José Luis Gama-Flores

Effect of Cadmium Level and Exposure Time on the Competition between Zooplankton Species Moina macrocopa (Cladocera) and Brachionus calyciflorus (Rotifera)

Competition among zooplankton is a natural phenomenon and often cladocerans are competitively superior to rotifers. However, anthropogenic factors including the release of industrial effluents, may influence this interaction. In this study, we evaluated the effect of cadmium (0.05 and 0.1 mg L(-1) as CdCl(2)) on competition between the cladoceran Moina macrocopa and the rotifer Brachionus calyciflorus. Since the release of industrial effluents is generally pulsed, we also exposed the test zooplankton species at different exposure periods (3, 6, 12 and 24 h) to the heavy metal. Regardless of exposure time and the presence of competing species, an increase in concentration of Cd resulted in decreased population growth of M. macrocopa and B. calyciflorus. Regardless of presence of the competing species and Cd concentration, an increase in exposure period resulted in decreased population growth rates of both the zooplankton species. In mixed cultures, in general, M. macrocopa outcompeted B. calyciflorus and completely eliminated it under conditions of high toxicant concentrations and longer exposure time.

Acute and chronic toxicity of the pesticide methyl parathion to the rotifer Brachionus angularis (Rotifera) at different algal (Chlorella vulgaris) food densities

Methyl parathion is a commonly used insecticide in Mexico to eradicate insect pests. We evaluated the effects of this insecticide on rotifer B. angularis using both acute and chronic toxicity tests. Median lethal concentration (LC50) of methyl parathion for B. angularis for a 24-h bioassay in the presence and absence of an algal diet was derived. Elevated LC50 due to the survival of a greater number of test individuals in the presence of food was observed. Regardless of the toxicant concentration, population growth curves of the animals maintained at the low food level (0.75×106 cells ml-1) had a longer lag phase than those at the high food level (1.5×106 cells ml-1). Regardless of food level, an increase in the toxicant concentration in the medium resulted in decreased population growth. The lowest peak population density (50 ind. ml-1) was observed at the highest toxicant concentration and the lower food level. The highest population density (200 ind. ml-1) was observed in the controls at high food level. The rates of population increase per day (r) in the controls were higher (from 0.14 to 0.37 depending on the food level). Irrespective of food level, there was a decrease in the r values with increasing pesticide concentration in the medium. In order to detect the effect of population density on the growth rates in relation to the toxicant stress, we plotted the daily growth rate against initial density for the entire duration of the experiment. We observed the existence of a significantly inverse relation at all treatments except at the low food level and high toxicant concentrations (0.625 and 1.25 mg l-1). We discuss the role of algae in the toxicity of methyl parathion to zooplankton.

Interaction among copper toxicity, temperature and salinity on the population dynamics of Brachionus rotundiformis (Rotifera)

Heavy metals may interact with ecological factors such as temperature, food level and salinity, causing both mortality and reduced reproduction in organisms. Among different heavy metals, copper compounds are commonly used for eliminating algal blooms in aquaculture tanks. At certain concentrations, copper is toxic to rotifers. In the present work, we evaluated the combined effects of salt concentrations (2.5 and 5.0 g l−1 NaCl), copper levels (0, 0.03125, 0.0625, 0.125 and 0.25 mg l−1 as CuCl2) and two temperatures (20 and 25 °C) on the population growth of B. rotundiformis using Chlorella as the algal food (at 0.5 × 106 cells ml−1 for every 24 h). Regardless of salinity and temperature, copper at concentrations as low as 0.03 mg l−1 had an adverse effect on the population growth of rotifers and above 0.125 mg l−1, the populations did not grow. The effect of the toxicant on B. rotundiformis was more severe at 25° than at 20 °C at lower salinity. In general, we observed peak densities of rotifers around day 12 at 20 °C but 6–8 days earlier at 25 °C. Peak population densities of B. rotundiformis in the controls at the salinity of 2.5 g l−1 ranged from 90 to 180 ind. ml−1, depending on temperature; at a salinity of 5.0 g l−1, these were lower. The population growth rates, r, in our study varied from +0.31 to −0.12 depending on the test conditions. There was a significant impact of temperature, salinity and toxicity level on the population growth rate of B. rotundiformis. Our results suggested that even narrow changes in salinity could negatively influence the toxicity of heavy metal on the population growth rates of B. rotundiformis.

POPULATION GROWTH OF EUCHLANIS DILATATA (ROTIFERA): COMBINED EFFECTS OF METHYL PARATHION AND FOOD (CHLORELLA VULGARIS)

In the present work, the combined impact of four concentrations (0, 0.0625, 0.125, and 0.25 mg/L) of methyl parathion and three densities (0.5 × 106, 1.0 × 106, and 2.0 × 106 cells/mL) of the green alga Chlorella vulgaris on the population growth of Euchlanis dilatata was studied. In general, regardless of the food level, an increase in the concentration of methyl parathion resulted in a significant reduction of the maximal population density and rate of population increase. The population growth rate in the controls ranged from 0.248 to 0.298; rates were lower in the presence of the pesticide. At any toxicant concentration, rotifers fed higher algal density showed significantly higher population growth compared with those at lower food levels. An interaction between toxicant and food level was evident on the population growth of E. dilatata. Results have been discussed in light of the protective role of algal density on the toxic effects of insecticides on rotifers and the differences in susceptibility to toxicants between planktonic and littoral rotifers.

Combined effects of exposure time and copper toxicity on the demography of Moina macrocopa (Crustacea: Cladocera)

Cohort life table experiments were conducted on M. macrocopa using copper at three nominal concentrations (as CuSO4, 0.1, 0.2 and 0.4 mg L− 1, in addition to controls) for different periods of exposure (3 to 24 h). Age-specific survivorship of M. macrocopa decreased in relation to age of the cohort, concentration and duration of exposure to copper. Age-specific life expectancy curves also decreased with increasing age of the cohort. However, at low copper levels and shorter exposure time, there was an increase in life expectancy when the cohort was about 10 day old. Fecundity (m x ) was nearly regular in controls; however, at higher copper levels and longer duration of exposure, the offspring production was more oscillating. The offspring production completely ceased when continuously exposed to CuSO4 at 0.4 mg L− 1. Most of the measured demography variables significantly decreased with increasing concentration of Cu in the medium. The average lifespan and life expectancy at birth varied 2.9 to 9.6 and 2.4 to 9.1 days, respectively. Gross reproductive rate (GRR) varied from 31 to 89 offspring female− 1 lifespan− 1, while the survival-weighted net reproductive rates were nearly one-third of the GRR. Generation time of M. macrocopa varied from 5 to 7 days while the rate of population increase ranged from 0.36 to 0.84 per day. The results are discussed in relation to the sensitivity of M. macrocopa to copper toxicity and the necessity of amending the national water quality criteria in Mexico.

Prey (Brachionus calyciflorus and Brachionus havanaensis) exposed to heavy metals (Cu and Cd) for different durations and concentrations affect predator's (Asplanchna brightwellii) population growth

In this work, we evaluated the combined effects of heavy metal concentration (CuSO4 and CdCl2 separately, at 0.1, 0.2 and 0.4 mg/L) and exposure time (6 and 24 h) on the population growth of the predatory rotifer Asplanchna brightwellii (predator) via its prey (Brachionus calyciflorus or Brachionus havanaensis). At low Cu concentration, B. calyciflorus stimulated the population growth of its predator as compared to controls. However, under higher Cd concentration and longer exposure time, B. calyciflorus caused lower growth of A. brightwellii. B. havanaensis previously exposed to Cu led to reduced growth of its predator, especially under high metal concentration. Regardless of the treatments, the population growth rates (r) of A. brightwellii varied from 0.11 ± 0.02 to 0.45 ± 0.04 d− 1. The exposure time had significant influence on r of A. brightwellii via only the Cd-exposed prey B. havanaensis. However, regardless of the exposure time, B. calyciflorus exposed to Cu and B. havanaensis exposed to Cd stimulated the growth rate of the predator. In our study the predator was not directly exposed to heavy metals. Therefore, the toxicants acted in an indirect way on A. brightwellii, i. e., the predators growth was affected through the ingestion of metal-contaminated prey.

Temperature and age affect the life history characteristics and fatty acid profiles of Moina macrocopa (Cladocera).

Demographic responses and fatty acid profiles of Moina macrocopa were quantified under different temperature regimes (20°C, 25°C and 30°C and diurnally variable 20-30°C) and at fixed ration (10.65µgDWml(-1)) of Chlorella. Highest constant temperature (30°C) reduced the density of M. macrocopa. The cladocerans under the fluctuating temperature regime too had lower population growth (about 50% lower than that at constant 25°C). The survivorship of M. macrocopa was higher at 20°C than that at 25°C and 30°C or at variable temperature regime. Gross and net reproductive rates were higher at 25°C. At 20°C, neonates had the highest proportion (67%) of myristic, palmitic and stearic acids while the adults had the lowest (26%) proportion. For both adults and neonates, palmitoleic, linoleic and linolenic comprised of 15-35% of the total fatty acids. Higher percentage (19%) of linoleic acid was present in adults than neonates (7%). Adults had linolenic acid level which was 3-times higher than in neonates. Linoleic and linolenic fatty acids decreased with increasing temperature for neonates and adults from 20°C to 30°C. The demographic responses and fatty acid profiles of M. macrocopa were discussed in relation to level and mode of temperature exposure.

Four Transgenerational Demographic Performance of Moina macrocopa Exposed to Chronic Levels of Cadmium

In this study, we quantified intergenerational, demographic variability of Moina macrocopa subjected to cadmium stress. Exposure of M macrocopa to cadmium (0.2, 0.3, and 0.4 mg/L as CdCl2) through 4 consecutive generations revealed changes in demographic responses not only in survivorship variables but also in reproductive parameters. Long-term demographic responses varied differently, depending on the demographic trait and the concentration of heavy metal in the medium. With the exception of generation time, all life history traits were significantly and adversely influenced due to increase in Cd concentrations. The average life span of M macrocopa varied up to 40% depending on Cd level and the generation of exposure. The highest gross reproductive rates were recorded in controls, while the lowest (∼30% less) were recorded at the highest Cd level. Survival-weighted net reproductive rates were reduced by nearly 50% due to Cd toxicity. The rate of population increase per day of M macrocopa was also significantly affected (∼25%) by Cd as compared to controls. This cladoceran showed a dose–response to Cd toxicity with a significance in both magnitude and frequency of offspring production.