José Carlos González

Role of maternal adrenal glands on the developing serotoninergic and aminoacidergic systems of the postnatal rat brain

Serotonin, γ‐aminobutyric acid and glutamate, which are regulated by glucocorticoids in the central nervous system, are involved in neuroendocrine functions and the development of the brain. The present study investigates the effect of maternal adrenalectomy on the developing serotoninergic, GABAergic and glutamatergic systems. Neurotransmitter levels were measured in four brain areas of both male and female offspring on postnatal days 1, 8, 12 and 22. At postnatal day 1 and 8, the pups of adrenalectomized dams showed higher concentrations of serotonin than controls in all the brain areas studied. Serotonin levels decreased significantly in males at postnatal day 22 in the hippocampus and cortex. During the first 2 weeks of postnatal life, the lack of maternal corticosterone produced an increase in glutamate and a reduction in γ‐aminobutyric acid concentrations, mainly in males. Further, on postnatal day 1, increased serotonin and glutamate levels and lower levels of γ‐aminobutyric were observed in the hypothalamus of male pups born to adrenalectomized dams. The absence of maternal corticosterone affects the pattern of development of the serotoninergic system, especially in the hippocampus and cortex, and particularly in males. A delay in the maturation of the aminoacidergic systems, mainly of the GABAergic system and in males, was also seen. A sexually dimorphic response to the removal of maternal glucocorticoids was seen in terms of neurotransmitter levels, mainly in the hippocampus and hypothalamus.

Role of maternal corticosterone in the development and maturation of the aminoacidergic systems of the rat brain

Previous studies have suggested an important role for maternal glucocorticoids in the development of the aminoacidergic systems of the rat brain. This study examines the effect of metyrapone (2‐methyl‐1,2‐di‐3‐pyridyl‐1‐propanone), i.p.‐administered to gestating mothers, on the maturation of the aminoacidergic systems of their offsprings brains. γ‐Aminobutyric acid (GABA) and glutamate concentrations were determined in male and female offspring at postnatal days (PN) 23 and 90 in four brain areas: the hippocampus, hypothalamus, striatum and cortex. The activity of glutamic acid decarboxylase (GAD), the most important enzyme in the synthesis of GABA, was also analysed. The results show that a reduction in maternal corticosterone during gestation leads to a reduced GABAergic content in all brain areas studied at PN23; permanent organizational changes occurred in the cortex, striatum and hypothalamus. Maternal metyrapone treatment also affected the development of the glutamatergic systems, females being more affected than males at both PN23 and PN90 particularly in the hypothalamus and cortex. The metyrapone treatment produced no changes in GAD activity at PN23, but induced an important increase in this activity at PN90.

A three-layer planning architecture for the autonomous control of rehabilitation therapies based on social robots ☆

Abstract This manuscript focuses on the description of a novel cognitive architecture called NAOTherapist, which provides a social robot with enough autonomy to carry out a non-contact upper limb rehabilitation therapy for patients with physical impairments, such as cerebral palsy and obstetric brachial plexus palsy. NAOTherapist comprises three levels of Automated Planning. In the high-level planning, the physician establishes the parameters of the therapy such as the scheduling of the sessions, the therapeutic objectives to be achieved and certain constraints based on the medical records of the patient. This information is used to establish a customized therapy plan. The objective of the medium-level planning is to execute and monitor every previous planned session with the humanoid robot. Finally, the low-level planning involves the execution of path-planning actions by the robot to carry out different low-level instructions such as performing poses. The technical evaluation shows an accurate definition and monitoring of the therapies and sessions and a fluent interaction with the robot. This automated process is expected to save time for the professionals while guaranteeing the medical criteria.

Evaluating the Child–Robot Interaction of the NAOTherapist Platform in Pediatric Rehabilitation

NAOTherapist is a cognitive robotic architecture whose main goal is to develop non-contact upper-limb rehabilitation sessions autonomously with a social robot for patients with physical impairments. In order to achieve a fluent interaction and an active engagement with the patients, the system should be able to adapt by itself in accordance with the perceived environment. In this paper, we describe the interaction mechanisms that are necessary to supervise and help the patient to carry out the prescribed exercises correctly. We also provide an evaluation focused on the child-robot interaction of the robotic platform with a large number of schoolchildren and the experience of a first contact with three pediatric rehabilitation patients. The results presented are obtained through questionnaires, video analysis and system logs, and have proven to be consistent with the hypotheses proposed in this work.

Effects of maternal cadmium administration on development of monoaminergic, GABAergic and glutamatergic systems

The effects of maternal exposure to 10mgCd/l (as cadmium acetate) in drinking water during gestation and lactation on the development of monoaminergic and aminoacidergic systems were studied in discrete brain areas of the pups: striatum, cerebral cortex, dorsal hippocampus and basal-medial hypothalamus. Hippocampal levels of serotonin and 5-hydroxyindolacetic acid were significantly reduced in rats exposed to Cd whereas the dopamine content was not significantly affected by Cd. Glutamate concentration decreased in hypothalamus and increased in hippocampus, while gamma-aminobutiric acid content decreased only in cerebral cortex. The present results demonstrate that maternal exposure to 10mg/l of Cd leads to neurochemical disturbances on serotoninergic and aminoacidergic systems during development.

Influence of metyrapone treatment during pregnancy on the development and maturation of brain monoaminergic systems in the rat

Aim:u2002 This study examines the effect of reducing the corticosterone levels of gestating rat dams on the postnatal development and maturation of monoaminergic systems in their offspring’s brains.

Therapy Monitoring and Patient Evaluation with Social Robots

Social robots have a great potential. With high movement capabilities and large computational capacity, they allow to perform varied tasks that were usually conducted by humans. One of these tasks are physical therapies, where a therapist guides a patient through the realisation of a set of exercises. A robot, equipped with a sophisticated artificial vision system, can conduct these therapies and evaluate the patient movements. In this paper, we present a system that allows the therapist to design a complete therapy to be carried out by the robot, to start each session with the robot, to evaluate the patient condition over the therapy and to generate reports at the end of a session.

CLARC: A Cognitive Robot for Helping Geriatric Doctors in Real Scenarios

Comprehensive Geriatric Assessment (CGA) is an integrated clinical process to evaluate the frailty of elderly persons in order to create therapy plans that improve their quality of life. For robotizing these tests, we are designing and developing CLARC, a mobile robot able to help the physician to capture and manage data during the CGA procedures, mainly by autonomously conducting a set of predefined evaluation tests. Built around a shared internal representation of the outer world, the architecture is composed of software modules able to plan and generate a stream of actions, to execute actions emanated from the representation or to update this by including/removing items at different abstraction levels. Percepts, actions and intentions coming from all software modules are grounded within this unique representation. This allows the robot to react to unexpected events and to modify the course of action according to the dynamics of a scenario built around the interaction with the patient. The paper describes the architecture of the system as well as the preliminary user studies and evaluation to gather new user requirements.