José Luis Benito Alonso

Recent Plant Diversity Changes on Europe’s Mountain Summits

Climb Every Mountain Mountaintop floras across Europe appear to be responding to climatic change in terms of upslope species range shifts. Pauli et al. (p. 353) systematically analyzed data gathered from standardized permanent plots on 66 high-mountain environments across Europe. On average, mountaintop species numbers have increased significantly during the last decade. However, this increase is a net effect of gains and losses, with losses particularly affecting mountains of Mediterranean regions and their endemic species. This turnover is largely consistent with model predictions and indicates that high-altitude species, and in particular the rich endemic alpine flora of many Mediterranean mountain ranges, will come under increasing pressure in the predicted warmer and drier climates in this region. European mountaintop flower species richness is increasing on northern summits but decreasing on southern summits. In mountainous regions, climate warming is expected to shift species’ ranges to higher altitudes. Evidence for such shifts is still mostly from revisitations of historical sites. We present recent (2001 to 2008) changes in vascular plant species richness observed in a standardized monitoring network across Europe’s major mountain ranges. Species have moved upslope on average. However, these shifts had opposite effects on the summit floras’ species richness in boreal-temperate mountain regions (+3.9 species on average) and Mediterranean mountain regions (–1.4 species), probably because recent climatic trends have decreased the availability of water in the European south. Because Mediterranean mountains are particularly rich in endemic species, a continuation of these trends might shrink the European mountain flora, despite an average increase in summit species richness across the region.

Differential effects of tumor necrosis factor on the growth and differentiation of neuroblastoma and glioma cells

We have studied the effect of tumor necrosis factor (TNF-alpha) on transformed neural and glial-derived cell lines. TNF-alpha at physiological doses was able to arrest the growth and inhibit DNA synthesis of N103 neuroblastoma cells. This phenomenon was accompanied by a morphological cell differentiation characterized by the outgrowth of neurites. By contrast, TNF-alpha induced an increase in the growth rate of C6 glioma cells and upon cytokine addition a higher number of C6 cells were found in the S + G2 phase of the cell cycle. C6 cells did not show morphological changes under this treatment. Analogous results were obtained with IFN-gamma. These neurotrophic and mitogenic effects of TNF-alpha suggest a putative role of this cytokine in the regeneration of brain tissue upon brain injury.

Regioselectivity of the enzymatic transgalactosidation of d- and l-xylose catalysed by β-galactosidases

The regioselectivity of enzymatic transgalactosidation depends on the source of the beta-galactosidase used. When the galactosyl acceptor only contains secondary hydroxyl groups, e.g., D- or L-xylose, it is possible to find an enzyme that catalyses preferentially the synthesis of any of the three regioisomers 4-, 3- and 2-O-beta-D-galactopyranosyl-D-xylose (1, 2 and 3, respectively) or 4-, 3- and 2-O-beta-D-galactopyranosyl-L-xylose (4, 5 and 6, respectively). Enriched mixtures in 1, 2 or 3 were obtained using beta-galactosidases from Escherichia coli, bovine testes or Aspergillus oryzae, respectively, by transgalactosidation reaction of O-nitrophenyl-beta-D-galactopyranoside and D-xylose, and enriched mixtures in 4, 5 or 6 were obtained in a similar way using beta-galactosidases from Aspergillus oryzae, lamb small-intestine (intestinal lactase-phloridzin hydrolase) or Saccharomyces fragilis, respectively, using L-xylose as acceptor.

Final design and progress of WEAVE: the next generation wide-field spectroscopy facility for the William Herschel Telescope

We present the Final Design of the WEAVE next-generation spectroscopy facility for the William Herschel Telescope (WHT), together with a status update on the details of manufacturing, integration and the overall project schedule now that all the major fabrication contracts are in place. We also present a summary of the current planning behind the 5-year initial phase of survey operations. WEAVE will provide optical ground-based follow up of ground-based (LOFAR) and space-based (Gaia) surveys. WEAVE is a multi-object and multi-IFU facility utilizing a new 2-degree prime focus field of view at the WHT, with a buffered pick-and-place positioner system hosting 1000 multi-object (MOS) fibres, 20 integral field units, or a single large IFU for each observation. The fibres are fed to a single (dual-beam) spectrograph, with total of 16k spectral pixels, located within the WHT GHRIL enclosure on the telescope Nasmyth platform, supporting observations at R~5000 over the full 370-1000nm wavelength range in a single exposure, or a high resolution mode with limited coverage in each arm at R~20000. The project is now in the manufacturing and integration phase with first light expected for early of 2018.

Integrando escalas y métodos LTER para comprender la dinámica global de un espacio protegido de montaña: el Parque Nacional de Ordesa y Monte Perdido

Bonache, J., de Mingo-Sancho, G., Serrada, J., Amengual, P., Perales, J., Martinez, R., Rodado, S., Albornos, E. 2016. Long-term monitoring and evaluation in Spanish National Parks Network. Ecosistemas 25(1): 31-48. Doi.: 10.7818/ECOS.2016.25-1.05 The Spanish National Parks Network, consisting of a selection of natural spaces containing a representative sample of the main existing natural systems in Spain, is a very appropriate setting for research and long-term monitoring, in which the rules protects and promotes the improvement of knowledge and its application to management. In this regard, the National Parks Agency develops, in collaboration with the Autonomous Communities and with specific Scientific advise, a Research Program and a Monitoring and Evaluation Plan of the National Parks Network, latter having programs of ecological, sociological monitoring and functional. The article briefly explains the various monitoring initiatives underway, with examples of outcomes in each: mapping of natural systems, productivity monitoring through remote sensing, monitoring plant health, common bird monitoring, studies of social perception etc. Future prospects and areas for improvement of long-term monitoring in the National Parks Network are explained taking into account the synergies with the LTER Network, which is useful for monitoring in National Parks, especially concerning the application of comparable protocols and information management.