Jorge Bohorquez

Generation of the 40-Hz auditory steady-state response (ASSR) explained using convolution.

OBJECTIVE In this study, the superposition theory of the 40-Hz auditory steady-state response (ASSR) generation is investigated using auditory brainstem response (ABR) and middle latency responses (MLRs) obtained with 40 Hz jittered sequences with the continuous loop averaging deconvolution (CLAD) algorithm. METHODS Click sequences at around 40 Hz with high (maximum length sequence), medium and low jitters were presented to normal hearing awake adult subjects monaurally. Overlapping MLR responses were deconvolved using the frequency domain CLAD method. In addition, conventional auditory MLRs at 4.88 Hz and ASSRs at 39.1 Hz were obtained in all subjects. Synthetic ASSRs were constructed using different rate and jitter MLRs as base recordings. Contributions of the primary components were investigated by wave elimination using phasors. RESULTS Findings indicate that the generation of the 40-Hz ASSRs can be explained successfully by the superposition of the ABR and MLR waves generated at that stimulation rate. N(a)-P(a) and N(b)-P(b) components of the MLR contribute about equally (45% each), while the wave V of the ABR contributes a lesser amount (10%). CONCLUSIONS Forty-Hertz ASSRs are composite responses generated by the superposition of the major waves of the ABR and the MLR. Dramatic amplitude increase of the ASSR at 40Hz is primarily due to the superposition of the resonating P(b) component to the P(a) wave. SIGNIFICANCE Several unexplained properties of the 40-Hz ASSR can be explained by the stimulus and brain state dependent characteristics of the slow ABR, the P(a) and the P(b) components of the MLR.

Signal-to-noise ratio and frequency analysis of continuous loop averaging deconvolution (CLAD) of overlapping evoked potentials

In this study, a frequency domain formulation of continuous loop averaging deconvolution (CLAD) of overlapping evoked potentials is developed and applied for the extraction of transient responses from recordings obtained at high stimulation rates. This formulation allows for a faster execution of CLAD by using fast Fourier transform algorithms. The frequency characteristics of the deconvolution filter depends exclusively on the stimulus sequence and determines whether the noncoherent noise is amplified or attenuated in different frequencies. A formula for calculating the signal-to-noise ratio (SNR) achieved by the deconvolution process is developed. The newly developed theory and the methodology is applied to the extraction of the auditory brainstem and middle latency responses using various sequences. The effects of the sequence used and the number of sweeps averaged in ongoing acquisition on SNR are examined by using single sweep recordings. The results verify the deconvolution theory and the methodology and show its limitations. Depending on the frequency characteristics of the sequence, the deconvolution process can amplify or attenuate the EEG noise. Proper selection of the stimulus sequence can increase the SNR enhancement obtained with conventional averaging.

Molecular mechanisms involved in cochlear implantation trauma and the protection of hearing and auditory sensory cells by inhibition of c-jun-N-terminal kinase signaling

To investigate the molecular mechanisms involved in electrode insertion trauma (EIT) and to test the otoprotective effect of locally delivered AM‐111.

Pb(P1) resonance at 40 Hz: Effects of high stimulus rate on auditory middle latency responses (MLRs) explored using deconvolution

OBJECTIVE In this study, the effects of high stimulus rate on middle latency response (MLR) components P(a) and P(b) (P(1) or P50) were studied using high rate clicks in normal hearing awake subjects were investigated. METHODS Five jittered click sequences at different mean rates (24.4, 39.1, 58.6, 78.1, 97.7Hz) were presented to 10 subjects. Overlapping MLRs were deconvolved using the frequency domain continuous loop averaging deconvolution (CLAD) [Ozdamar O., Bohórquez, J., Signal to noise ratio and frequency analysis of continuous loop averaging deconvolution (CLAD) of overlapping evoked potentials. J. Acoust. Soc. Am., 119:429-438, 2006]. In addition conventional auditory transient MLRs at 4.88Hz were obtained using conventional averaging. RESULTS P(a) amplitude, latency and waveshape remained fairly constant up to 78.1Hz. P(b) component, however, showed a variable waveshape with latencies covering a wide range (50-70ms) and N(b)-P(b) amplitudes increasing at 39.1 and 58.6Hz and decreasing at other rates. CONCLUSIONS Recordings show that both P(a) and P(b) MLR components can be consistently recorded at all rates up to 100Hz. P(b) amplitude shows an increase at around 40Hz showing a resonance at that frequency. SIGNIFICANCE The dramatic increase of the P(b) component at around 40Hz may account for the high amplitude of the 40Hz ASSR.

Wiener filter deconvolution of overlapping evoked potentials

Evoked potentials (EPs) typically contain components that last up to several seconds. In order to save time and study adaptation effects, stimuli are often presented at high stimulation rates. Unfortunately, such protocols often suffer from a response overlap problem. Techniques based on Continuous Loop Averaging Deconvolution (CLAD) have been developed to formulate circular convolution to restore the source EP. These techniques, however, are sensitive to the additive noise and do not perform well when stimulus sequences with poor noise attenuation properties are chosen. This study offers a solution to this problem by applying Wiener theory to obtain an optimal filter that adapts the ratio of noise-to-signal by estimating the power spectra of both noise and signal using non-parametric or parametric methods. Experiments are conducted on simulation data and Auditory Evoked potentials (AEPs) acquired from human subjects to demonstrate the validity of the proposed theory. The results show that the Wiener deconvolution method improves the quality of the responses acquired with sequences with poor noise attenuation in the presence of high noise levels compared to the straight CLAD method. The results are comparable to responses obtained by conventional and Maximum Length Sequence (MLS) methods.

Signal to noise ratio analysis of maximum length sequence deconvolution of overlapping evoked potentials.

In this study a general formula for the signal to noise ratio (SNR) of the maximum length sequence (MLS) deconvolution averaging is developed using the frequency domain framework of the generalized continuous loop averaging deconvolution procedure [Ozdamar and Bohórquez, J. Acoust. Soc. Am. 119, 429-438 (2006)]. This formulation takes advantage of the well known equivalency of energies in the time and frequency domains (Parsevals theorem) to show that in MLS deconvolution, SNR increases with the square root of half of the number of stimuli in the sweep. This increase is less than that of conventional averaging which is the square root of the number of sweeps averaged. Unlike arbitrary stimulus sequences that can attenuate or amplify phase unlocked noise depending on the frequency characteristics, the MLS deconvolution attenuates noise in all frequencies consistently. Furthermore, MLS and its zero-padded variations present optimal attenuation of noise at all frequencies yet they present a highly jittered stimulus sequence. In real recordings of evoked potentials, the time advantage gained by noise attenuation could be lost by the signal amplitude attenuation due to neural adaptation at high stimulus rates.

Auditory steady-state responses to 40-Hz click trains: Relationship to middle latency, gamma band and beta band responses studied with deconvolution

OBJECTIVE The nature of the auditory steady-state responses (ASSR) evoked with 40-Hz click trains and their relationship to auditory brainstem and middle latency responses (ABR/MLR), gamma band responses (GBR) and beta band responses (BBR) were investigated using superposition theory. Transient responses obtained by continuous loop averaging deconvolution (CLAD) and last click responses (LCR) were used to synthesize ASSRs and GBRs. METHODS ASSRs were obtained with trains of low jittered 40 Hz clicks presented monaurally and deconvolved using a modified CLAD. Resulting transient responses and modified LCRs were used to predict the ASSRs and the GBR. RESULTS The ABR/MLR obtained with deconvolution predicted accurately the steady portion of the ASSR but failed to predict its onset portion. The modified LCR failed to fully predict both portions. The GBRs were predicted by narrow band filtering of the ASSRs. Significant BBR activity was found both in the ASSRs and deconvolved ABR/MLRs. CONCLUSIONS Simulations using deconvolved ABR/MLRs obtained at 40 Hz predict fully the steady state but not the onset portion of the ASSRs, thus confirming the superposition theory. SIGNIFICANCE Click rate adaptation plays a significant role in ASSR generation with click trains and should be considered in evaluating convolved response generation theories.

A New Paradigm for the Design of Audible Alarms that Convey Urgency Information

ObjectiveThe current international standard (IEC 60601-1-8) stipulates that medical device audible alarms should be priority-encoded and validated for efficacy. Evidence suggests that the melodic alarms described in the standard are not functioning as originally intended. We present a multi-disciplinary, human factors paradigm for audible alarm development whereby urgency information is encoded via modulation of the physical characteristics of sounds. We also test the feasibility of this approach using information measures.MethodsWe designed series of experimental sounds that varied along controlled physical and acoustical dimensions. Subjects rated these sound series for perceived urgency. Based on these ratings, selected sounds from each series were assigned a␣priority category from ‹low’ to ‹high’ – we call these resulting sets of sounds ‹urgency-codecs’. The method of categorical judgments (based on information theory) was used to compare each urgency-codec for ability to convey urgency information.ResultsSubjects were consistent in their ratings of the three series of experimental sounds for perceived urgency. The urgency data pertaining to one of the series (harmonic interval) was successfully fit to a psychophysical empirical law. The urgency-codec derived from another sound series (melodic interval) was found to have the highest signal (correct interpretation of urgency level by subjects) transmission rate.ConclusionsThe proposed paradigm is feasible, and it offers an evidence-based strategy for alarm sound design and testing. This approach would be performed before implementation of new alarm sounds in clinical settings, and should result in development of alarm sounds that satisfy the requirements of priority-encoding and validation.

Neuromonitoring of cochlea and auditory nerve with multiple extracted parameters during induced hypoxia and nerve manipulation

A system capable of comprehensive and detailed monitoring of the cochlea and the auditory nerve during intraoperative surgery was developed. The cochlear blood flow (CBF) and the electrocochleogram (ECochGm) were recorded at the round window (RW) niche using a specially designed otic probe. The ECochGm was further processed to obtain cochlear microphonics (CM) and compound action potentials (CAP). The amplitude and phase of the CM were used to quantify the activity of outer hair cells (OHC); CAP amplitude and latency were used to describe the auditory nerve and the synaptic activity of the inner hair cells (IHC). In addition, concurrent monitoring with a second electrophysiological channel was achieved by recording compound nerve action potential (CNAP) obtained directly from the auditory nerve. Stimulation paradigms, instrumentation and signal processing methods were developed to extract and differentiate the activity of the OHC and the IHC in response to three different frequencies. Narrow band acoustical stimuli elicited CM signals indicating mainly nonlinear operation of the mechano-electrical transduction of the OHCs. Special envelope detectors were developed and applied to the ECochGm to extract the CM fundamental component and its harmonics in real time. The system was extensively validated in experimental animal surgeries by performing nerve compressions and manipulations.

Local drug delivery to conserve hearing: mechanisms of action of eluted dexamethasone within the cochlea

Introduction Dexamethasone is a synthetic corticosteroid that is routinely administered via intratympanic injections for the treatment of sudden idiopathic sensorineural hearing loss (Plontke et al., 2009) with or without accompanying oral steroid therapy (Seggas et al., 2010). This synthetic corticosteroid has also been shown to be highly effective in local therapy for the conservation of hearing in an animal model of electrode insertion trauma (EIT)-induced hearing loss (Vivero et al., 2008). Delivery of a corticosteroid directly to the cochlea during a soft surgery procedure for cochlear implantation for patients with residual hearing has been suggested to decrease intracochlear inflammation, secondary fibrosis, and to conserve hearing (Kiefer et al., 2004). The objective of the present study was to test a new approach for the direct delivery of a corticosteroid therapy to the perilymph of the scala tympani via a cochlear implant electrode (Staecker et al., 2010) coated with a polymer-containing dexamethasone base (DXMb) in an animal model of EIT-induced hearing loss. In addition, the efficacy of polymer-eluded DXMb to prevent hair cell (HC) death and the molecular mechanisms involved in this otoprotection that has been previously reported (Dinh et al., 2008) is summarized.