José Antonio Fernández Prieto

Phylogeny and systematics of Micranthes (Saxifragaceae): an appraisal in European territories

Recent phylogenetic studies have shown that Saxifraga, as currently understood, must be divided into two genera: Saxifraga L. sensu stricto and Micranthes Haw. To better understand the evolutionary history of these two genera, we performed phylogenetic analyses inferred from the nuclear ribosomal sequences from the internal transcribed spacer and the sequences of the plastid DNA (rbcL). Our molecular data confirmed the monophyly of the genus Micranthes and the consistency of the existing systematic treatments based on morphological criteria. Moreover, Micranthes species native from the Iberian Peninsula (i.e. M. clusii, M. lepismigena and M. stellaris) should be included into Micranthes sect. Arabisa.

Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences

The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship with the widespread R. parnassiifolius, based on nuclear DNA (internal transcribed spacer, ITS) and plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK and ycf6-psbM). Maximum parsimony and Bayesian inference analyses revealed a well-supported clade formed by accessions of R. wettsteinii. Furthermore, our phylogenetic and network analyses supported previous hypotheses of a likely allopolyploid origin for R. wettsteinii between R. montenegrinus and R. parnassiifolius, with the latter as the maternal parent.

The Iberian endemic species Ranunculus cabrerensis Rothm.: an intricate history in the Ranunculus parnassiifolius L. polyploid complex

The orophilous plant, Ranunculus parnassiifolius L., is a polyploid complex that is widespread throughout the southern European mountains, where at least five taxa have traditionally been recognized. The aim of this study was to test whether R. parnassiifolius subsp. cabrerensis should be treated as an independent species of the R. parnassiifolius polyploid complex and constitutes, therefore, an evolutionary line in itself. To disentangle its evolutionary history and taxonomy, we used genome size estimation based on flow cytometric measurements (FCM), multivariate morphometric analyses, polymerase chain reaction-restriction fragment length polymorphisms (PCR–RFLPs) and subsequent sequencing of cpDNA regions (trnS, trnR-atpA, atpH-atpI). This study provides molecular and morphological evidence for the recognition of R. cabrerensis at the species level, rather than as an intraspecific taxon of R. parnassiifolius. Furthermore, it is concluded that those plants previously known as R. parnassiifolius subsp. muniellensis should be systematized at the subspecies level as R. cabrerensis subsp. muniellensis. This contribution highlights the benefit of combining diverse approaches to obtain knowledge about relict populations and for the implementation of suitable conservation measures.

The influence of European and American wild germplasm in hop (Humulus lupulus L.) cultivars

Microsatellite variation at the nuclear and chloroplast genomes was evaluated for wild European and wild American hops, in order to assess the genetic diversity and origin of cultivated hops. Seven nuclear loci and 32 chloroplast loci were used in the analysis of 182 hop accessions including wild European (68), wild American (48), and cultivars (66). A total of 116 alleles were identified using 7 nuclear microsatellites showing different averages of polymorphism and distribution in the wild American and European accessions and cultivars. Two main groups were established as revealed by several statistical analyses; one including European wild accessions and cultivars and a second group consisting of American wild accessions. Three polymorphic chloroplast microsatellite loci were detected, six alleles were scored which defined a total of five haplotypes that were exclusive or presented different distribution between American and European wild accessions. A major influence of the wild European haplotypes was detected among hop cultivars. To the best of our knowledge, this is the first work reporting the use of chloroplast microsatellites in hops.

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

Ranunculus cabrerensis is an endemic and endangered species of the Northwestern Iberian Peninsula. The molecular markers AFLP and ISSR were used to investigate the genetic diversity and population structure of four populations across its known distribution. Fifteen selective primer combinations of AFLP and seventeen ISSR primer combinations produced a total of 2830 and 103 unambiguously repeatable fragments respectively, of which 97.57 and 81.38% were polymorphic for both markers. The genetic diversity of R. cabrerensis at species level was high (HE = 0.294 by ISSR and HE = 0.191 by AFLP) and differentiation between sampled locations was also relatively high (GST = 0.316 and 0.158 by ISSR and AFLP analysis respectively) compared to other studies of endangered and rare species using the same techniques. The analysis of molecular variance (AMOVA) indicated that the main genetic variation was within sampled locations (73% by AFLP; 52% by ISSR), even though the variation among locations was also significant. Principal Coordinates, NeighborNet and Bayesian analyses revealed a weak but significant relationship between the genetic structures of different populations in R. cabrerensis, with gene flow acting as a homogenizing force that prevents stronger differentiation of populations. Finally, suggestions for conservation strategies to preserve the genetic resources of this species are outlined.

Self-Regulation, Cooperative Learning, and Academic Self-Efficacy: Interactions to Prevent School Failure

Learning to learn and learning to cooperate are two important goals for individuals. Moreover, self regulation has been identified as fundamental to prevent school failure. The goal of the present study was to assess the interactions between self-regulated learning, cooperative learning and academic self-efficacy in secondary education students experiencing cooperative learning as the main pedagogical approach for at least one school year. 2.513 secondary education students (1.308 males, 1.205 females), 12–17 years old (M = 13.85, SD = 1.29), enrolled in 17 different schools belonging to the National Network of Schools on Cooperative Learning in Spain agreed to participate. They all had experienced this pedagogical approach a minimum of one school year. Participants were asked to complete the cooperative learning questionnaire, the strategies to control the study questionnaire and the global academic self-efficacy questionnaire. Participants were grouped based on their perceptions on cooperative learning and self-regulated learning in their classes. A combination of hierarchical and κ-means cluster analyses was used. Results revealed a four-cluster solution: cluster one included students with low levels of cooperative learning, self-regulated learning and academic self-efficacy, cluster two included students with high levels of cooperative learning, self-regulated learning and academic self-efficacy, cluster three included students with high levels of cooperative learning, low levels of self-regulated learning and intermediate-low levels of academic self-efficacy, and, finally, cluster four included students with high levels of self-regulated learning, low levels of cooperative learning, and intermediate-high levels of academic self-efficacy. Self-regulated learning was found more influential than cooperative learning on students’ academic self-efficacy. In cooperative learning contexts students interact through different types of regulations: self, co, and shared. Educators should be aware of these interactions, symmetrical or asymmetrical, because they determine the quality and quantity of the students’ participation and achievements, and they are key elements to prevent school failure.

The Influence of Age on Aerobic Capacity and Health Indicators of Three Rescue Groups

The aim of this study was to determine the relationship between age, aerobic capacity (VO2 max) and other health indicators among 3 rescue groups. The type of training and the subjective perception of physical fitness obtained via the Assessment Questionnaire of Physical Fitness were also analysed. To obtain VO2 max, 37 firefighters, 22 lifeguards and 59 mine rescue workers had a treadmill test. Their body mass index and body fat percentage were also calculated. The results show a significant decline in VO2 max of the older participants, which affects the effectiveness of rescue work. Furthermore, the training of all groups was inconsistent and based on individual needs. Variable training and the decline in VO2 max with age affected the effectiveness of the rescue tasks of each group.

Vegetation and hydrogeology along the distribution range of Centaurium somedanum, an endemic plant of mountain calcareous springs

Calcareous springs support bryophyte-dominated habitats relevant to biodiversity and conservation, but not much is known about the ecology of vascular plants restricted to these environments. Here, we investigate vegetation and hydrogeological patterns within the distribution of Centaurium somedanum, a threatened vascular plant endemic to mountain calcareous springs in the Cantabrian range (Spain). We studied the hydrogeological characteristics of the spring systems where this species occurs and identified three types of springs: (a) springs with active travertine deposition in small valleys, (b) springs in rock fall avalanches and (c) springs fed by calcareous water close to geological faults. Vascular plants and bryophytes co-occurring with the study species were sampled in 1-m2 plots to identify major vegetation types and to assess the relationships between species composition and topography. The presence of C. somedanum was associated with vegetation of calcareous spring fens (Caricion davallianae) in geological faults, tufa concretions (Adiantion) in active travertines, and another vegetation type of calcareous flushes (Pinguiculion) that was found in all of the spring types. C. somedanum shows a wider ecological range than other vascular plants of calcareous springs, occupying micro-habitats with similar cover of bryophytes but different from the cover of vascular plants. Although these plant communities are primarily dependent on the hydrology of springs, the variation in species composition is also correlated to the slope and solar radiation at the micro-scale. Our study demonstrates how a vascular plant with a calciphilic character may adapt to tufa-forming niches along the spring-flush-fen gradient, thus allowing it to occupy a wide range of habitats at different altitudes and geohydrological conditions.

Systematics and management of natural resources: the case of Spartina species on European shores

Discrepancies in the identification of some plants and, in consequence on their autochthonous or allochthonous character, can lead to the adoption of inappropriate habit management strategies such as conservation, control, elimination, etc. A clear illustration of this is the case of a plant with an evident expansive behaviour located on the North Atlantic coast of the Iberian Peninsula, which has been considered by some authors as Spartina versicolor, a native of the European coasts. However, other authors have identified this plant as Spartina patens, originally from the North American Atlantic coasts, but introduced on both the Atlantic and Mediterranean coasts of Europe. This species shows an invading behaviour, playing a clear and evident role in the transformation of the habitats that it colonizes. In this work, results based on the use of sequences of the internal transcribed spacer (ITS) region of nrDNA, widely used in taxonomy and molecular phylogeny between closely related species, is reported. These data indicate that the identity of those plants growing on the European littoral is similar to those native to the North American Atlantic coasts.

Design and validation of a questionnaire to assess cooperative learning in educational contexts

Resumen: El objetivo del estudio fue elaborar y validar un instrumento que pudiera evaluar los elementos fundamentales del aprendizaje cooperativo, así como proporcionar un factor de cooperación. Participaron 11.202 estudiantes de educación primaria (5o-6o curso), secundaria y bachillerato (5.838 varones, 5.364 mujeres) de 68 centros educativos en 62 ciudades españolas repartidas por toda su geografía. Las edades oscilaron entre los 11 y los 18 años. El único requisito para participar era haber experimentado varias técnicas de aprendizaje cooperativo en los últimos 6 meses. Tras elaborar una primera versión y ser sometida sucesivamente a un juicio de expertos y un estudio piloto se realizó un segundo estudio en el que se sometió la versión definitiva a diferentes pruebas estadísticas. El Cuestionario de Aprendizaje Cooperativo está formado por cinco sub-escalas: Interacción Promotora, Interdependencia Positiva, Responsabilidad Individual, Procesamiento Grupal y Habilidades Sociales. Los análisis factoriales confirmatorios mostraron que todos los índices de fiabilidad eran aceptables, incluso bajo las condiciones más exigentes. El cuestionario mostró una adecuada validez convergente, discriminante y concurrente. Se confirma como un instrumento sencillo para evaluar todos los elementos fundamentales del aprendizaje cooperativo en estudiantes de primaria, secundaria y bachillerato y proporcionar un factor de cooperación global. Palabras clave: cooperación; primaria; secundaria; bachillerato. Title: Design and validation of a questionnaire to assess cooperative learning in educational contexts. Abstract: The goal of the present study was to design and validate an instrument to assess the basic elements of cooperative learning, as well as a cooperation index. 11.202 primary education (grades 5, 6), secondary education and baccalaureate students (5.838 males, 5.364 females) from 68 different schools in 62 cities all over Spain agreed to participate. The age range was 11-18 years. The participating students had experienced several cooperative learning techniques during the last six months. The first version of the questionnaire was assessed by a group of experts. A pilot study with 60 students similar to the target sample was conducted on the second version of the instrument. The final version underwent several statistical tests. The Cooperative Learning Questionnaire included five subscales: Promotive Interaction, Positive Interdependence, Individual Accountability, Group Processing and Interpersonal skills. Factorial and confirmatory analysis showed that all reliability indices were acceptable, even under the most difficult conditions. The questionnaire showed adequate convergent, discriminant and concurrent validity. Results showed that it is an easy instrument to assess all the basic elements of cooperative learning in primary, secondary and baccalaureate students and obtain a global cooperation factor.