Joseph Sherma

Separation of chloroplast leaf pigments by thin-layer chromatographer on cellulose sheets☆

Abstract Leaf pigments and saponified leaf pigments were separated by one-dimensional, two-dimensional and radial chromatography on Eastman Chromagram Cellulose sheets. Comparisons with Whatman No. 1 paper and conventional cellulose thin layers on glass plates show the Chromagram sheets to be superior in many systems. Comparative results in sandwich tanks and with columns of cellulose are also presented. The major pigments in leaves were found to be chlorophylls a and b , neoxanthin, violaxanthin, lutein and β-carotene.