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Dive into the research topics where John Mccafferty is active.

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Featured researches published by John Mccafferty.


Applied Biochemistry and Biotechnology | 1994

Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display

John Mccafferty; Kevin J. Fitzgerald; John C. Earnshaw; David John Chiswell; John R. Link; Rodger Smith; John H. Kenten

Functional antibody fragments may be displayed on the surface of filamentous bacteriophage by introducing variable region genes into the viral genome at a gene encoding a viral coat protein. “Phage display” enables the isolation of antibody genes from large libraries according to the binding specificities they encode. We have constructed a new phage-display vector encoding a polyhistidine tag that has been used for rapid purification of soluble antibody fragments. An antibody library derived from immunized mice was cloned into this vector. This library was panned against the transition state analog RT3, and a high proportion of binders isolated after two rounds of panning. PCR analysis revealed that there were 24 different pattern groups. Sequencing of 15 clones within the major pattern group revealed 10 related clones with a range of point mutations. Thus, phage display can provide a large diverse repertoire of candidate catalytic antibodies based on TSA selection and screening.


Nature | 1990

Phage antibodies: filamentous phage displaying antibody variable domains

John Mccafferty; Andrew D. Griffiths; Greg Winter; David John Chiswell


Journal of Molecular Biology | 1991

By-passing immunization: Human antibodies from V-gene libraries displayed on phage

James D. Marks; Hennie R. Hoogenboom; Timothy Peter Bonnert; John Mccafferty; Andrew D. Griffiths; Greg Winter


Archive | 1991

Method for producing members of specific binding pairs

John Mccafferty; Anthony Richard Pope; Kevin Stuart Johnson; Hendricus Renerus Jacobus Mattheus Hoogenboom; Andrew D. Griffiths; Ronald Henry Jackson; Kaspar Philipp Holliger; James David Marks; Timothy Piers Clackson; David John Chiswell; Gregory Paul Winter; Timothy Peter Bonnert


Archive | 1992

Production of anti-self antibodies from antibody segment repertoires and displayed on phage

Andrew David Griffiths; Hendricus Renerus Jacobus Mattheus Hoogenboom; James David Marks; John Mccafferty; Gregory Paul Winter; Geoffrey Walter Grigg


Archive | 2000

Production of anti-self bodies from antibody segment repertories and displayed on phage

Andrew D. Griffiths; Hendricus Renerus Jacobus Mattheus Hoogenboom; James David Marks; John Mccafferty; Gregory Paul Winter; Geoffrey Walter Grigg


Protein Engineering | 1991

Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage

John Mccafferty; Ronald Henry Jackson; David John Chiswell


Protein Engineering | 1996

Directing phage selections towards specific epitopes

Helen L. Parsons; John C. Earnshaw; Jane Wilton; Kevin Stuart Johnson; Paula A. Schueler; Walt Mahoney; John Mccafferty


Phage Display of Peptides and Proteins#R##N#A Laboratory Manual | 1996

CHAPTER 6 – Construction and Screening of Antibody Display Libraries

John Mccafferty; Kevin Stuart Johnson


Archive | 1994

Isolation and production of catalytic antibodies using phage technology

Rodger Smith; John Mccafferty; David John Chiswell; Michael J. Darsley; Kevin J. Fitzgerald; John H. Kenten; Mark T. Martin; Richard C. Titmas; Richard O Williams

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Anthony Richard Pope

Wellcome Trust Sanger Institute

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