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Dive into the research topics where John W. Holaday is active.

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Featured researches published by John W. Holaday.


Vaccine | 2000

Administration of a liposomal FGF-2 peptide vaccine leads to abrogation of FGF-2-mediated angiogenesis and tumor development

Stacy M. Plum; John W. Holaday; Antonio Ruiz; John W. Madsen; William E. Fogler; Anne H. Fortier

Basic fibroblast growth factor (FGF-2) is an important stimulator of angiogenesis that has been implicated in neoplastic progression. Attempts to neutralize or modulate FGF-2 have met with some success in controlling neovascularity and tumor growth. In the present study, two peptides: one corresponding to the heparin binding domain and the other to the receptor binding domain of FGF-2, exerted dose-dependent inhibition of FGF-2-stimulated human umbilical vein endothelial cell proliferation (IC(50)=70 and 20 microg/ml, respectively). The identification of these functional regions suggested that targeting these domains might be an approach for the modulation of FGF-2 function. To investigate this possibility, we vaccinated mice with either the heparin binding domain peptide or the receptor binding domain peptide of FGF-2 in a liposome/adjuvant format, and analyzed the effect of vaccination on FGF-2-driven angiogenesis, tumor development and immune status. Mice vaccinated with the heparin binding domain peptide generated a specific antibody response to FGF-2, blocked neovascularization in a gelfoam sponge model of angiogenesis, and inhibited experimental metastasis by >90% in two tumor models: the B16BL6 melanoma and the Lewis lung carcinoma. These effects were not observed in mice treated with the receptor binding domain peptide conjugated to liposomes or liposomes lacking conjugated peptide. These data suggest that a heparin binding domain peptide of FGF-2, when presented to a host in a liposomal adjuvant formulation, can ultimately lead to inhibition of angiogenesis and tumor growth.


Journal of the National Cancer Institute | 1999

Antiangiogenic activity of prostate-specific antigen.

Anne H. Fortier; Barbara J. Nelson; Davida K. Grella; John W. Holaday


Archive | 1996

Flow electroporation chamber and method

John W. Holaday; Peter H. Meserol; Doug Doerfler; Shawn J. Green; Vininder Singh


Archive | 2002

Apparatus and method for electroporation of biological samples

Sergey Dzekunov; Hyung J. Lee; Linhong Li; Vininder Singh; Linda Liu; John W. Holaday


Archive | 1994

Compositions and methods for treating cancer and hyperproliferative disorders

Carol A. Nacy; John W. Holaday


Archive | 2001

Compositions and methods for inhibiting endothelial cell proliferation and regulating angiogenesis using cancer markers

John W. Holaday; Anne H. Fortier


Shock | 1996

HANDBOOK OF MEDIATORS IN SEPTIC SHOCK

E. Neugebauer; John W. Holaday


Archive | 1999

Compositions and methods for inhibiting endothelial cell proliferation and regulating angiogenesis using serine proteases

John W. Holaday; Anne H. Fortier


Journal of the National Cancer Institute | 2000

Response: Re: Antiangiogenic Activity of Prostate-Specific Antigen

Anne H. Fortier; Davida K. Grella; Barbara J. Nelson; John W. Holaday


Archive | 2002

Appareil et procede d'electroporation d'echantillons biologiques

Sergey Dzekunov; Hyung J. Lee; Linhong Li; Vininder Singh; Linda Liu; John W. Holaday

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Carol A. Nacy

Walter Reed Army Institute of Research

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Linhong Li

Center for Cell and Gene Therapy

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Shawn J. Green

Georgetown University Medical Center

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John W. Madsen

National Institutes of Health

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William E. Fogler

Walter Reed Army Institute of Research

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