Jonathan Rosa Moreira

Evaluation of the efficacy of systemic miltefosine associated with photodynamic therapy with liposomal chloroaluminium phthalocyanine in the treatment of cutaneous leishmaniasis caused by Leishmania (L.) amazonensis in C57BL/6 mice.

BACKGROUND The shortage of drugs is a concern and has become the object of studies to discover effective alternatives for cutaneous leishmaniasis (CL) treatment. A topical formulation has been sought due to its low toxicity. Development of alternative therapies, such as multimodal ones, is important in confronting drug resistance. This study aims to compare the in vivo efficacy of topical photodynamic therapy (PDT) using liposomal chloroaluminium phthalocyanine (AlClPC) in the treatment of CL, isolated and associated with systemic therapy with miltefosine. METHODS Five groups were adopted, each one with six isogenic adult female mice C57BL/6: (1) Negative Control-non-infected and non-treated; (2) Positive Control (PBS)-infected and non-treated; (3) Miltefosine-infected and treated with oral miltefosine 200 mg/kg/day; (4) Infected and treated with PDT with topical AlClPC (500 μL) on alternate days; (5) Oral Miltefosine 200 mg/kg/day and PDT with topical AlClPC (500 μL) on alternate days. Therapeutic schemes lasted 20 days. Infection was confirmed by culture in Nove-McNeal-Nicolle medium (NNN) of lymph collected from the animal paw, and animals were evaluated by paw measurement and parasitological criteria. RESULTS Miltefosine associated with PDT with AlClPC promoted a significant reduction in parasite number and viability when compared to the other infected groups, also returning the paw diameter to a size similar to the negative control group after 20 days of treatment. CONCLUSIONS Association of miltefosine with PDT mediated by topical AlClPC represents hopes for CL treatment, an increasing dermatological disease in some countries.

Avaliação dos Espaços de Construção de Conhecimento da Escola de Tecnologia da Faculdade Projeção de Brasília

O conhecimento tem assumido papel de destaque nas organizacoes e o seu valor reflete diretamente na competitividade e posicionamento no mercado. A falta de entendimento acerca dos processos e de como se estabelece uma comunicacao eficaz pode impactar no aprendizado e na cultura organizacionais das Instituicoes de Educacao Superior. Este artigo visa contribuir com um estudo que apresente como a relacao entre Comunicacao Organizacional, Gestao do Conhecimento, Aprendizagem Organizacional, e Cultura Organizacional pode corresponder um espaco de Construcao de Conhecimento para uma Instituicao de Educacao Superior privada. Trata-se de uma pesquisa com abordagem qualitativa, aplicada no âmbito da Faculdade Projecao, Distrito Federal, para os membros da comunidade academica envolvidos em sua Escola de Tecnologia. Em funcao da revisao de literatura e da analise documental, espera-se propor um modelo de construcao de conhecimento para a instituicao e avaliar como a Escola de Tecnologia se comporta nesse cenario.