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Featured researches published by Jonathan Heddle.


Journal of Biological Chemistry | 2005

Crystal structure of hemoglobin protease, a heme binding autotransporter protein from pathogenic Escherichia coli

Ben R. Otto; Robert Sijbrandi; Joen Luirink; Bauke Oudega; Jonathan Heddle; Kenji Mizutani; Sam-Yong Park; Jeremy R. H. Tame

The acquisition of iron is essential for the survival of pathogenic bacteria, which have consequently evolved a wide variety of uptake systems to extract iron and heme from host proteins such as hemoglobin. Hemoglobin protease (Hbp) was discovered as a factor involved in the symbiosis of pathogenic Escherichia coli and Bacteroides fragilis, which cause intra-abdominal abscesses. Released from E. coli, this serine protease autotransporter degrades hemoglobin and delivers heme to both bacterial species. The crystal structure of the complete passenger domain of Hbp (110 kDa) is presented, which is the first structure from this class of serine proteases and the largest parallel β-helical structure yet solved.


Journal of Biological Chemistry | 2003

Crystal structures of the liganded and unliganded nickel-binding protein NikA from Escherichia coli

Jonathan Heddle; David J. Scott; Satoru Unzai; Sam-Yong Park; Jeremy R. H. Tame

Bacteria have evolved a number of tightly controlled import and export systems to maintain intracellular levels of the essential but potentially toxic metal nickel. Nickel homeostasis systems include the dedicated nickel uptake system nik found in Escherichia coli, a member of the ABC family of transporters, that involves a periplasmic nickel-binding protein, NikA. This is the initial nickel receptor and mediator of the chemotactic response away from nickel. We have solved the crystal structure of NikA protein in the presence and absence of nickel, showing that it behaves as a “classical” periplasmic binding protein. In contrast to other binding proteins, however, the ligand remains accessible to the solvent and is not completely enclosed. No direct bonds are formed between the metal cation and the protein. The nickel binding site is apolar, quite unlike any previously characterized protein nickel binding site. Despite relatively weak binding, NikA is specific for nickel. Using isothermal titration calorimetry, the dissociation constant for nickel was found to be ∼10 μm and that for cobalt was approximately 20 times higher.


Journal of Molecular Biology | 2007

Dynamic allostery in the ring protein TRAP.

Jonathan Heddle; Tomoyuki Okajima; David J. Scott; Satoko Akashi; Sam-Yong Park; Jeremy R. H. Tame


Journal of Biomolecular NMR | 2005

Backbone 1H, 13C, and 15N assignments of a 56 kDa E. coli nickel binding protein NikA.

Sundaresan Rajesh; Jonathan Heddle; Kaori Kurashima-Ito; Daniel Nietlispach; Masahiro Shirakawa; Jeremy R. H. Tame; Yutaka Ito


Archive | 2006

Modified TRAP protein for producing nano-scale electrical devices, and a method for producing such a protein

Jonathan Heddle; Jeremy R. H. Tame


生物物理 | 2010

3P029 超遠心分析沈降速度法を用いたTRAP-antiTRAP相互作用解析(蛋白質-構造機能相関,第48回日本生物物理学会年会)

Satoru Unzai; Masahiro Watanabe; Satoko Akashi; Jonathan Heddle; S.-Y. Park; Jeremy R.H. Tame


生物物理 | 2008

1P-056 超遠心分析沈降速度法を用いたTRAP-antiTRAP相互作用解析(蛋白質・構造機能相関(1),第46回日本生物物理学会年会)

Satoru Unzai; Masahiro Watanabe; Ken-ichi Kikuchi; Satoko Akashi; Jonathan Heddle; S.-Y. Park; Jeremy R.H. Tame


Acta Crystallographica Section A | 2008

Studies of protein–protein and protein–RNA complexes by mass spectrometry

Satoko Akashi; Masahiro Watanabe; Jonathan Heddle; Satoru Unzai; Sam-Yong Park; Jeremy R. H. Tame


Acta Crystallographica Section A | 2008

Nature of the TRAP:Anti-TRAP complex revealed by symmetry remodeling

Masahiro Watanabe; Jonathan Heddle; Satoru Unzai; Satoko Akashi; Sam-Yong Park; Jeremy R. H. Tame


生物物理 | 2005

3P339 システインを導入したTRAPによる基板上Auナノ粒子の固定(バイオエンジニアリング))

勇 藤原; 重雄 吉井; 和晃 西尾; Jonathan Heddle; 一郎 山下

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Sam-Yong Park

Yokohama City University

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Satoko Akashi

Yokohama City University

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Satoru Unzai

Yokohama City University

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Ben R. Otto

VU University Amsterdam

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David J. Scott

University of Nottingham

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Satoru Unzai

Yokohama City University

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Jeremy R.H. Tame

University of Pennsylvania

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