John Hamilton Kennedy

Sensitive Sandwich Enzyme Immunoassay for Serum Ferritin on Microtitre Plates

A solid-phase sandwich enzyme immunoassay for serum ferritin is described. The assay procedure was simple, showed good precision, and was suitable for the routine determination of serum ferritin concentration in a clinical laboratory. The assay compared well with a conventional radioimmunoassay for ferritin. The assay was extremely sensitive and had a detection limit of 2·2 pg.

The application of thin-layer gel filtration chromatography in the rapid assessment of protein-protein coupling reactions

The use of enzymes as markers when covalently coupled to various antigens or antibodies has wide application in medical science. A variety of bifunctional reagents has been used to produced the conjugates, and there have been some attempts to investigate the conditions of reaction necessary to obtain good coupling, whilst preserving the biological function of the molecules. The present study describes the influence of coupling conditions on the coupling of a alpha-amylase and albumin by a range of commercially available bifunctional coupling reagents, and the application of thin-layer gel filtration chromatography for the rapid qualitative/semi-quantitative assessment of coupling reactions.