Jonathan Heddle
Yokohama City University
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Publication
Featured researches published by Jonathan Heddle.
Journal of Biological Chemistry | 2005
Ben R. Otto; Robert Sijbrandi; Joen Luirink; Bauke Oudega; Jonathan Heddle; Kenji Mizutani; Sam-Yong Park; Jeremy R. H. Tame
The acquisition of iron is essential for the survival of pathogenic bacteria, which have consequently evolved a wide variety of uptake systems to extract iron and heme from host proteins such as hemoglobin. Hemoglobin protease (Hbp) was discovered as a factor involved in the symbiosis of pathogenic Escherichia coli and Bacteroides fragilis, which cause intra-abdominal abscesses. Released from E. coli, this serine protease autotransporter degrades hemoglobin and delivers heme to both bacterial species. The crystal structure of the complete passenger domain of Hbp (110 kDa) is presented, which is the first structure from this class of serine proteases and the largest parallel β-helical structure yet solved.
Journal of Biological Chemistry | 2003
Jonathan Heddle; David J. Scott; Satoru Unzai; Sam-Yong Park; Jeremy R. H. Tame
Bacteria have evolved a number of tightly controlled import and export systems to maintain intracellular levels of the essential but potentially toxic metal nickel. Nickel homeostasis systems include the dedicated nickel uptake system nik found in Escherichia coli, a member of the ABC family of transporters, that involves a periplasmic nickel-binding protein, NikA. This is the initial nickel receptor and mediator of the chemotactic response away from nickel. We have solved the crystal structure of NikA protein in the presence and absence of nickel, showing that it behaves as a “classical” periplasmic binding protein. In contrast to other binding proteins, however, the ligand remains accessible to the solvent and is not completely enclosed. No direct bonds are formed between the metal cation and the protein. The nickel binding site is apolar, quite unlike any previously characterized protein nickel binding site. Despite relatively weak binding, NikA is specific for nickel. Using isothermal titration calorimetry, the dissociation constant for nickel was found to be ∼10 μm and that for cobalt was approximately 20 times higher.
Journal of Molecular Biology | 2007
Jonathan Heddle; Tomoyuki Okajima; David J. Scott; Satoko Akashi; Sam-Yong Park; Jeremy R. H. Tame
Journal of Biomolecular NMR | 2005
Sundaresan Rajesh; Jonathan Heddle; Kaori Kurashima-Ito; Daniel Nietlispach; Masahiro Shirakawa; Jeremy R. H. Tame; Yutaka Ito
Archive | 2006
Jonathan Heddle; Jeremy R. H. Tame
生物物理 | 2010
Satoru Unzai; Masahiro Watanabe; Satoko Akashi; Jonathan Heddle; S.-Y. Park; Jeremy R.H. Tame
生物物理 | 2008
Satoru Unzai; Masahiro Watanabe; Ken-ichi Kikuchi; Satoko Akashi; Jonathan Heddle; S.-Y. Park; Jeremy R.H. Tame
Acta Crystallographica Section A | 2008
Satoko Akashi; Masahiro Watanabe; Jonathan Heddle; Satoru Unzai; Sam-Yong Park; Jeremy R. H. Tame
Acta Crystallographica Section A | 2008
Masahiro Watanabe; Jonathan Heddle; Satoru Unzai; Satoko Akashi; Sam-Yong Park; Jeremy R. H. Tame
生物物理 | 2005
勇 藤原; 重雄 吉井; 和晃 西尾; Jonathan Heddle; 一郎 山下