José Henry Osorio

Ovarian response of goats submitted to implants of progesterone followed by administration of equine corionic gonadotrophin

The objective of the present study was to compare the effect of different dosages of equine chorionic gonadotrophin (eCG) on the follicular dynamics and hormonal concentrations in Alpine goats. During the breeding season, the goats were submitted to estrous and ovulation synchronization with a device containing progesterone for 14 days. Female goats (n=24) were divided at random into four groups of 6 animals each. On the day of dispositive removal, the animals received 0 (Control), 200, 300 and 400 UI of eCG. Follicular development was observed by ultrasonography one day prior to administration of eCG until next ovulation. The plasma estradiol (E2) and progesterone (P4) concentrations were daily determined using RIA. All treated animals showed estrous. Estrous cycle with three and four waves of follicular growth were observed. Size of the largest follicle was smaller in control group animals of the third wave (5.5 ± 0.50 mm) when compared to those treated with 300 UI of eCG (7.17 ± 0.35 mm). Gonadotrophin increased the number of small and medium follicles considering the control group because the average number of corpora lutea were higher in 400 UI (4.27 ± 0.23) females than those treated with 200 UI (1.95 ± 0.19). Plasma concentrations of progesterone and stradiol showed differences among experimental groups. Combinations among progesterones and eCG is an adequate alternative for estrus synchronization and support the development of new protocols for reproduction techniques as superovulation and embryo transfer in goats.

Efeito do tempo da aplicação de prostaglandina na resposta folicular em ovelhas durante o ciclo estral

The purpose of this study was to evaluate ovarian response, using transrectal ultrasonography, to two injections of Prostaglandin F2α (PGF2α) given at different intervals. Fourteen Bergamasca ewes were injected with an i.m. dose of PGF2α during the breeding season. Ewes were randomly alloted in two groups (n = 7) which received PGF2α 7 d (PG7) or 9 d (PG9) after ovulation (when the dominant follicle of wave 2 was expected to be in the growth and plateau phase, respectively). Ovaries were examined daily with transrectal ultrasonography Aloka 500 with 7.5 MHz linear array transducer. Ultrasonography started 1 day prior to the second PG injection and ended 10 d after ovulation for both groups. Jugular vein blood samples were collected daily for progesterone assay. Plasma P4 concentration was lower in PG7 than in PG9, immediately before PGF2α treatment (4.10 ± 0.17 vs 4.91 ± 0.29 ng/mL, respectively). Mean maximum diameter size attained by the dominant follicle of wave 2 was larger in the group treated in PG7 when compared with PG9 (5.5 ± 0.19 vs 4.29 ± 0.26 mm). There was effect of day for total follicles. Number of small follicles (2-2.5 mm) was greater (4.57 ± 0.78 vs 8.42 ± 1.36) at day 6, for PG7 and PG9, respectively, whereas no changes were observed in medium follicles (3-3.5 mm) between groups treated. The proportion of large follicles (4 mm) increased after luteolysis. Values were lower for ewes treated on PG7, whereas values mantained stable over time for ewes treated on PG9. Ewes with rapid and complete luteal regression, ovulation occur from the dominant follicle of wave 2 in females treated on days 7 and 9.

Modelo de simulación y minería de datos para identificar y predecir cambios presupuestales en la atención de pacientes con hipertensión arterial

Objective To present a simulation model that establishes the economic impact to the health care system produced by the diagnostic evolution of patients suffering from arterial hypertension. Methodology The information used corresponds to that available in Individual Health Records (RIPs, in Spanish). A statistical characterization was carried out and a model for matrix storage in MATLAB was proposed. Data mining was used to create predictors. Finally, a simulation environment was built to determine the economic cost of diagnostic evolution. Results 5.7 % of the population progresses from the diagnosis, and the cost overrun associated with it is 43.2 %. Conclusions Results shows the applicability and possibility of focussing research on establishing diagnosis relationships using all the information reported in the RIPS in order to create econometric indicators that can determine which diagnostic evolutions are most relevant to budget allocation.

Comparación entre el Método Directo y la Fórmula de Friedewald para Determinar los Niveles de Colesterol LDL en Caninos

The aim of this study was to compare the Direct method and the Friedewald formula method for the determination of LDL cholesterol in dogs. Blood samples were collected in 185 fasted dogs without sex restriction. Serum was extracted and levels of LDL cholesterol were determined by the two methods. The mean, standard deviation, and minimum and maximum values by the Direct method were 49.2 ± 16.7, 14.6 and 96.0 mg/dl, respectively, and by the Friedewald method were 65.2 ± 31.5, 4.6 and 184.2 mg/dl, respectively, with significant difference between the methods (p<0.05). The Direct method is recommended for determining LDL cholesterol levels in dogs.

Comparación del método precipitado con polivinil sulfato y directo con Detergentes + DSBmT para la determinación del colesterol LDL en gallinas ponedoras

Summary The objective of this study was to compare two methods (precipitate vs. direct) to quantify low-density lipoprotein cholesterol in laying hens. Materials and methods: Cholesterol in low density lipoproteins was quantified using the precipitate (polyvinyl sulfate) method and the direct method based on detergent and N,Nbis (4-sulfobutyl )-m-toluidine. A total of 40 laying hens (Hy -Line W-36) aged 26 weeks were used. Results: Cholesterol levels (mg/dL) assessed by the precipitate and direct methods were 117.3 ± 38.6 and 49.3 ± 11.1, respectively. The P value for the F test was less than 0.05, showing statistically significant difference between methods, with a 95% confidence. Conclusions: The direct method based on detergents and N,Nbis(4-sulfobutyl)-m-toluidine can be used to determine cholesterol in low density lipoproteins of laying hens since it is not affected by serum triglyceride levels.