Josefa Diva Nogueira Diniz

Absorção de macronutrientes por explantes de bananeira in vitro

The absorption of the nutrients (N, P, K, Ca, Mg and S) by banana (Musa sp.) cv. Prata Ana explants on the basic medium of Murashige & Skoog (1962) supplemented with sucrose (30 g/L) and BAP (3.5 mg/L) were evaluated at 0, 10, 20, 30, 40, 50 and 60 days after inoculation. The seven treatments were arranged on a completely randomized design with three replicates. The sequence of nutrient absorption by the explants was K > N > Ca ³ P > Mg @ S. The P was the nutrient with the fastest absorption rate and at the 30th day the explants had already absorbed 75% of the P from the medium. The P absorption stopped by the 50th day. The S absorption stopped at the 50th day with 66% of it remaining in the medium. The results suggested a close relationship between these two nutrients. The highest rates of nutrient absorption were observed during the first 20 days of culture. The rhizome, pseudostem and leaves behaved differently to the extraction and accumulation of nutrients.

Multiplicação in vitro de plantas juvenis de mamoeiro

The seminiferous propagation of Carica papaya L. presents a mixture of genotypes with a strong influence on fruit production costs, quality and yield , prevailing due to the lack of efficient protocols for vegetative propagation . In this context , the tissue culture emerges as a promising alternative, because it can provide, to farmers, sex determination in seedlings, with a high quality standard and enough amounts to supply commercial demands in all seasons . This study aimed at developing an in vitro micropropagation protocol from young papaya material, in two experiments. In the first experiment, the explants were multiplied in vitro and the cytokinins BAP, 2iP and KIN were tested at the concentrations of 0.0 mg L -1 , 2.5 mg L -1 , 5.0 mg L -1 and 10.0 mg L -1 , with the best results observed for 2iP, especially at the 2.5 mg L -1 concentration. In the second one, the 2iP cytokinin was used at the concentrations of 0.0 mg L -1 , 1.0 mg L -1 , 2.0 mg L -1 and 4.0 mg L -1 , with and without GA 3 (0.0 mg L -1 and 1.0 mg L -1 ), with beneficial effects of GA 3 observed in the multiplication process, especially when combined with 1.0 mg L -1 of 2iP, with the best results for plants height, number of leaves and explants sprouting.

Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

The transition from heterotrophism to autotrophism in acclimatization is a delicate process for most species. The objective of this work was to evaluate combinations of regional substrates of the Brazilian Northeast in the acclimatization and morphological development of minirose ( Rosa chinensis ‘Minima’) seedlings. The experiment was carried out in a greenhouse using seedlings with 30 days of in vitro culture. The treatments were constituted by: 1) dry coconut powder; 2) 75% dry coconut powder + 25% rice husk; 3) 50% dry coconut powder + 50% rice husk; 4) 75% dry coconut powder + 25% charcoal rice husk; 5) 50% dry coconut powder + 50% carbonized rice husk; 6) 100% vermiculite; 7) 50% vermiculite + 50% dry coconut powder; 8) commercial substrate; 9) 75% sand + 25% dry coconut powder and 10) 50% sand + 50% dry coconut powder. After 21 days, the following characteristics were evaluated: survival percentage, leaf number, shoot height, dry matter of shoot and root and total dry matter. The regional materials tested showed a good choice of substrates to be used in the acclimatization of minirose seedlings. The vermiculite presented the worst results in the characteristics evaluated. Dry coconut powder as substrate should be used in mixtures with other materials to provide better acclimatization conditions. The use of 50% dry coconut powder + 50% rice husk provided a best development and growth of minirose seedlings ( Rosa chinensis ‘Minima’) than other tested substrates

Multiplicação e enraizamento in vitro de Minirosa

O trabalho teve como objetivo desenvolver uma metodologia para a micropropagacao da minirosa. Foram realizados dois experimentos utilizando explantes retirados de plantas mantidas in vitro. No primeiro experimento, para multiplicacao, os explantes foram inoculados em meio MS com BAP, CIN e TCT isoladamente, nas concentracoes de 0,00; 0,01; 0,10; 1,00 e 10,00 mg L-1 com 15 tratamentos e 20 explantes por tratamento. Verificou-se aos 80 dias, que o BAP na concentracao de 1,0 mg L-1 favoreceu a emissao de gemas e o maior peso fresco dos explantes. Para o enraizamento, foram utilizadas diferentes concentracoes da formulacao dos sais macronutrientes do meio MS (25; 50; 75 e 100%) combinado com diferentes concentracoes de AIB (0,0; 0,1 e 1,0 mg L-1), constituindo um fatorial 4 x 3, em delineamento inteiramente casualizado para os dois experimentos. Aos 60 dias o melhor desenvolvimento das plantas com raizes normais foi observado quando se utilizou o meio MS na sua concentracao normal (100%) sem AIB. Dessa forma, para a multiplicacao e enraizamento in vitro de minirosa, deve ser utilizado o meio MS com 1,0 mg L-1 de BAP e o meio MS com 100% dos sais sem hormonio, respectivamente.