Jon M. Davenport

Estimating abundance in the presence of species uncertainty

Summary N-mixture models have become a popular method for estimating abundance of free-ranging animals that are not marked or identified individually. These models have been used on count data for single species that can be identified with certainty. However, co-occurring species often look similar during one or more life stages, making it difficult to assign species for all recorded captures. This uncertainty creates problems for estimating species-specific abundance, and it can often limit life stages to which we can make inference. We present a new extension of N-mixture models that accounts for species uncertainty. In addition to estimating site-specific abundances and detection probabilities, this model allows estimating probability of correct assignment of species identity. We implement this hierarchical model in a Bayesian framework and provide all code for running the model in BUGS language programs. We present an application of the model on count data from two sympatric freshwater fishes, the brook stickleback (Culaea inconstans) and the ninespine stickleback (Pungitius pungitius), and illustrate implementation of covariate effects (habitat characteristics). In addition, we used a simulation study to validate the model and illustrate potential sample size issues. We also compared, for both real and simulated data, estimates provided by our model to those obtained by a simple N-mixture model when captures of unknown species identification were discarded. In the latter case, abundance estimates appeared highly biased and very imprecise, while our new model provided unbiased estimates with higher precision. This extension of the N-mixture model should be useful for a wide variety of studies and taxa, as species uncertainty is a common issue. It should notably help improve investigation of abundance and vital rate characteristics of organisms’ early life stages, which are sometimes more difficult to identify than adults.

Evidence for asymmetric competition among headwater stream vertebrates

The importance of competition among stream salamanders and other stream vertebrates in headwater systems is understudied. We conducted a replicated artificial stream experiment to evaluate competitive interactions among three common vertebrates. In this experiment, we measured change in body condition of salamanders in the black-bellied dusky complex (Desmognathus quadramaculatus/folkertsi) in the presence of two different fish species, Common Creek Chub (Semotilus atromaculatus) and Coosa Darter (Etheostoma coosae). There was no statistically significant change in body condition between the control and darter present treatments. However, salamander body condition was significantly reduced in the presence of the creek chubs suggesting an asymmetric competitive interaction between those two species. While predation is often cited as a potential mechanism limiting the distribution of stream species, the role of interspecific competition may be just as vital. Overall, our results highlight that competition, and not solely predation, may explain why some stream salamanders are restricted to headwater reaches.

The Interactive Effects of Fish Predation and Conspecific Density on Survival and Growth of Tadpoles of Rana sylvatica in a Subarctic Wetland

In nature, prey are often simultaneously exposed to predators and competitors, leading to conflicting demands between survival and growth. Larval amphibians are model organisms to investigate the balancing of life history trade-offs because individuals must metamorphose before a wetland dries. Therefore, we deployed enclosures in a natural wetland to investigate the interactive effects of Wood Frog (Rana sylvatica) density (50 or 100 tadpoles) and Brook Stickleback (Culaea inconstans) presence on survival and growth of tadpoles. We hypothesized that the effects of conspecific density on tadpole survival and growth would be dependent on the presence of fish predators. Stickleback predation significantly decreased Wood Frog survival; however, a trend of higher tadpole survival with fish was detected at high conspecific densities. Wood Frog time to and mass at metamorphosis was not significantly affected by either conspecific density or stickleback presence. Our results demonstrate that Brook Sticklebacks can play a significant role in regulating Wood Frog survival in a Subarctic wetland, but the impact is likely dependent on tadpole density. Therefore, this research highlights the dynamic and complex effects of competition and predation in a Subarctic wetland. Our study also contributes to the limited understanding of factors affecting amphibian community structure in Subarctic wetlands.

Complex inter‐Kingdom interactions: carnivorous plants affect growth of an aquatic vertebrate

Coexistence of organisms in nature is more likely when phenotypic similarities of individuals are reduced. Despite the lack of similarity, distantly related taxa still compete intensely for shared resources. No larger difference between organisms that share a common prey could exist than between carnivorous plants and animals. However, few studies have considered inter-Kingdom competition among carnivorous plants and animals. In order to evaluate interactions between a carnivorous plant (greater bladderwort, Utricularia vulgaris) and a vertebrate (bluegill, Lepomis macrochirus) on a shared prey (zooplankton), we conducted a mesocosm experiment. We deployed two levels of bladderwort presence (functional and crushed) and measured bluegill responses (survival and growth). Zooplankton abundance was reduced the greatest in bluegill and functional bladderwort treatments. Bluegill survival did not differ among treatments, but growth was greatest with crushed bladderwort. Thus, bluegill growth was facilitated by reducing interference competition in the presence of crushed bladderwort. The facilitating effect was dampened, however, when functional bladderwort removed a shared prey. To our knowledge, this is one of the first studies to experimentally demonstrate interactions between a carnivorous plant and a fish. Our data suggest that carnivorous plants may actively promote or reduce animal co-occurrence from some ecosystems via facilitation or competition.

Post-breeding movement and habitat use by wood frogs along an Arctic–Subarctic ecotone

ABSTRACT By altering essential micro- and macrohabitat conditions for many organisms, climate change is already causing disproportionately greater impacts on Arctic and Subarctic ecosystems. Yet there is a lack of basic information about many species in northern latitudes, including amphibians. We used radio telemetry to study the post-breeding movements and habitat use of wood frogs (Rana [=Lithobates] sylvatica) in the Hudson Bay Lowlands near Churchill, Manitoba, Canada. We tracked fifty-seven frogs (thirty-five males, twenty-two females; mean duration = 16.8 d) from three wetlands during the summers of 2015 and 2016. The three wetlands were representative of the Arctic–Subarctic ecotone, with each wetland surrounded by different proportions of boreal forest and tundra. Our results indicate that at the landscape scale, movement distances increased with temperature, and all frogs spent more time in the tundra habitat than in boreal forests, relative to the availability of each habitat type. At the microhabitat scale (1 m2 plots), frogs selected areas with greater amounts of standing water, sedge, and shrubs. These results provide information on terrestrial movement patterns and critical habitat data for northern populations of wood frogs in a Subarctic environment, which will aid in understanding how climate change will affect amphibians in this rapidly changing ecosystem.

Amphibians and Reptiles

Abstract Amphibians and reptiles play important roles in their ecosystems because of their roles as prey and predators, their contributions to terrestrial-aquatic linkages, and their biomasses. Despite these important roles, their ecology in lotic systems can be challenging to study because the cryptic nature of their ecology makes it difficult to detect, capture, or recapture individuals. This chapter describes both standard and advanced techniques for quantifying occupancy, survival, and abundance of amphibians and reptiles. This chapter includes investigations into (1) factors that cause variation or biases in detection and (2) capture probabilities that can alter the inferences drawn from a study of these organisms. Quantifying low detection and capture probabilities to estimate high densities illustrates the overlooked significance of these organisms in the function and integrity of aquatic systems.

The effects of sunfish on spotted salamander oviposition, hatching time, and larval survival

Introduced species have negative effects on amphibian populations by reducing local recruitment and consuming larvae before metamorphosis. Fish can naturally colonize ponds periodically because of flooding and connectivity with river floodplains. However, many fish introductions have been intentional for commercial, recreational, and biocontrol purposes. In southeast Missouri (USA), Ambystoma maculatum will attempt to breed in ponds even if introduced fish ( Lepomis spp.) are present. We predicted that fish would have negative effects on salamander oviposition, hatching success and timing, and larval survival. In order to evaluate the effects of introduced fish on A. maculatum across life stages, we conducted pond surveys and a series of mesocosm experiments. Using field surveys, A . maculatum deposited significantly fewer eggs in ponds with fish. In short-term mesocosm experiments, we found that hatching time was not significantly affected by deposition site or fish cues, however, hatching success was lower for eggs deposited in fish ponds. No A. maculatum larvae survived when fish were present, regardless of egg deposition site. Our study is important because we found that, unless a female avoids depositing eggs with fish, one fish species can have profound effects on larval amphibian persistence. Therefore, small-scale fish introductions for recreation can act as a potential source for reduced recruitment and an increased risk of local extinction.