulcer activity of the crude extract was also evaluated against indomethacin, ethanol and histamine induced ulcers. RESULTS The extract and its fractions dose-dependently reduced parasitaemia induced by chloroquine-sensitive Plasmodium berghei infection in prophylactic, suppressive and curative models in mice. These reductions were statistically significant (P<0.001). They also improved the mean survival time (MST) from 9.28 to 17.73 days as compared with the control (P<0.01-0.001). The activities of extract/fractions were incomparable to that of the standard drugs i.e. artesunate and pyrimethamine. On experimentally-induced ulcers, the extract inhibited indomethacin, ethanol and histamine induced ulcers. These inhibitions were statistically significant (P<0.001) and in a dose-dependent fashion. CONCLUSIONS The antiplasmodial and antiulcerogenic effects of this plant may in part be mediated through the chemical constituents of the plant.

Assessing the psychological well-being of caregivers of people living with HIV/AIDS in Niger Delta region, Nigeria

Abstract Despite the availability of anti-retroviral drugs and treatment, the care and support of people living with HIV/AIDS has continued to be a major challenge, with greater impact not only on the people living the disease. The burden associated with care often results in high level of stress among caregivers, usually manifesting as anxiety and/or depression. This study assesses the levels of stress among caregivers of people living with HIV/AIDS in Niger Delta region of Nigeria, in order to determine their coping strategies. Between July and December 2008, 322 caregivers of people living with HIV/AIDS attending HIV clinic of the University of Uyo Teaching Hospital were randomly assessed for stress (anxiety and depression), using Zungs Self-Rating Depression Scale (SDS) and Self-Report Questionnaire (SRQ-20). A total of 293 caregivers comprising 98 (33.4%) males and 195 (66.6%) females were analysed. Mean age of males was 44.0±4.5 years and females was 39.3±4.0 years. The difference in the mean was statistically significant (p<0.001). Of the 293 caregivers, 191 (65.2%) and 115 (39.2%) were scored high on SDS and SRQ-20, respectively (representing the levels of stress). Using SDS groups on SRQ-20 subscales, 134 (45.7%) had anxiety only, 23 (7.8%) depression only, 52 (17.7%) had a mixture of anxiety and depression, while 11 (3.7%) exhibited suicidal tendencies. There is increasing level of stress in caregiving. Therefore, adequate attention must be given to the psychological well-being of caregivers to enable them contribute positively to the care of people living with HIV/AIDS.

Analgesic and antimalarial activities of crude leaf extract and fractions of Acalypha wilkensiana.

AIM OF THE STUDY Antiplasmodial and analgesic activities of leaf extract and fractions of Acalypha wilkensiana were evaluated to ascertain the folkloric claim of its antimalarial and analgesic activities. MATERIALS AND METHODS The crude leaf extract (220-659 mg/kg) and fractions (chloroform and aqueous; 440 mg/kg) of Acalypha wilkensiana were investigated for antiplasmodial activity against chloroquine sensitive Plasmodium berghei infections in mice and for analgesic activity against chemical and heat-induced pains. The antiplasmodial activity during early and established infections as well as prophylactic activity were investigated. Chloroquine (5mg/kg) and pyrimethamine (1.2mg/kg) were used as positive controls. Thin films made from tail blood of each mouse were used to assess the level of parasitaemia of the mice. Analgesic activity of the crude extract was also evaluated against acetic acid, formalin and heat-induced pains. RESULTS The extract and its fractions dose-dependently reduced parasitaemia induced by chloroquine sensitive Plasmodium berghei infection in prophylactic, suppressive and curative models in mice. These reductions were statistically significant (p<0.001). They also improved the mean survival time (MST) from 16 to 22 days relative to control (p<0.01-0.001). The activities of extract/fractions were incomparable to that of the standard drugs used (chloroquine and pyrimethamine). On chemically and thermally induced pains, the extract inhibited acetic acid and formalin-induced inflammation as well as hot plate-induced pain in mice. These inhibitions were statistically significant (p<0.001) and in a dose-dependent fashion. CONCLUSION The antiparasitaemic and analgesic effects may in part be mediated through the chemical constituents of the plant.

Antidiabetic activities of ethanolic extract and fraction of Anthocleista djalonensis

OBJECTIVE To evaluate the antidiabetic activities of ethanolic root extract/fraction of Anthocleista djalonensis (A. djalonensis) in alloxan-induced diabetic rats. METHODS A. djalonensis root extract/fractions (37-111 mg/kg) were administered to alloxan-induced diabetic rats for 14 days and blood glucose levels (BGLs) of the diabetic rats were monitored at intervals of hours and days throughout the duration of the treatment. RESULTS Treatment of alloxan-induced diabetic rats with the extract/fractions caused a significant (P<0.001) reduction in fasting BGLs of the diabetic rats both in acute study and prolonged treatment (2 weeks). The activities of the extract and fractions were more than that of the reference drug, glibenclamide. CONCLUSIONS These results suggest that the root extract/fractions of A. djalonensis possess antidiabetic effect on alloxan-induced diabetic rats and this justifies its use in ethnomedicine and can be exploited in the management of diabetes.

Antiplasmodial and analgesic activities of Clausena anisata.

OBJECTIVE Antiplasmodial and analgesic activities of the leaf extract and fractions of Clausena anisata (C. anisata) were evaluated for antimalarial and analgesic activities. METHODS The crude leaf extract (39-117 mg/kg) and fractions (chloroform and acqeous; 78 mg/kg) of C. anisata were investigated for antiplasmodial activity against chloroquine-sensitive Plasmodium berghei (P. berghei) infections in mice using suppressive, prophylactic and curative models and analgesic activity against acetic acid, formalin and heat-induced pains. Artesunate, 5 mg/kg and pyrimethamine, 1.2 mg/kg were used as positive controls. Thin films made from tail blood of each mouse were used to assess the level of parasitaemia of the mice. RESULTS The extract and its fractions dose-dependently reduced parasitaemia induced by chloroquine-sensitive P. berghei in prophylactic, suppressive and curative models in mice. These reductions were statistically significant (P<0.001). They also improved the mean survival time (MST) from 17 to 21 days relative to control (P<0.01 - 0.001). On chemically and thermally-induced pains, the extract inhibited acetic acid and formalin-induced inflammation as well as hot plate-induced pain in mice. These inhibitions were statistically significant (P<0.001) and in a dose-dependent fashion. CONCLUSIONS The antiplasmodial and analgesic effects of this plant may in part be mediated through its chemical constituents and it can be concluded that the C. anisata possess significant antimalarial and analgesic properties.