Jorge L. Chávez-Servín

Elevated Circulating LDL Phenol Levels in Men Who Consumed Virgin Rather Than Refined Olive Oil Are Associated with Less Oxidation of Plasma LDL

In human LDL, the bioactivity of olive oil phenols is determined by the in vivo disposition of the biological metabolites of these compounds. Here, we examined how the ingestion of 2 similar olive oils affected the content of the metabolic forms of olive oil phenols in LDL in men. The oils differed in phenol concentrations as follows: high (629 mg/L) for virgin olive oil (VOO) and null (0 mg/L) for refined olive oil (ROO). The study population consisted of a subsample from the EUROLIVE study and a randomized controlled, crossover design was used. Intervention periods lasted 3 wk and were preceded by a 2-wk washout period. The levels of LDL hydroxytyrosol monosulfate and homovanillic acid sulfate, but not of tyrosol sulfate, increased after VOO ingestion (P < 0.05), whereas the concentrations of circulating oxidation markers, including oxidized LDL (oxLDL), conjugated dienes, and hydroxy fatty acids, decreased (P < 0.05). The levels of LDL phenols and oxidation markers were not affected by ROO consumption. The relative increase in the 3 LDL phenols was greater when men consumed VOO than when they consumed ROO (P < 0.05), as was the relative decrease in plasma oxLDL (P = 0.001) and hydroxy fatty acids (P < 0.001). Plasma oxLDL concentrations were negatively correlated with the LDL phenol levels (r = -0.296; P = 0.013). Phenols in LDL were not associated with other oxidation markers. In summary, the phenol concentration of olive oil modulates the phenolic metabolite content in LDL after sustained, daily consumption. The inverse relationship of these metabolites with the degree of LDL oxidation supports the in vivo antioxidant role of olive oil phenolics compounds.

Comparison of Chemical Composition and Growth of Amaranth (Amaranthus hypochondriacus) between Greenhouse and Open Field Systems

Amaranth can be cultivated in greenhouses or open fields. The aim of this study was to compare the basic chemical composition and growth parameters of Amaranthus hypochondriacus grown in a greenhouse in comparison to the open field. Each cropping system was assigned an experimental area of 100 m. Cultivation was performed directly in irrigated furrows without fertilizers. The same soil type was used for both cropping systems. Number of leaves, plant height, stem diameter, length of seeds, panicle length, biomass and grain yield, grain area, percentage of popped and sprouted grain, and chemical composition were compared. The variables under greenhouse cultivation yielded superior results were grain yield (26.5%), biomass (61.2%), plant height (87.4%), stem diameter (24.3%), number of leaves (2.4%), panicle length (18.4%), percentage of popped grain (2%) and sprouted grain (7%) than field cultivation. Open field cultivation improved protein, fat and ash (9.0%, 17.6% and 43.1%, respectively), and grain size (22.5%) than greenhouse production. Thus, it may be concluded that greenhouse cultivation of A. hypochondriacus crop provides for higher yields, but inferior chemical composition values compared to the open field cultivation system.