Jörg Kleiber

Mismatch Analysis by PNA Arrays

Array of probes immobilised on a solid support has attracted considerable interest as a convenient format for the rapid analysis of nucleic acids carrying multiple mutations (Saiki et al., 1989). Currently, microfabricated devices containing arrays of DNA probes are being developed, e.g. for the analysis of mutations in the HIV-1 drug-resistance gene (Lipshutz et al., 1995) cystic fibrosis (Cronin et al., 1996), Duchenne muscular dystrophy gene (Beattie et al., 1995), BRCA1 (Hacia et al., 1996) and analysis of gene expression (Schena et al., 1995; Ferguson et al., 1996).