John Woolliams

ANIMAL AND DIETARY VARIATION IN THE ABSORPTION AND METABOLISM OF PHOSPHORUS BY SHEEP

In Expt 1 the efficiency of absorption of phosphorus in feedingstuffs was compared within four sets of 18-month-old, chimaera-derived triplets; 12 feedingstuffs were evaluated in four balance trials with 32 P. Seven diets consisted of a P-rich ingredient mixed with a low-P basic diet and five of hay or pelleted dried grass or lucerne. The P ingredients tested were the protein-rich meals, rape, soya-bean, maize gluten, fishmeal and rice bran, and the cereals, barley and wheat. Sets of triplets absorbed dietary P with different (P Endogenous faecal excretion was positively related to intake (P Significant urinary excretion of P generally occurred when the efficiency of absorption was greater than 0·70. Two members of a set which absorbed P with high efficiency and excreted low volumes of urine died from urethral obstruction caused by calculi. In Expt 2 an estimate of the variation in the concentration of P in plasma of 48 ewes on a barley-based diet was assessed and the values for three of the chimaera sets fell within the top quartile. Safety factors were calculated from the animal and plant variations in the efficiency of absorption of dietary P and used to determine dietary allowances for different classes of sheep.

The effect of breed of sire on the accumulation of copper in lambs, with particular reference to copper toxicity

Lambs, 9 weeks of age, the offspring of sires of the Scottish Blackface, East Friesland, Finnish Landrace, Suffolk and Texel breeds mated to Scottish Blackface females, as a common maternal breed, were given diets containing 12 or 20 mg copper per kg dry matter for 13 weeks. Concentrations of liver copper and of copper and aspartate amino-transferase in the plasma were measured regularly, and the concentrations of copper in the kidney, mandible and cheek muscle were also measured terminally. Livers were examined for histological symptoms of copper toxicity. Wide breed variation was apparent in liver copper concentration after 6 weeks and in plasma aspartate aminotransferase concentration after 9 weeks. At slaughter, total liver copper and kidney copper concentration showed significant breed variation. For all traits, the Texel-cross lambs had the highest concentrations, followed by the Suffolk crosses, and the Blackface had the lowest. The proportion of ingested copper retained in the liver of the Texel-cross lambs was twice that of the Blackface lambs (0·137 v . 0·056), with the other breed crosses intermediate. There was a significant difference between diets for liver, kidney and mandible copper concentrations, plasma aspartate amino-transferase concentration, and for total liver copper but not for cheek muscle. Both diets produced elevated plasma aspartate amino-transferase concentration and histological symptoms of copper toxicity in the livers of some lambs. The results show large breed differences in the susceptibility to copper poisoning, some breeds being at risk when given diets containing 12mg copper per kg dry matter for long periods. There was no suggestion that breeds differed in resistance to poisoning other than through different retentions of copper consumed

BREED DIFFERENCES IN COPPER-METABOLISM IN SHEEP

Evidence was sought for genetic differences in the absorption or systemic use of copper by comparing responses in plasma Cu concentration to oral and intravenous Cu repletion. Lambs of the North Ronaldsay (Orkney), Scottish Blackface and Welsh Mountain breeds and crosses of these with the North Ronaldsay were used.In two oral repletion phases average Cu intakes/kg live weight were 0·87 and 0·63 mg/week and similar for all breed groups. Plasma Cu concentrations of Scottish Blackface lambs showed little or no increase while those of North Ronaldsay lambs increased rapidly, and those of Welsh Mountain lambs increased moderately. The plasma Cu concentrations of the cross-breds increased more rapidly in the first than in the second phase; their rate of change lay between the pure breeds but was closer to that of the North Ronaldsay in the first phase.The rates of increase in plasma Cu concentration during intravenous repletion were relatively uniform for the different breeds and crosses except the Blackface which showed a lower rate. It was concluded that the large differences between groups in response to oral Cu repletion were largely attributable to differences in the efficiency of Cu absorption.

THE COPPER CONTENT OF WOOL IN RELATION TO BREED AND THE CONCENTRATIONS OF COPPER IN THE LIVER AND PLASMA

Little reliable information is available on the range and variability of the copper content of the wool of sheep (Underwood, 1977), although the effect of Cu deficiency on keratinization and pigmentation of wool is well known (Ryder & Stephenson, 1968) and the Cu content of hair has been suggested as a possible aid in diagnosis of hypocuprosis. Results from Kellaway, Sitorus & Leibholz (1978) showed that, in cattle, Cu in hair and plasma decreased in animals when the concentration of Cu in the liver fell below 20 mg/kg D.M. An experiment carried out jointly by the Animal Breeding Research Organisation and the Animal Diseases Research Association to investigate breed differences in the long-term accumulation of Cu in sheep gave an opportunity to investigate both the range of Cu concentrations in wool and the relationship between the Cu content of the liver and wool Cu.

The effects of breed, crossbreeding and other factors on variation in fleece and skin traits

Breed and crossbreeding significantly affected variation in 10 fleece, follicle density and fibre diameter traits in 12-week-old lambs. The breeds involved were the Scottish Blackface, South Country Cheviot, Welsh Mountain, Lincoln Longwool, Southdown and Tasmanian Merino, but the last only by rams. The Southdown came closest to the Merino in affecting density and diameter traits although the Merino crosses remained clearly distinguishable by their high follicle density. The Lincoln and Blackface crosses were notable for their long fibres and the Blackface crosses also had the least dense follicle and coarsest primary fibres. The coarsest secondary fibres were from Lincoln and Cheviot crosses. Heterosis estimates were obtained for crosses involving the Blackface, Cheviot, Welsh and Lincoln breeds. Significant heterosis was present in a wide range of traits but was not apparent in all crosses. Maternal differences were evident for many of the traits but with density these differences were apparently related to maternal effects on live weight of the lamb. The sex of the lamb, its birth type and rearing and its 12-week live weight significantly affected many of the traits. Among breeds and crosses a strong relationship was found between fibre density and average fibre diameter. The relationship suggested that breed variation in total fibre area per unit area of skin was small compared with the breed variation in its components. Fleece weight per unit area was most closely related to mean fibre length.