Josencler L. Ribas Ferreira
Universidade Federal do Rio Grande do Sul
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Featured researches published by Josencler L. Ribas Ferreira.
Aquatic Toxicology | 2014
Josencler L. Ribas Ferreira; María Noelia Lonné; Thiago F.A. França; Naiana R. Maximilla; Thiago Henrique Lugokenski; Patrícia Gomes Costa; Gilberto Fillmann; Félix Alexandre Antunes Soares; Fernando R. de la Torre; José M. Monserrat
Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p<0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 μg/L, and induced an increase (p<0.05) in BaP accumulation in the cells after 3 and 4h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p<0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p>0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.
Comparative Biochemistry and Physiology C-toxicology & Pharmacology | 2008
José M. Monserrat; Juliane Ventura Lima; Josencler L. Ribas Ferreira; Daiane da Silva Acosta; Márcia Longaray Garcia; Patrícia Ramos; Tarsila Barros Moraes; Luciane Cougo dos Santos; Lílian Lund Amado
Lipoic acid (LA) has been reported as a potential therapeutic agent due its antioxidants proprieties. It was considered its effect in different organs (gills, brain, muscle and liver) of the fish Corydoras paleatus (Callychthyidae). LA (70 mg/kg of body mass) was added to a commercial fish diet, organisms being fed daily (1% body weight). Sixty animals (mean mass: 2.37+/-0.09 g) were placed randomly in aquariums and received (+LA) or not (-LA) lipoic acid enriched diet during four weeks. After, fish were killed and the brain, muscle, gills and liver were dissected. LA treatment reduced significantly (p<0.05) reactive oxygen species concentration in brain and increased (p<0.05) glutamate-cysteine ligase activity in brain and liver of the same experimental group. LA fed organisms showed higher (p<0.05) brain glutathione-S-transferase activity, indicating that LA improves the detoxification and antioxidant capacity face components that waste glutathione in phase II reactions. A conspicuous reduction of protein oxidation was observed in muscle and liver of +LA organisms, indicating that the treatment was also effective in reducing oxidative stress parameters.
Aquatic Toxicology | 2012
Roberta Socoowski Britto; Márcia Longaray Garcia; Alessandra Martins da Rocha; Juliana Artigas Flores; M. V. B. Pinheiro; José M. Monserrat; Josencler L. Ribas Ferreira
In consequence of their growing use and demand, the inevitable environmental presence of nanomaterials (NMs) has raised concerns about their potential deleterious effects to aquatic environments. The carbon NM fullerene (C₆₀), which forms colloidal aggregates in water, and its water-soluble derivative fullerol (C₆₀(OH)₁₈₋₂₂), which possesses antioxidant properties, are known to be photo-excited by ultraviolet (UV) or visible light. To investigate their potential hazards to aquatic organisms upon exposure to UV sunlight, this study analyzed (a) the in vitro behavior of fullerene and fullerol against peroxyl radicals (ROO) under UV-A radiation and (b) the effects of these photo-excited NMs on oxidative stress parameters in functional gills extracted from the fish Cyprinus carpio (Cyprinidae). The variables measured were the total antioxidant capacity, lipid peroxidation (TBARS), the activities of the antioxidant enzymes glutathione reductase (GR) and glutamate cysteine ligase (GCL), and the levels of the non-enzymatic antioxidant glutathione (GSH). The obtained results revealed the following: (1) both NMs behaved in vitro as antioxidants against ROO in the dark and as pro-oxidants in presence of UV-A, the latter effect being reversed by the addition of sodium azide, which is a singlet oxygen (¹O₂) quencher; (2) fullerene induced toxicity with or without UV-A incidence, with a significant (p<0.05) increase in lipid peroxidation (with greater damage under illumination), a decrease in GCL activity, and the depletion of GSH stocks (under illumination), all of which were attributed to ¹O₂ generation; and (3) fullerol also decreased GCL activity and GSH formation (p<0.05) but without lipid damage. The overall results show that fullerene can be toxic with or without light incidence, whereas UV radiation seems to play a key role in the environmental toxicity of carbon NMs through ¹O₂ formation.
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology | 2013
Alessandra Martins da Rocha; Josencler L. Ribas Ferreira; Daniela M. Barros; Talita Carneiro Brandão Pereira; Maurício Reis Bogo; Sergio C. Oliveira; Viviany Geraldo; Rodrigo G. Lacerda; Andre S. Ferlauto; Luiz O. Ladeira; M. V. B. Pinheiro; José M. Monserrat
Nanomaterials (NM) industry had grown in the last decade, although there are few studies concerning its potential toxicity effects on aquatic organisms. In this study the freshwater zebrafish (Danio rerio) was exposed to two kinds of carbon NM, single-wall carbon nanotubes (SWCNT) and fullerenol [C60(OH)18-22(OK4)] to analyze oxidative stress responses on fish brain. Adult zebrafish (mean mass: 0.52±0.01g) were submitted to intraperitoneal injections of SWCNT suspension and fullerenol solution (30mg/kg of fish), receiving one or two doses with a time interval of 24h. Results showed that total antioxidant capacity was lowered in brains of fish exposed 24h to fullerenol when compared to those from SWCNT treatment (p<0.05). After 48h, fullerenol induced higher expression of both catalytic and regulatory subunits of enzyme glutamate cysteine ligase when compared to control group (p<0.05), indicating an antioxidant behavior. In vitro assays showed a dual effect of SWCNT, since a pro-oxidant behavior was observed at low concentrations (0.1 and 1.0mg/L) and an antioxidant one at the highest concentration (10.0mg/L). Few biological responses were altered by this NM: decrease in total antioxidant capacity and induction of the expression of the transcription factor Nrf2 when compared to control group.
Journal of Toxicology and Environmental Health | 2012
Roger Stacke Ferreira; José M. Monserrat; Josencler L. Ribas Ferreira; Ana Cristina Kalb; John J. Stegeman; Afonso Celso Dias Bainy; Juliano Zanette
South American cyprinodontiform fish are potential candidates to be used as model biomarker species of exposure in environmental toxicology. The aim of this study was to identify molecular and biochemical biomarkers of pollution using Poecilia vivipara (Poecilidae) and Jenynsia multidentata (Anablepidae). Partial nucleotide sequences for cytochrome P-450 1A (CYP1A), a classical biomarker of exposure to organic contaminants in fish, were identified in P. vivipara and J. multidentata (approximately 650 nucleotides) using degenerated primers and polymerase chain reaction (PCR). These sequences shared approximately 90% identity in the predicted amino acid sequence with the corresponding CYP1A region of Fundulus heteroclitus. Real-time quantitative PCR (RT-qPCR) analysis confirmed that CYP1A transcription was markedly induced in the liver and gills of J. multidentata (approximately185-fold and 20-fold, respectively) and P. vivipara (122-fold and 739-fold, respectively) 24 h after exposure to 1 μM synthetic CYP1A inducer β-naphthoflavone (BNF). At 24 h after injection with 1 μg/g environmental carcinogenic contaminant benzo[a]pyrene (BaP), a decreased total antioxidant capacity against peroxyl radicals was observed both in liver of J. multidentata and gills of P. vivipara. BaP injection in both fish did not produce changes in lipid peroxide (thiobarbituric acid-reactive substances, TBARS) levels, suggesting an absence of an oxidative stress condition. The newly identified CYP1A may thus serve as general biomarker of exposure to organic contaminant in future studies using P. vivipara and J. multidentata. Data also indicate the importance of species-specific differences in biomarker responses in these South American cyprinodontiform fish, suggesting distinct resistance/susceptibility properties to polycyclic aromatic hydrocarbons.
Environmental Toxicology and Chemistry | 2012
Josencler L. Ribas Ferreira; Daniela M. Barros; Laura A. Geracitano; Gilberto Fillmann; Carlos Eduardo Fossa; Eduardo Alves de Almeida; Mariana C. Prado; Bernardo R. A. Neves; M. V. B. Pinheiro; José M. Monserrat
Studies concerning the impact of nanomaterials, especially fullerene (C(60) ), in fresh water environments and their effects on the physiology of aquatic organisms are still scarce and conflicting. We aimed to assess in vitro effects of fullerene in brain and gill homogenates of carp Cyprinus carpio, evaluating redox parameters. A fullerene suspension was prepared by continued stirring under fluorescent light during two months. The suspension concentration was measured by total carbon content and ultraviolet-visible spectroscopy nephelometry. Characterization of C(60) aggregates was performed with an enhanced dark-field microscopy system and transmission electronic microscopy. Organ homogenates were exposed during 1, 2, and 4 h under fluorescent light. Redox parameters evaluated were reduced glutathione and oxidized glutathione, cysteine and cystine, total antioxidant capacity; activity of the antioxidant enzymes glutathione S-transferase and glutathione reductase (GR), and lipid peroxidation (TBARS assay). Fullerene induced a significant increase (p < 0.05) in lipid peroxidation after 2 h in both organs and reduced GR activity after 1 h (gills) and 4 h (brain) and antioxidant capacity after 4 h (brain). Levels of oxidized glutathione increased in the brain at 1 h and decreased at 2 h as well. Given these results, it can be concluded that C(60) can induce redox disruption via thiol/disulfide pathway, leading to oxidative damage (higher TBARS values) and loss of antioxidant competence.
Science of The Total Environment | 2009
Lílian Lund Amado; Márcia Longaray Garcia; Patrícia Ramos; Ricardo F. Freitas; Bruna Zafalon; Josencler L. Ribas Ferreira; João Sarkis Yunes; José M. Monserrat
Comparative Biochemistry and Physiology C-toxicology & Pharmacology | 2012
Carmen L. Azevedo Costa; Isabel Soares Chaves; Juliane Ventura-Lima; Josencler L. Ribas Ferreira; Luis Ferraz; Leandro M. de Carvalho; José M. Monserrat
Pesticide Biochemistry and Physiology | 2016
Francesca M. Mitton; Josencler L. Ribas Ferreira; Mariana Gonzalez; Karina S. B. Miglioranza; José M. Monserrat
Archive | 2013
Juliane Ventura-Lima; Alessandra Martins da Rocha; Marlize Ferreira-Cravo; André da Rosa; Carmen de Azevedo; Isabel Soares Chaves; Josencler L. Ribas Ferreira; Rafaela Letts; Lucas Freitas Cordeiro; Glauce Gouveia; José M. Monserrat