Joo Youn Kim
Chungbuk National University
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Featured researches published by Joo Youn Kim.
Phytomedicine | 2012
Joo Youn Kim; Ha Yeon Jeong; Hong Kyu Lee; Seung-Hwan Kim; Bang Yeon Hwang; KiHwan Bae; Yeon Hee Seong
Vitis amurensis (Vitaceae) has been reported to have anti-oxidant and anti-inflammatory activities. The present study investigated a methanol extract from the leaf and stem of V. amurensis for neuroprotective effects on cerebral ischemic damage in rats and on excitotoxicity induced by glutamate in cultured rat cortical neurons. Transient focal cerebral ischemia was induced by 2h middle cerebral artery occlusion followed by 24h reperfusion (MCAO/reperfusion) in rats. Orally administered V. amurensis (25-100 mg/kg) reduced MCAO/reperfusion-induced infarct and edema formation, neurological deficits, and neuronal death. Depletion of glutathione (GSH) level and lipid peroxidation induced by MCAO/reperfusion was inhibited by administration of V. amurensis. The increase of phosphorylated mitogen-activated protein kinases (MAPKs), cyclooxygenase-2 (COX-2), and pro-apoptotic proteins and the decrease of anti-apoptotic protein in MCAO/reperfusion rats were significantly inhibited by treatment with V. amurensis. Exposure of cultured cortical neurons to 500 μM glutamate for 12h induced neuronal cell death. V. amurensis (1-50 μg/ml) and (+)-ampelopsin A, γ-2-viniferin, and trans-ε-viniferin isolated from the leaf and stem of V. amurensis inhibited glutamate-induced neuronal death, the elevation of intracellular calcium ([Ca(2+)](i)), the generation of reactive oxygen species (ROS), and changes of apoptosis-related proteins in cultured cortical neurons, suggesting that the neuroprotective effect of V. amurensis may be partially attributed to these compounds. These results suggest that the neuroprotective effect of V. amurensis against focal cerebral ischemic injury might be due to its anti-apoptotic effect, resulting from anti-excitotoxic, anti-oxidative, and anti-inflammatory effects and that the leaf and stem of V. amurensis have possible therapeutic roles for preventing neurodegeneration in stroke.
Archives of Pharmacal Research | 2010
Ha Yeon Jeong; Joo Youn Kim; Hong Kyu Lee; Do Thi Ha; Kyung-Sik Song; KiHwan Bae; Yeon Hee Seong
This study investigated a methanol extract from the leaf and stem of Vitis amurensis (Vitaceae) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25–35) in cultured rat cortical neurons and also for antidementia activity in mice. Exposure of cultured cortical neurons to 10 μM Aβ (25–35) for 36 h induced neuronal apoptotic death. At concentrations of 1–10 μg/mL, V. amurensis inhibited neuronal death, the elevation of intracellular calcium ([Ca2+]i) and the generation of reactive oxygen species (ROS), all of which were induced by Aβ (25–35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 16 nmol Aβ (25–35) was inhibited by chronic treatment with V. amurensis extract (50 and 100 mg/kg, p.o. for 7 days), as measured by a passive avoidance test. Amurensin G, r-2-viniferin and trans-ɛ-viniferin isolated from V. amurensis also inhibited neuronal death, the elevation of [Ca2+]i and the generation of ROS induced by Aβ (25–35) in cultured rat cortical neurons. These results suggest that the neuroprotective effect of V. amurensis may be partially attributable to these compounds. These results suggest that the antidementia effect of V. amurensis is due to its neuroprotective effect against Aβ (25–35)-induced neurotoxicity and that the leaf and stem of V. amurensis have possible therapeutic roles for preventing the progression of Alzheimer’s disease.
Archives of Pharmacal Research | 2012
Hong Kyu Lee; Eun-Ju Yang; Joo Youn Kim; Kyung-Sik Song; Yeon Hee Seong
This study investigated an ethanol extract from Glycyrrhizae radix (GR), the root of Glycyrrhiza uralensis (Leguminosae), for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25–35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to 10 μM Aβ (25–35) for 36 h induced neuronal apoptotic death. GR (10–50 μg/mL) prevented the Aβ (25–35)-induced neuronal apoptotic death, as assessed by a MTT assay and Hoechst 33342 staining. Furthermore, GR decreased the expression of Bax and active caspase-3, proapoptotic proteins, and increased Bcl-2, an antiapoptotic protein. GR also significantly inhibited Aβ (25–35)-induced elevation of the intracellular Ca2+ concentration ([Ca2+]i) and generation of reactive oxygen species (ROS) measured by fluorescent dyes. Isoliquiritigenin (1–20 μM), isolated from GR as an active component, inhibited Aβ (25–35)-induced neuronal apoptotic death, elevation of [Ca2+]i, ROS generation, and the change of apoptosis-associated proteins in cultured cortical neurons, suggesting that the neuroprotective effect of GR may be, at least partly, attributable to this compound. These results suggest that GR and isoliquiritigenin prevent Aβ (25–35)-induced neuronal apoptotic death by interfering with the increases of [Ca2+]i and ROS, and GR may have a possible therapeutic role for preventing the progression of neurodegenerative disease such as Alzheimer’s disease.
Archives of Pharmacal Research | 2009
Soon Ock Cho; Ju Yeon Ban; Joo Youn Kim; Hyun Soo Ju; IkSoo Lee; Kyung-Sik Song; KiHwan Bae; Yeon Hee Seong
Aralia has been reported to exhibit various pharmacological properties, including anti-inflammatory, antidiabetic and antioxidant activities. We performed in vitro and in vivo analyses on the neuroprotective effects of an ethanolic extract of the aerial parts of Aralia cordata Thunb. (Araliaceae). In cultured cortical neurons from rats, A. cordata (5–20 μg/mL) inhibited 100 μM hydrogen peroxide (H2O2)-induced apoptotic neuronal death, elevation of intracellular calcium concentration ([Ca2+]i) and generation of reactive oxygen species (ROS). Since oleanolic acid isolated from A. cordata also inhibited H2O2-induced neuronal death, increase in [Ca2+]i and ROS generation in cultured cortical neurons, some of the neuroprotective effects of A. cordata might be attributable to this compound. In rats, A. cordata prevented cerebral ischemic injury induced by 3 h of middle cerebral artery occlusion, followed by 24 h of reperfusion. Ischemic infarct and edema volumes were significantly reduced in rats that received A. cordata (50 mg/kg, orally). These animals exhibited a corresponding improvement in neurological function and a reduction of neuronal death, as determined histologically from the cortex and hippocampal regions. It is possible that the anti-oxidative properties of A. cordata may be responsible for its neuroprotective effects against focal cerebral ischemic injury. In future, A. cordata might play a therapeutic role in the prevention and treatment of neurodegeneration in stroke.
Archives of Pharmacal Research | 2012
Joo Youn Kim; Hong Kyu Lee; Bang Yeon Hwang; Seung-Hwan Kim; Jae Kuk Yoo; Yeon Hee Seong
Ilex latifolia (Aquifoliaceae), one of the primary components of “Ku-ding-cha”, has been used in Chinese folk medicine to treat headaches and various inflammatory diseases. A previous study demonstrated that the ethanol extract of I. latifolia could protect against ischemic apoptotic brain damage in rats. The present study investigated the protective activity of I. latifolia against glutamate-induced neurotoxicity using cultured rat cortical neurons in order to explain a possible mechanism related to its inhibitory effect on ischemic brain damage and identified potentially active compounds from it. Exposure of cultured cortical neurons to 500 μM glutamate for 12 h triggered neuronal cell death. I. latifolia (10–100 μg/mL) inhibited glutamate-induced neuronal death, elevation of intracellular calcium ([Ca2+]i), generation of reactive oxygen species (ROS), the increase of a pro-apoptotic protein, BAX, and the decrease of an anti-apoptotic protein, BcL-2. Hypoxia-induced neuronal cell death was also inhibited by I. latifolia. 3,4-Dicaffeoylquinic acid (diCQA), 3,5-diCQA, and 3,5-diCQA methyl ester isolated from I. latifolia also inhibited the glutamate-induced increase in [Ca2+]i, generation of ROS, the change of apoptosis-related proteins, and neuronal cell death; and hypoxia-induced neuronal cell death. These results suggest that I. latifolia and its active compounds prevented glutamate-induced neuronal cell damage by inhibiting increase of [Ca2+]i, generation of ROS, and resultantly apoptotic pathway. In addition, the neuroprotective effects of I. latifolia on ischemia-induced brain damage might be associated with the anti-excitatory and anti-oxidative actions and could be attributable to these active compounds, CQAs.
Journal of Ethnopharmacology | 2010
Eui-Ju Hong; Eui-Man Jung; Geun-Shik Lee; Joo Youn Kim; Ki-Jeong Na; Mi-Jin Park; Ha-Young Kang; Kyung-Chul Choi; Yeon Hee Seong; In-Gyu Choi; Eui-Bae Jeung
ETHNOPHARMACOLOGICAL RELEVANCE Bamboo species are thought to be originally from Central China, but are now found in many temperate and semi-tropical regions around the world. Although the extracts from bamboo may have antioxidant activities and anti-inflammatory effects, their exact biological activities have not been elucidated. AIM OF THE STUDY Two biological activities of bamboo-derived pyrolyzates were investigated; the protective effects against N-methyl-d-aspartate (NMDA)-induced cell death in primary cultured cortical neuron and the anti-plasmin effects determined by using fibrin and fibrinogen degradation products (FDPs) assay. RESULTS Treatment of neuronal cells with pyrolyzates of Phyllostachys pubescens, Phyllostachys nigra and Phyllostachys bambusoides resulted in restored cell viability when compared to untreated cells in an NMDA-induced neuronal cell death assay. In addition, cortical neurons treated with Phyllostachys pubescens and Phyllostachys nigra showed a reduction of apoptosis following exposure to NMDA, as determined by Hoechst 33342 staining. In addition, Phyllostachys nigra pyrolyzates also exhibited anti-plasmin action in a FDP assay. It is of interest to note that pyrolyzates exhibited activities of NMDA-receptor antagonist and antifebrin (ogen), since a combination of NMDA receptor antagonists, glucocorticosteroids, GABAergic drugs and heparin are useful for treatment in delayed postischemic injury. CONCLUSION Our results indicate that the pyrolyzates derived from bamboo may have anti-apoptotic effects, and can be useful as a supplement for ischemic injury treatment.
Korean Journal of Medicinal Crop Science | 2011
Joo Youn Kim; Kiyeon Kweon; Hong Kyu Lee; Seung Hwan Kim; Jae Kuk Yoo; Ki Hwan Bae; Yeon Hee Seong
Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to hydrogen peroxide () induced neuronal apoptotic death. At , HS0608 inhibited neuronal death, elevation of intracellular calcium concentration (), and generation of reactive oxygen species (ROS) induced by in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 mg/kg). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.
Journal of Pharmacological Sciences | 2009
Soon Ock Cho; Ju Yeon Ban; Joo Youn Kim; Ha Yeon Jeong; IkSoo Lee; Kyung-Sik Song; KiHwan Bae; Yeon Hee Seong
Journal of Ethnopharmacology | 2011
Joo Youn Kim; Ha Yeon Jeong; Hong Kyu Lee; Jae Kuk Yoo; KiHwan Bae; Yeon Hee Seong
Korean Journal of Medicinal Crop Science | 2009
Joo Youn Kim; Hyun Soo Ju; Ju Yeon Ban; Kyung-Sik Song; KiHwan Bae; Yeon Hee Seong