Jong-Seok Kim

Functional analysis of expressed sequence tags from the liver and brain of Korean Jindo dogs

We generated 16,993 expressed sequence tags (ESTs) from two libraries containing full-length cDNAs from the brain and liver of the Korean Jindo dog. An additional 365,909 ESTs from other dog breeds were identified from the NCBI dbEST database, and all ESTs were clustered into 28,514 consensus sequences using StackPack. We selected the 7,305 consensus sequences that could be assembled from at least five ESTs and estimated that 12,533 high-quality single nucleotide polymorphisms (SNPs) were present in 97,835 putative SNPs from the 7,305 consensus sequences. We identified 58 Jindo dog-specific SNPs in comparison to other breeds and predicted seven synonymous SNPs and ten non-synonymous SNPs. Using PolyPhen, a program that predicts changes in protein structure and potential effects on protein function caused by amino acid substitutions, three of the non-synonymous SNPs were predicted to result in changes in protein function for proteins expressed by three different genes (TUSC3, ITIH2, and NAT2).

Genome-wide analysis of the diversity and ancestry of Korean dogs

There are various hypotheses on dog domestication based on archeological and genetic studies. Although many studies have been conducted on the origin of dogs, the existing literature about the ancestry, diversity, and population structure of Korean dogs is sparse. Therefore, this study is focused on the origin, diversity and population structure of Korean dogs. The study sample comprised four major categories, including non-dogs (coyotes and wolves), ancient, modern and Korean dogs. Selected samples were genotyped using an Illumina CanineHD array containing 173,662 single nucleotide polymorphisms. The genome-wide data were filtered using quality control parameters in PLINK 1.9. Only autosomal chromosomes were used for further analysis. The negative off-diagonal variance of the genetic relationship matrix analysis depicted, the variability of samples in each population. FIS (inbreeding rate within a population) values indicated, a low level of inbreeding within populations, and the patterns were in concordance with the results of Nei’s genetic distance analysis. The lowest FST (inbreeding rate between populations) values among Korean and Chinese breeds, using a phylogenetic tree, multi-dimensional scaling, and a TreeMix likelihood tree showed Korean breeds are highly related to Chinese breeds. The Korean breeds possessed a unique and large diversity of admixtures compared with other breeds. The highest and lowest effective population sizes were observed in Korean Jindo Black (485) and Korean Donggyeong White (109), respectively. The historical effective population size of all Korean dogs showed declining trend from the past to present. It is important to take immediate action to protect the Korean dog population while conserving their diversity. Furthermore, this study suggests that Korean dogs have unique diversity and are one of the basal lineages of East Asian dogs, originating from China.

Study of Jindo Dog Personality Traits:Questionnaire of The 16th Korean Jindo Dog Show

ABSTRACT There have been studies about dog’s personality and behavior, which is helpful to breed dogs as guide or companion. In this study, a questionnaire was developed using 54 Jindo dogs, which considered ten items about aggressiveness and sociability. The scores were analyzed by principle component analysis (PCA), after accounting for four variables: age, gender, growing place, and coat-colors. Our results from the PCA indicated three principle components, which classified ‘aggressiveness’, ‘sociability’ and unknown factor. The four variables did not significantly affect aggressiveness (P>0.05). However, there was a relationship between coat-color and sociability, i.e., the Jindo dogs with fawn color were more sociable than the white ones (P<0.1). ( Key words : Jindo dog, Personality, Coat-color, Aggressiveness, Sociability) . 서 론 개( Canis lufus familiaris )는 인간과 가장 가까이 지내는 동물이며, 인간에 의해서 다양한 외형적인 표현형을 가지는 종으로 개발되어 왔다 (Sutter와 Ostrander, 2004). 그러나 최근에는 개의 외형적인 표현형뿐만 아니라 그들이 가지는 행동이나 성격형질들이 과학자들의 관심을 받고 있다(Svartberg와 Forkman, 2002). 개의 성격에 대한 과학적인 연구를 위해 다양한 조사 방법들이 시도되고 있는데, 그 중에서 전문적인 사육사나 일반 애견가들에게 그들이 기르는 개

Effect of Cubed Roughage Supplementation and Red Clay Levels on Growth Performance, Meat Quality and Economic Benefits in Korean Native Cattle

This study was conducted to estimate the effects of high quality cubed roughage and red clay (0%, 1%, 2%) on growth performance, meat quality and economical benefits. Thirty-six 6 month-old Korean native cattle, averaging 156kg of BW, were assigned to six groups in a 2 (rice straw : RT, rice straw+cubed roughage : RCT) 3 (0, 1, 2% red clay). During experimental period, the high quality cubed roughage added into rice straw treatment (RCT) was superior to the only rice straw treatment (RT) on daily gain and feed efficiency and final weight 10%, 6.8%, 8.2% (P