John B. Miale

EFFECT OF MICROWAVE RADIATION ON THE HEMOPOIETIC SYSTEM OF THE RAT.

Abstract Exposure of Osborne-Mendel, CFN, and Fischer strain rats to minimal doses of microwave radiation (24,000 megacycles) induced a significant leucocytosis, lymphocytosis, and neutrophilia after 7 hours of continuous exposure to 20 mw/cm2 with recovery in 1 week; 10 minutes of continuous exposure to 20 mw/cm2; and 3 hours of continuous exposure to 10 mw/cm2 with recovery in 2 days. Effects on erythrocytes, hemoglobin, and hematocrit differed in the three strains used. In Osborne-Mendel and CFN rats, all values increased; in Fischer rats, they decreased.

The effects of oral contraceptives on the results of laboratory tests.

Abstract The literature on the effects of oral contraceptives on the results of clinical laboratory tests has been reviewed critically. One hundred tests are affected significantly. These have been tabulated and, for each, an estimate has been made as to whether the effect is slight, moderate, or severe.

Serum Haptoglobin in Rabbits after Subcutaneous Injection of Freund's Adjuvant or Turpentine.

Summary The concentration of serum haptoglobin in normal and stimulated rabbits was studied. Hyperhaptoglobinemia could be produced by both single and multiple subcutaneous injections of turpentine; multiple injections resulted in more striking and longer lasting hyperhaptoglobinemia. Single or multiple subcutaneous injections of Freunds adjuvant caused no significant elevation of the serum haptoglobin concentration.

Quantitation of human chorionic gonadotropin in urine using the slide immunologic test for pregnancy

Abstract 1. 1. The use of the slide immunologic test in a semiquantitative procedure for determining HCG titers in urine has been described. This method is simple, reliable, and rapid. The test can be performed in one hour. 2. 2. The sensitivity and accuracy of this method have been compared with those of the Friedman test and the results found have been good. 3. 3. The sensitivity of the immunologic test described is approximately 5 I.U. per milliliter, as determined by the use of the Second International Standard for human chorionic gonadotrophin. 4. 4. The geometric mean of titers observed in 295 urine samples obtained at varying times throughout pregnancy is presented. In the practical use of this test, a titer of 1:64 (320 I.U. per milliliter) is considered the high limit of normal during the first trimester of pregnancy. 5. 5. The potential use of this serologic test in the diagnosis of abnormal conditions like hydatidiform moles and chorionepitheliomas is discussed.

Hematologic effects of Crotalus adamanteus (rattlesnake) venom

Summary1.Crotalus adamanteus venom produces a striking neutrophilic leukocytosis in dogs whether administered intramuscularly or intravenously.2.Intramuscular administration of a lethal dose (1.28 mg/kg) of crystallineCrotalus adamanteus venom to dogs also produced: a terminal increase in serum nonprotein nitrogen, a hypoproteinemia, a reduction in plasma fibrinogen, and a terminal shortening of coagulation time.3.It is noteworthy that no effects of this venom were noted on the serum bilirubin concentration. It follows, therefore, that contrary to existing impressions this venom does not produce any significant degree of hemolysis in vivo.4.In addition, the intramuscular administration ofCrotalus adamanteus venom was found not to significantly affect the: blood glucose concentration, the platelet count, the plasma prothrombin time, the prothrombin time withRussells viper venom, the prothrombin consumption, the thromboplastin generation, or the fibrinolysin activity. Two of five dogs showed a transient rise in erythrocyte count and hematocrit.5.By contrast,Naja flava venom did not produce neutrophilic leukocytosis when administered in a subacutely toxic dose (0.2 mg/kg/day).6.Intravenously administeredCrotalus adamanteus venom did not counteract the neutropenic effects of orally administered Myleran in dogs.

Effect of banana ingestion on urinary excretion of 5-hydroxyindole compounds in normal adults.

Summary 1. Banana ingestion (12 g/kg/24 hr) by normal adults produced an average 2-fold increase in urinary excretion of 5-OH indoles. This was not of such magnitude as to suggest an erroneous diagnosis of malignant carcinoid. 2. Collection of 24-hour urine specimens and use of a quantitative test are advocated over collection of random urine specimens. Positive screening tests should be checked by a quantitative method.

A synthetic iodinated polypeptide that inhibits polymerization of fibrinogen.

SummarySynthetic iodopeptides exert a powerful anticoagulant activity. Data are presented that indicate the anticoagulant effect to be an inhibition of fibrin polymerization.ZusammenfassungGewisse synthetische Polypeptide geben nach Jodierung stark koagulations-hemmende Derivate. Diese Wirkung scheint nach den bisherigen Befunden auf einer Hemmung der Fibrinopolymerisation zu beruhen.

Clinical Approach to the Patient With a Hemorrhagic Disorder

Diagnosis of hemorrhagic disorders depends heavily on results of laboratory examinations; however, a sound and thorough clinical approach is also important. The proper clinical approach should first reveal whether or not an abnormality in hemostasis exists. This conclusion would lead to proper investigations, which are determined by consultation between clinician and pathologist. The results of these investigations would guide rational therapy.