José Dias de Souza Filho
Universidade Federal de Minas Gerais
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by José Dias de Souza Filho.
Journal of Ethnopharmacology | 2013
Leidiane C. Ferreira; Andrea Grabe-Guimarães; Carmen Aparecida de Paula; Marcela Carolina de Paula Michel; Raquel G. Guimarães; Simone Aparecida Rezende; José Dias de Souza Filho; Dênia Antunes Saúde-Guimarães
ETHNOPHARMACOLOGICAL RELEVANCE Campomanesia species are used in folk medicine as anti-inflammatory, anti-rheumatic, anti-diarrheal and hypocholesterolemic. AIM OF THE STUDY The present study investigated the in vivo anti-inflammatory and antinociceptive properties of ethyl acetate (AE) and aqueous (Aq) extracts from leaves of Campomanesia adamantium and in vitro anti-inflammatory activity of AE and its isolated flavonols, myricitrin and myricetin. MATERIALS AND METHODS The antinociceptive activity of AE and Aq was evaluated using acetic acid-induced writhing and formalin methods. The in vivo anti-inflammatory effect of AE and Aq was evaluated using carrageenan-induced paw oedema in mice. AE, myricitrin and myricetin were evaluated for their abilities to modulate the production of NO, TNF-α and IL-10 in LPS/IFN-γ stimulated J774.A1 macrophages. RESULTS It was found that orally administrated AE and Aq (125 and 250 mg/kg) inhibited carrageenan-induced paw oedema in mice. AE (125 and 250 mg/kg) and Aq (125 mg/kg) reduced the time to licking at the second phase of the formalin method in vivo in mice. AE (250 mg/kg) and Aq (125 mg/kg) also reduced the number of writhes. AE, myricitrin and myricetin inhibited NO (320 μg/mL and 6.25-100 μM, respectively) and TNF-α production by macrophages (320 μg/mL for AE, 100 μM for myricitrin and 25-100 μM for myricetin). AE (160 and 320 μg/mL), myricitrin (50 and 100 μM) and myricetin (25-100 μM) increased IL-10 production by macrophages. CONCLUSIONS The ethyl acetate and aqueous extracts from Campomanesia adamantium showed antinociceptive and anti-inflammatory effects supporting the use of the plant in folk medicine. The results suggest that anti-oedematogenic effect promoted by aqueous extract involves several anti-inflammatory mechanisms of action. The antinociceptive effect shown by aqueous extract can be due to the modulation of release of inflammatory mediators involved in nociception. The anti-inflammatory effects of AE and of its isolated flavonols may be attributed to inhibition of pro-inflammatory cytokines production, TNF-α and NO and to the increased of IL-10 production.
European Journal of Medicinal Chemistry | 2012
Eufranio N. da Silva Junior; Isadora M.M. de Melo; Emilay B. T. Diogo; Verenice A. Costa; José Dias de Souza Filho; Wagner O. Valença; Celso A. Camara; Ronaldo N. de Oliveira; Alexandre Suman de Araujo; Flavio da Silva Emery; Marcelo R. dos Santos; Carlos A. de Simone; Rubem F. S. Menna-Barreto; Solange L. de Castro
Five 2-hydroxy-3-substituted-aminomethyl naphthoquinones, nine 1,2,3-triazolic para-naphthoquinones, five nor-β-lapachone-based 1,2,3-triazoles, and several other naphthoquinonoid compounds were synthesized and evaluated against the infective bloodstream form of Trypanosoma cruzi, the etiological agent of Chagas disease, continuing our screening program for new trypanocidal compounds. Among all the substances, 16-18, 23, 25-29 and 30-33 were herein described for the first time and fifteen substances were identified as more potent than the standard drug benznidazole, with IC(50)/24h values in the range of 10.9-101.5 μM. Compounds 14 and 19 with Selectivity Index of 18.9 and 6.1 are important structures for further studies.
Phytochemistry | 2003
Elita Scio; Antônia Ribeiro; Tânia M. A. Alves; Alvaro J. Romanha; José Dias de Souza Filho; Geoffrey A. Cordell; Carlos L. Zani
Further investigation of the aerial parts of Alomia myriadenia revealed an halimane diterpene identified as ent-8S,12S-epoxy-7R,16-dihydroxyhalima-5(10),13-dien-15,16-olide along with the known ent-16-hydroxylabda-7,13-dien-15,16-olide, ent-12R-hydroxylabda-7,13-dien-15,16-olide, 6,7-methylenedioxycoumarin (ayapin), and kaempferol-7-methylether (rhamnocitrin). Evaluated in a panel of human cancer cell lines, the 16-hydroxylabade diterpene was the most active, showing an ED(50) value of 0.3 mug/ml against Lu1 (human lung cancer) cells. Tested in vitro against Trypanosoma cruzi in infected murine blood, this compound caused lysis of 100% of the parasites at 250 mug/ml.
Journal of Ethnopharmacology | 2012
Maíra Ribeiro de Souza; Carmen Aparecida de Paula; Michelle Luciane Pereira de Resende; Andrea Grabe-Guimarães; José Dias de Souza Filho; Dênia Antunes Saúde-Guimarães
ETHNOPHARMACOLOGICAL RELEVANCE The ethanolic extract of Lychnophora trichocarpha Spreng. is used in Brazilian folk medicine to treat bruise, pain and inflammatory diseases. AIM OF THE STUDY The present study aimed at investigating whether ethanolic extract of L. trichocarpha, its ethyl acetate fraction and its main bioactive compounds could be useful to treat gouty arthritis by countering hyperuricemia and inflammation. MATERIALS AND METHODS L. trichocarpha ethanolic extract (LTE), ethyl acetate fraction from ethanolic extract (LTA) and isolated compounds were evaluated for urate-lowering activity and liver xanthine oxidase (XOD) inhibition in oxonate-induced hyperuricemic mice. Anti-inflammatory activity in monosodium urate crystal-induced paw oedema, an experimental model of gouty arthritis, was also investigated. RESULTS Crude ethanolic extract and its ethyl acetate fraction showed significant urate-lowering effects. LTE was also able to significantly inhibit liver xantine oxidase (XOD) activity in vivo at the dose of 250mg/kg. Luteolin, apigenin, lupeol, lychnopholide and eremantholide C showed the anti-hyperuricemic activities among tested compounds. Apigenin also showed XOD inhibitory activity in vivo. Luteolin, lychnopholide, lupeol and eremantholide C, in turn, did not shown significant inhibitory activity towards this enzyme, indicating that this mechanism is not likely to be involved in urate-lowering effects of those compounds. LTE, LTA, lupeol, β-sitosterol, lychnopholide, eremantholide, luteolin and apigenin were also found to inhibit monosodium urate crystals-induced paw oedema in mice. CONCLUSIONS Ethanolic extract of Lychnophora trichocarpha and some of its bioactive compounds may be promising agents for the treatment of gouty arthritis since they possesses both anti-hiperuricemic and anti-inflammatory properties.
Magnetic Resonance in Chemistry | 2008
Priscilla B. M. C. Derogis; Felipe T. Martins; Thiago Corrêa de Souza; Maria Eliza de Castro Moreira; José Dias de Souza Filho; Antonio C. Doriguetto; Kamila R. D. de Souza; Marcia Paranho Veloso; Marcelo Henrique dos Santos
This article reports the structural elucidation by IR, UV and MS spectroscopic data along with 1H and 13C NMR chemical shift assignments of two benzophenones isolated from the fruit pericarp of Garcinia brasiliensis Mart. (Clusiaceae): garciniaphenone, (1R,5S,7S)‐3‐benzoyl‐4‐hydroxy‐6,6‐dimethyl‐5,7‐di(3‐methyl‐2‐butenyl)bicyclo[3.3.1]non‐3‐ene‐2,9‐dione, a novel triprenylated benzophenone; and 7‐epi‐clusianone, a tetraprenylated benzophenone that has already been extracted from another species of the same family. Furthermore, the keto‐enol tautomeric equilibrium at solution‐state was described for these compounds by 1D and 2D NMR spectral methods and one attempt to rationalize the different ratios between the noted tautomers was based on stereochemical features. Copyright
European Journal of Medicinal Chemistry | 2009
Raphael S. F. Silva; Maria do Carmo F. R. Pinto; Marília Oliveira Fonseca Goulart; José Dias de Souza Filho; Ivan Neves; Maria Cristina S. Lourenço; Antonio V. Pinto
We report here an alternative to the MCPBA or ozonolysis-based oxidation methods of quinoxaline-featuring compounds prepared from beta-lapachones. The use of peracetic acid allowed a simple preparation of the corresponding macrolactones by cleavage of the ring system. These lactones were evaluated for their antimycobacterial potential and compound 4 turned out to have an MIC of 0.62 microg per mL on Mycocabteriumtuberculosis H37Rv. These results justify further research into its value as a potential lead for an original treatment of tuberculosis.
Phytomedicine | 2012
Patricia Baier Krepsky; Raquel Geralda Isidório; José Dias de Souza Filho; Steyner F. Cortes; Fernão Castro Braga
The aerial parts of Cuphea carthagenensis (Jacq.) J.F. Macbride (Lythraceae) are traditionally employed in Brazil to treat cardiovascular diseases. The aim of this study was to compare preparations of C. carthagenensis aerial parts (aqueous and ethanol extracts, together with derived fractions) with regard to their total phenolic contents and in vitro vasodilating activity. The main flavonoids found in the extracts were isolated and identified as quercetin derivatives. The extracts and fractions showed similar HPLC profiles with the presence of quercetin-5-O-β-glucopyranoside, quercetin-3-O-α-arabinofuranoside and quercetin-3-sulfate in all of them, but marked differences in the contents of flavonoids, proanthocyanidins, tannis and total phenolics. Excepting the aqueous extract, all assayed preparations elicited vasodilatation on pre-contracted rat aortic rings in the range of pIC(50) 4.53±0.03 to 4.98±0.06. Polynomial regression analysis demonstrated the relationship between vasodilating activity and the contents of flavonoids (r(2)=0.5190), proanthocyanidins (r(2)=0.8016), tannins (r(2)=0.8041) and total phenolics (r(2)=0.6226), suggesting the participation of these compounds in the pharmacological effect and their potential use as chemical markers for the species.
Evidence-based Complementary and Alternative Medicine | 2013
Paula S. Lage; Pedro Henrique Rocha de Andrade; Amanda de Santana Lopes; Miguel Angel Chávez Fumagalli; Diogo G. Valadares; Mariana C. Duarte; Daniela P. Lage; Lourena E. Costa; Vívian T. Martins; Tatiana G. Ribeiro; José Dias de Souza Filho; Carlos Alberto Pereira Tavares; Rodrigo Maia de Pádua; João Paulo Viana Leite; Eduardo Antonio Ferraz Coelho
The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivity-guided fractionation was performed, and two flavonoids were identified, quercetin 3-O-methyl ether and strychnobiflavone, which presented an effective antileishmanial activity against L. amazonensis, and studies were extended to establish their minimum inhibitory concentrations (IC50), their leishmanicidal effects on the intra-macrophage Leishmania stage, as well as their cytotoxic effects on murine macrophages (CC50), and in O+ human red blood cells. The data presented in this study showed the potential of an ethyl acetate extract of S. pseudoquina, as well as two flavonoids purified from it, which can be used as a therapeutic alternative on its own, or in association with other drugs, to treat disease evoked by L. amazonensis.
Memorias Do Instituto Oswaldo Cruz | 2003
Renata Barbosa de Oliveira; Ana Paula F. Passos; Rosana O. Alves; Alvaro J. Romanha; Maria Auxiliadora Fontes Prado; José Dias de Souza Filho; Ricardo José Alves
Fourteen compounds were evaluated for their activity against Trypanosoma cruzi blood stream forms at the concentration of 500 g/ml. Six compounds were active and re-tested at lower concentrations.
Journal of the Brazilian Chemical Society | 2001
Brás H. de Oliveira; Tomoe Nakashima; José Dias de Souza Filho; Fabiano L. Frehse
Neste trabalho sao descritos o isolamento e a identificacao de eupafolina e hispidulina das folhas secas de Eupatorium littorale (Asteraceae). Foi desenvolvido tambem um metodo para a deteccao desses flavonoides e de rutina na planta, utilizando a tecnica de CLAE em fase reversa com detetor de arranjo de fotodiodos. Eupafolina e hispidulina foram quantificados sendo encontradas as concentracoes de 0,29% e 0,21%, respectivamente.