John M. Gaska

Efficacy of Fungicides on Sclerotinia sclerotiorum and Their Potential for Control of Sclerotinia Stem Rot on Soybean

Sclerotinia stem rot of soybean, caused by Sclerotinia sclerotiorum, is a major disease in the north central region of the United States. One approach to managing Sclerotinia stem rot on soybean is the use of fungicides. S. sclerotiorum was assayed for sensitivity to benomyl, tebuconazole, thiophanate methyl, and vinclozolin in pure cultures on agar medium, inoculated soybean seedlings, detached inoculated leaves, and in experimental field plots. To evaluate the inhibitory effect of four fungicides on growth of S. sclerotiorum in vitro, potato dextrose agar (PDA) was amended with the fungicides at six concentrations. Based on measurements of fungal radial growth, vinclozolin was the most effective in inhibiting S. sclerotiorum mycelial growth at 1.0 μg a.i./ml of PDA. Ranges of reduction of radial growth of 91 isolates of S. sclerotiorum on PDA amended with thiophanate methyl and vinclozolin were 18 to 93% and 93 to 99%, respectively, when compared with the nonamended agar control. Benomyl, thiophanate methyl, and vinclozolin applied to greenhouse-grown seedlings prevented S. sclerotiorum from expressing symptoms or signs on leaf tissue. Detached leaves sprayed with thiophanate methyl and then inoculated with mycelial plugs of S. sclerotiorum did not express symptoms or signs. Of 13 different environments in Illinois, Indiana, Ohio, and Wisconsin from 1995 through 2000, six had low Sclerotinia stem rot incidence (<1%), three environments had low to moderate Sclerotinia stem rot incidence (5 to 25%), and four environments had high Sclerotinia stem rot incidence (>25%). When disease incidence was high, no consistent control of Sclerotinia stem rot was observed with benomyl or thiophanate methyl using different application systems. However, under low disease incidence, spray systems that were able to penetrate the canopy reduced the incidence of Sclerotinia stem rot an average of 50%.

Seasonal Progression and Agronomic Impact of Tobacco streak virus on Soybean in Wisconsin

Pod necrosis and dieback of terminal portions of stems, commonly called bud blight, are observed in soybean fields and associated with Tobacco streak virus (TSV), an understudied virus of soybean. Furthermore, many TSV-infected plants are asymptomatic. The objectives of this study were to characterize the distribution and seasonal progress of TSV-infected plants in both natural and controlled epidemics, and the agronomic impact of TSV on soybean in plots with controlled introduction of inoculum. Incidence of TSV-positive samples ranged from 17 to 56% in a general survey. In the presence of natural sources of inoculum, the incidence of TSV-infected plants ranged from 10 to 95% depending on cultivar and location, and peaked at growth stage R2, but detection dropped dramatically at R5. During 2001, significant yield loss and incidence of mottled seed were associated with TSV, but results were confounded by a high incidence of SMV. In 2002, SMV was controlled by cultivar selection, and a 25% reduction in grain yield was attributable to TSV. The incidence of mottled seed and green stem syndrome were low in the presence of TSV. Reductions in plant density and final plant height contributed to reduced yields. However, no significant differences were found in seed number per plant and 100-seed weight. Data indicate that plant mortality was the main mechanism by which TSV caused yield loss in induced epidemics.

Effect of Location, Cultivar, and Diseases on Grain Yield of Soft Red Winter Wheat in Wisconsin

Knowledge is limited about the impact of foliar diseases on wheat yield in Wisconsin. The objective of this study was to compare yield and diseases of wheat cultivars in several locations in Wisconsin in 2009 and 2010. Thirty-six wheat cultivars were planted in a randomized complete block design at field sites near Arlington, Chilton, and Lancaster, WI. At a fourth location, Janesville, WI, the design was a split plot with foliar fungicide application at Zadoks growth stage (GS) 45 at the whole-plot level and cultivar at the subplot level. Disease assessments were made four times during the growing season for powdery mildew (PM), Septoria/Stagonospora leaf blotch (SLB), and leaf rust. Incidence and severity of Fusarium head blight were assessed on 100 heads per plot at GS 85. Linear mixed-model analyses were used to study the effects of location, cultivar, and disease on grain yield (α = 0.05). Overall, SLB and PM were the most prevalent diseases. SLB severity was uniform among locations and PM was most prevalent at Arlington and Chilton. In both years, yield was affected by location, cultivar, location-cultivar interaction, and location-SLB and location-PM interactions. Yield was also negatively affected by PM in 2010. No effect of fungicide on disease severity or yield was observed at Janesville in either year. These results suggest that cultivar selection and location strongly influence grain yield in Wisconsin and that powdery mildew is capable of reducing grain yield.