John W. Greene

Endomyocardial ultrastructural findings in preeclampsia

Ultrastructural findings in endomyocardial biopsy specimens obtained during cardiac catheterization in a patient with severe preeclampsia are described. Intramyocardial vessels revealed prominent swelling of the endothelial cell cytoplasm. In addition, cardiac myocyte mitochondria showed swelling and clearing within the matrix. Endomyocardial ultrastructural injury occurs in severe preeclampsia.

Correlation of estriol excretion patterns of pregnant women with subsequent development of their children

Abstract This paper is the first of a series concerning the follow-up of children born of pregnancies during which the maternal urinary estriol excretion has been studied. Four groups of children are presented: offspring of mothers with (1) normal urinary estriol excretion, (2) sometimes abnormally low estriol excretion but normal just before delivery, (3) declining urinary estriol, and (4) chronically low estriol. These 34 children were 1 to 7 years of age at follow-up. Growth parameters and developmental progress were measured. Developmental and intelligence tests used were the Denver Developmental Test, Slosson Intelligence Test, the Vineland Social Maturity Scale, and the Bender Gestalt Test of Visual Motor Perception. Results of these tests are shown in relation to the urinary estriol excretion patterns, the maternal pregnancy complications, infant neonatal course, and socioeconomic status. The study reveals that low estriol excretion before delivery is compatible with infant development and intelligence in the normal range.

Comparison of three methods for determination of urinary estriol during late pregnancy

Abstract Three colorimetric methods for determination of urinary estriol have been compared. When statistical analysis is applied to the estrogen measured by the three methods, one finds that in urine from a normal pregnancy the methods are comparable and show a consistent relationship. The Ittrich method may be used as a rapid screening technique. The short procedure is rapid and gives slightly higher values than the long procedure. In the presence of glycosuria there tends to be greater variation and less correlation between the long procedure and the Ittrich. When the long and the short method are compared there is a high correlation with less variation in individual values.

Diurnal variation of estriol excretion in human pregnancy

Abstract Twenty-one normally pregnant patients had their urinary output fractionated into 4 hour aliquots for a total of 6 days. Each urinary specimen was analyzed for estriol. By using duplicate assays the statistical error was reduced to between 3 and 5 per cent. From these data it is not possible to predict the 24 hour urinary estriol excretion from a single 4 hour period specimen. Even the use of two 4 hour specimens in combination will not successfully predict the 24 hour excretion rate. While diurnal variation clearly exists, it is extremely complex and not simply predictable for a given patient. In these normally pregnant patients, bed rest had no significant influence on urinary estriol excretion rates.

RNAs as Candidate Diagnostic and Prognostic Markers of Prostate Cancer—From Cell Line Models to Liquid Biopsies

The treatment landscape of prostate cancer has evolved rapidly over the past five years. The explosion in treatment advances has been witnessed in parallel with significant progress in the field of molecular biomarkers. The advent of next-generation sequencing has enabled the molecular profiling of the genomic and transcriptomic architecture of prostate and other cancers. Coupled with this, is a renewed interest in the role of non-coding RNA (ncRNA) in prostate cancer biology. ncRNA consists of several different classes including small non-coding RNA (sncRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA). These families are under active investigation, given their essential roles in cancer initiation, development and progression. This review focuses on the evidence for the role of RNAs in prostate cancer, and their use as diagnostic and prognostic markers, and targets for treatment in this disease.

A Neonate and Her Diabetic Mother

At the University of Kentucky Albert B. Chandler Medical Center, Integrative Clinicopathological Conferences are held regularly to explore various dimensions—economic, psychosocial, ethical, preventive, and medical—of health care. They are organized by Dr. Joseph Engelberg. Professor of Physiology and Biophysics. This conference was moderated by Dr. Peter P. Bosomworth. Chancellor of the Medical Center.