José Luis Ramírez

A diverse assemblage of Anacardiaceae from Oligocene sediments, Tepexi de Rodríguez, Puebla, Mexico

Among the plants collected from the Pie de Vaca Formation of the Oligocene, of Tepexi de Rodríguez, Puebla, México are five plants of Anacardiaceae, Haplorhus medranoensis, Rhus toxicodendron, Rhus sp., Comocladia intermedia, and Pistacia marquezii represented by their leaves and/or leaflets. The past and present diversity and geographic distribution of one of these genera, Rhus, demonstrate its capability to adapt and diversify in a wide variety of environments. Leaf architecture characters of this taxon overlap with those of other genera in the family, suggesting a high degree of phenotypic plasticity. The presence in the Pie de Vaca Formation of a type of Pistacia with leaf architecture characters similar to those of Asian plants further supports a long history of exchange between low-latitude North America and Asia. Links between low-latitude North and South America and the Caribbean are suggested by the presence of Comocladia and Haplorhus. Whereas Comocladia highlights the long history of regional endemics in the area, Haplorhus, today an endemic monotypic genus of Peru, suggests exchange mechanisms between North and South America. The morphologic characters of these taxa, and those of Pseudosmodingium (Anacardiaceae), some Rosaceae, Leguminosae, and Berberidaceae, suggest that the Pie de Vaca community was established and evolved in harsh environmental conditions. The Pie de Vaca flora thus provides significant new insights into the biogeographic relationships of the low latitude vegetation of North America.

Leaves of Berberidaceae (Berberis and Mahonia) from Oligocene sediments, near Tepexi de Rodrı́guez, Puebla.

From the Oligocene Los Ahuehuetes locality, near Tepexi de Rodríguez, Puebla, Mexico, five new plant species are described based on their leaf architecture. The presence of brochidodromous or acrodromous venation, and secondary veins forming angular (versus rounded) arcs, are well defined characters in the fossil material that relate it to Berberidaceae. Comparison with the leaves and leaflets of extant and fossil plants allow the recognition of one Mahonia and four Berberis new species. The lack of detailed information on leaf architecture in Berberidaceae limits the evaluation of the taxonomic relationships that can be suggested between fossil and extant plants. However, from a biogeographic point of view the presence of these new fossil plants supports the hypothesis of a North American origin of the Orientalis Groups of Mahonia, to which a lineage of Berberis may be added. Furthermore, two of the new species suggest the dispersal, some time during the Tertiary, of a lineage that today forms the Australis Group of Berberis from low latitude North America to South America. The movement of the Chortis Block is proposed as an alternative to explain the dispersal of a growing list of plants from north to south in the Americas. Only through future geological and palaeobotanical work can this hypothesis be corroborated.

Reconstruction of the Leaves of Two New Species of Pseudosmodingium (Anacardiaceae) from Oligocene Strata of Puebla, Mexico

Leaves of two new plants are reconstructed from their isolated leaflets collected from the Oligocene Los Ahuehuetes locality near Tepexi de Rodríguez in Puebla, Mexico. The leaves of Pseudosmodingium mirandae Ramírez‐Garduño et al. are compound imparipinnate with leaflets of variable morphology. The leaflets of five leaf morphotypes vary from narrow elliptic to lanceolate or lorate; they are symmetrical to slightly asymmetrical, with acute to attenuate apex, acute to cuneate base, and entire to serrate margin. Venation is simple pinnate craspedodromous, with secondary veins slightly curved near their base; secondary veins may dichotomize near the margin to become tertiary veins, and intersecondary veins are small and oblique to the secondary veins. A small number of leaflets assigned to Pseudosmodingium terrazasiae Ramírez‐Garduño et al. are distinguished from P. mirandae by the leaflet shape, length:width ratio, base shape, and apex angle. Morphological comparison of the fossil leaves with leaves of extant species of Anacardiaceae based on numerical analyses indicates a close similarity between P. mirandae and Pseudosmodingium multifolium Rose, while P. terrazasiae is more similar to Pseudosmodingium perniciosum (HBK) Engl. The presence of fossil species with extant relatives that are endemic to Mexico, along with previous reports, indicates that by the Oligocene, some lineages were already in place, although today they form part of the more xeric communities in southern North America.

IN VIVO STUDY OF CEREBRAL WHITE MATTER IN THE DOG USING DIFFUSION TENSOR TRACTOGRAPHY

Conventional magnetic resonance imaging (MRI) allows investigators and clinicians to observe the anatomy and injuries of the cerebral white matter (CWM) in dogs. However, dynamic images based on the diffusion tensor (DT) technique are required to assess fiber tract integrity of the CWM. Diffusion tensor tractography (DTT) produces a three-dimensional representation in which data are displayed on a colored map obtained from the anisotropy of water molecules in the CWM tracts. Fractional anisotropy (FA) is a value that measures changes in water diffusion, which can occur if the CWM tracts are displaced, disrupted, or infiltrated. The goal of this study was to determine the feasibility of DTT for in vivo examination of the normal appearance of CWM in dogs through visual and quantitative analysis of the most representative CWM tracts. Nine tractographies were performed on healthy dogs using a 3T MRI scanner. T1- and T2-weighted images and DTI were acquired at different planes. Using DTT, three-dimensional reconstructions were obtained. Fractional ansisotropy and apparent diffusion coefficient (ADC) values of the right and left corticospinal tracts, corpus callosum, cingulum, and right and left fronto-occipital fasciculus were determined. Tract reconstructions were similar in 8/9 healthy dogs. Values for FA and ADC were similar in all the dogs. In one dog, tract reconstructions were inhomogeneous; these were displaced because it had larger lateral ventricles. Findings indicated that DTT is a feasible technique for in vivo study of CWM in dogs and that it complements information from conventional MRI.