John Mccafferty
Medical Research Council
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Publication
Featured researches published by John Mccafferty.
Applied Biochemistry and Biotechnology | 1994
John Mccafferty; Kevin J. Fitzgerald; John C. Earnshaw; David John Chiswell; John R. Link; Rodger Smith; John H. Kenten
Functional antibody fragments may be displayed on the surface of filamentous bacteriophage by introducing variable region genes into the viral genome at a gene encoding a viral coat protein. “Phage display” enables the isolation of antibody genes from large libraries according to the binding specificities they encode. We have constructed a new phage-display vector encoding a polyhistidine tag that has been used for rapid purification of soluble antibody fragments. An antibody library derived from immunized mice was cloned into this vector. This library was panned against the transition state analog RT3, and a high proportion of binders isolated after two rounds of panning. PCR analysis revealed that there were 24 different pattern groups. Sequencing of 15 clones within the major pattern group revealed 10 related clones with a range of point mutations. Thus, phage display can provide a large diverse repertoire of candidate catalytic antibodies based on TSA selection and screening.
Nature | 1990
John Mccafferty; Andrew D. Griffiths; Greg Winter; David John Chiswell
Journal of Molecular Biology | 1991
James D. Marks; Hennie R. Hoogenboom; Timothy Peter Bonnert; John Mccafferty; Andrew D. Griffiths; Greg Winter
Archive | 1991
John Mccafferty; Anthony Richard Pope; Kevin Stuart Johnson; Hendricus Renerus Jacobus Mattheus Hoogenboom; Andrew D. Griffiths; Ronald Henry Jackson; Kaspar Philipp Holliger; James David Marks; Timothy Piers Clackson; David John Chiswell; Gregory Paul Winter; Timothy Peter Bonnert
Archive | 1992
Andrew David Griffiths; Hendricus Renerus Jacobus Mattheus Hoogenboom; James David Marks; John Mccafferty; Gregory Paul Winter; Geoffrey Walter Grigg
Archive | 2000
Andrew D. Griffiths; Hendricus Renerus Jacobus Mattheus Hoogenboom; James David Marks; John Mccafferty; Gregory Paul Winter; Geoffrey Walter Grigg
Protein Engineering | 1991
John Mccafferty; Ronald Henry Jackson; David John Chiswell
Protein Engineering | 1996
Helen L. Parsons; John C. Earnshaw; Jane Wilton; Kevin Stuart Johnson; Paula A. Schueler; Walt Mahoney; John Mccafferty
Phage Display of Peptides and Proteins#R##N#A Laboratory Manual | 1996
John Mccafferty; Kevin Stuart Johnson
Archive | 1994
Rodger Smith; John Mccafferty; David John Chiswell; Michael J. Darsley; Kevin J. Fitzgerald; John H. Kenten; Mark T. Martin; Richard C. Titmas; Richard O Williams