José Carlos Ribeiro Júnior

Complete Genome Sequence of Streptococcus agalactiae Strain S25 Isolated from Peritoneal Liquid of Nile Tilapia

ABSTRACT Streptococcus agalactiae (Lancefield group B; GBS) is one of the major pathogens in fish production, especially in Nile tilapia (Oreochromis niloticus). The genomic characteristics of GBS isolated from fish must be more explored. Thus, we present here the genome of GBS S25, isolated from Nile tilapia from Brazil.

Avaliação da qualidade microbiológica e físicoquímica do leite cru refrigerado produzido na região de Ivaiporã, Paraná

The aim of this work was to evaluate the microbiological and physico-chemical parameters of refrigerated raw milk produced in 99 dairy farms from Ivaipora-PR region, from August to October 2010. Samples out of standards for that period of time were observed to all analysis determined by legislation: alizarol 72% v/v (63,38%), titratable acidity (54,05%), crioscopy (8,10%), density (5,4%), fat (17,17%), total protein (14,14%), non-fat solids (26,26%), SCC (17,17%) and TBC (54,08%). Frauds by water addition and skim of milk were observed. Results suggest de cient milking hygiene and lack of sanity control of mammary gland, showing that the legislation goals for milk quality improvement initiated at 2002 are not being achieved by the producers.The aim of this work was to evaluate the microbiological and physico-chemical parameters of refrigerated raw milk produced in 99 dairy farms from Ivaipora-PR region, from August to October 2010. Samples out of standards for that period of time were observed to all analysis determined by legislation: alizarol 72% v/v (63,38%), titratable acidity (54,05%), crioscopy (8,10%), density (5,4%), fat (17,17%), total protein (14,14%), non-fat solids (26,26%), SCC (17,17%) and TBC (54,08%). Frauds by water addition and skim of milk were observed. Results suggest deficient milking hygiene and lack of sanity control of mammary gland, showing that the legislation goals for milk quality improvement initiated at 2002 are not being achieved by the producers.

AVALIAÇÃO DA QUALIDADE FÍSICO-QUÍMICA E MICROBIOLÓGICA DE CREMES DE LEITE UHT

UHT products consumption has increased in Brazil, due to its practical storage and long shelf life. The aim of the present work was to verify physico-chemical and microbiological quality of UHT milk cream, commercialized in Londrina-PR. A total of 14 brands were evaluated, three samples for each brand were collected, toting up 42 samples. Regarding fat content, 26,19% of samples did not present the percentage described on the carton of the product, and the other 73,81% presented the expected percentage of label or superior values. As for acidity analysis, none of the results found had values superior than standard established by law. For mesophilic aerobes count, only one sample (2,38%) presented counts higher than standard of 100CFU/g of milk cream established by MAPA (Brazilian Ministry of Agriculture Livestock and Supply, that inspects products at industry). On the other hand, 73,80% were out of standard according with ANVISA (Brazilian National Agency for Sanitary Surveillance, which establishes standards for food safety assurance at the market). The remaining samples (23,80%) did not present any growth. From 110 isolated colonies of BHI agar and nutrient agar, all were Gram positives from which 48,20% were bacillus, 41,8% were cocci, 7,3% with suggested yeast morphology, 1,8% were coccobacillus and 0,9%were diplococci. The most frequent problem evidenced by the present work was fat content lower than described on carton. Microbiological standards established for UHT milk cream by different government institutions are ambiguous and difficult product assessment. Using both standards, the majority of samples presented satisfactory quality according with industry legislation and, at the same time, were out of standards for the market.

IMPACTO DA IMPLANTAÇÃO DE BOAS PRÁTICAS DE HIGIENE NA ORDENHA SOBRE A QUALIDADE MICROBIOLÓGICA E FÍSICO-QUÍMICA DO LEITE CRU REFRIGERADO

Normative instruction no 62 brought new standards to be followed by producers, proceeding to continue the improvement of milk quality in progress since Normative instruction no 51. However quality improvement of milk did not occur as fast as expected. The aim of this work was to evaluate the impact of good practices on milk quality in 49 dairy farms located in Ivaipora/PR, through collective lectures and training courses. A reduction of 88,05% in Total Bacterial Count (TBC) in 28 (57,15%) dairy farms that implemented the good practices was observed. From those farms, 23 (47,93%) presented TBC bellow maximum of 750.000 CFU/mL as required by legislation at the period of analysis, with average counts of 166.826,1 CFU/mL. As for Somatic Cell Counts (SCC), 83,57% of all farms began to produce milk according with the standard set to this item at the time of analysis. Significant variations were not observed in physico-chemical parameters of milk after practices implementation. Implementation of the proposed practices was sufficient to produce milk according with standards established by legislation.