Joshua Giltinan

Biomedical Applications of Untethered Mobile Milli/Microrobots

Untethered robots miniaturized to the length scale of millimeter and below attract growing attention for the prospect of transforming many aspects of health care and bioengineering. As the robot size goes down to the order of a single cell, previously inaccessible body sites would become available for high-resolution in situ and in vivo manipulations. This unprecedented direct access would enable an extensive range of minimally invasive medical operations. Here, we provide a comprehensive review of the current advances in biomedical untethered mobile milli/microrobots. We put a special emphasis on the potential impacts of biomedical microrobots in the near future. Finally, we discuss the existing challenges and emerging concepts associated with designing such a miniaturized robot for operation inside a biological environment for biomedical applications.

Independent control of multiple magnetic microrobots in three dimensions

A major challenge for untethered microscale mobile robotics is the control of many agents in the same workspace for distributed operation. In this work, we present a new method to independently control multiple sub-mm microrobots in three dimensions (3D) using magnetic gradient pulling as the 3D motion generation method. Motion differentiation is accomplished through the use of geometrically or magnetically distinct microrobots which assume different magnetization directions in a rotating or oscillating magnetic field. This allows for different magnetic forces to be exerted on each, enabling independent motion control and path following of multiple microrobots along arbitrary 3D trajectories. Path following in 3D with less than 310 μ m mean error is shown for a set of two microrobots of size 350 μ m and 1500 μ m, and independent motions are shown with three microrobots. It is also shown that control of more microrobots could be possible using improved magnetic coil hardware. Microrobot diversity is analyzed with regards to the effect on independent control. The proposed addressability method could be used for the 3D control of a team of microrobots inside microfluidic channels or in the human body for localized therapy or diagnostics.

Six-degree-of-freedom magnetic actuation for wireless microrobotics

Existing remotely actuated magnetic microrobots exhibit a maximum of only five-degree-of-freedom (DOF) actuation, as creation of a driving torque about the microrobot magnetization axis is not achievable. This lack of full orientation control limits the effectiveness of existing microrobots for precision tasks of object manipulation and orientation for advanced medical, biological and micromanufacturing applications. This paper presents a magnetic actuation method that allows remotely powered microrobots to achieve full six-DOF actuation by considering the case of a non-uniform magnetization profile within the microrobot body. This non-uniform magnetization allows for additional rigid-body torques to be induced from magnetic forces via a moment arm. A general analytical model presents the working principle for continuous and discrete magnetization profiles, which is applied to permanent or non-permanent (soft) magnet bodies. Several discrete-magnetization designs are also presented which possess reduced coupling between magnetic forces and induced rigid-body torques. Design guidelines are introduced which can be followed to ensure that a magnetic microrobot design is capable of six-DOF actuation. A simple permanent-magnet prototype is fabricated and used to quantitatively demonstrate the accuracy of the analytical model in a constrained-DOF environment and qualitatively for free motion in a viscous liquid three-dimensional environment. Results show that desired forces and torques can be created with high precision and limited parasitic actuation, allowing for full six-DOF actuation using limited feedback control.

Programmable assembly of heterogeneous microparts by an untethered mobile capillary microgripper

At the sub-millimeter scale, capillary forces enable robust and reversible adhesion between biological organisms and varied substrates. Current human-engineered mobile untethered micromanipulation systems rely on forces which scale poorly or utilize gripper-part designs that promote manipulation. Capillary forces, alternatively, are dependent upon the surface chemistry (which is scale independent) and contact perimeter, which conforms to the part surface. We report a mobile capillary microgripper that is able to pick and place parts of various materials and geometries, and is thus ideal for microassembly tasks that cannot be accomplished by large tethered manipulators. We achieve the programmable assembly of sub-millimeter parts in an enclosed three-dimensional aqueous environment by creating a capillary bridge between the targeted part and a synthetic, untethered, mobile body. The parts include both hydrophilic and hydrophobic components: hydrogel, kapton, human hair, and biological tissue. The 200 μm untethered system can be controlled with five-degrees-of-freedom and advances progress towards autonomous desktop manufacturing for tissue engineering, complex micromachines, microfluidic devices, and meta-materials.

Six-Degrees-of-Freedom Remote Actuation of Magnetic Microrobots.

Existing remotely-actuated microrobots powered by magnetic coils far from the workspace exhibit a maximum of only five-degrees-of-freedom (DOF) actuation, as creation of a driving torque about the magnetization axis is not achievable. This lack of orientation control limits the effectiveness of existing microrobots for precision tasks of object manipulation and orientation for advanced medical, biological and micro-manufacturing applications. This paper presents a magnetic actuation method that allows these robots to achieve full six-DOF actuation by allowing for a non-uniform magnetization profile within the microrobot body. This non-uniform magnetization results in additional rigid-body torques to be induced from magnetic forces via a moment arm. A general analytical model presents the working principle for continuous and discrete magnetization profiles. Using this model, microrobot design guidelines are introduced which guarantee six-DOF actuation capability. Several discrete magnetization designs which possess reduced coupling between magnetic forces and induced rigid-body torques are also presented. A simple permanent-magnet decoupled prototype is fabricated and used to quantitatively demonstrate the accuracy of the analytical model in a constrained-DOF environment and qualitatively for free motion in a viscous liquid three-dimensional environment. Results show that desired forces and torques can be created with high precision and limited parasitic actuation, allowing for full sixDOF actuation using limited feedback control.

Three-dimensional robotic manipulation and transport of micro-scale objects by a magnetically driven capillary micro-gripper

One major challenge for untethered micro-scale mobile robotics is the manipulation of external objects in the robots three-dimensional (3D) work environment. Here, we present a method to use the capillary force at a solid-liquid-gas interface to reversibly attach objects to a mobile magnetic microrobot. This is accomplished by the addition of a cavity in the hydrophobic microrobot, in which an air bubble is captured when the microrobot is placed in a water environment. The extension of the air bubble from the cavity is adjusted dynamically by controlling the pressure of the workspace environment. A peak switching ratio between the maximum and minimum gripping forces of 14:1 is shown for controlled attachment/detachment experiments, which allows for reliable pick-and-place operation. This work introduces an analytical capillary adhesion model and demonstrates control of the bubble size for pick-and-place gripping. A proof-of-concept demonstration of 3D manipulation in a fluidic environment shows the potential of capillary gripping for future use in confined environments such as inside microfluidic devices for transportation or assembly of hydrophobic objects.

Three dimensional independent control of multiple magnetic microrobots

A major challenge for untethered micro-scale mobile robotics is the control of many agents in the same workspace for distributed operation. In this work, we present a new method to independently control multiple sub-mm microrobots in three dimensions (3D) using magnetic gradient based direct pulling as the 3D motion generation method. This is accomplished through the use of geometrically or magnetically distinct microrobots which assume different magnetization directions in a rotating magnetic field. Such diversity in design allows for different magnetic forces to be exerted on each, enabling path following with less than 370μm mean path deviation for a set of two microrobots of size 350μm and 1500μm. This addressability method could be used for the 3D control of a team of microrobots inside microfluidic channels or in the human body for localized therapy or diagnostics.

Soft erythrocyte-based bacterial microswimmers for cargo delivery

Erythrocyte-based microswimmers offer superior efficiency, stability, and deformability for active and guided cargo delivery. Bacteria-propelled biohybrid microswimmers have recently shown to be able to actively transport and deliver cargos encapsulated into their synthetic constructs to specific regions locally. However, usage of synthetic materials as cargo carriers can result in inferior performance in load-carrying efficiency, biocompatibility, and biodegradability, impeding clinical translation of biohybrid microswimmers. Here, we report construction and external guidance of bacteria-driven microswimmers using red blood cells (RBCs; erythrocytes) as autologous cargo carriers for active and guided drug delivery. Multifunctional biohybrid microswimmers were fabricated by attachment of RBCs [loaded with anticancer doxorubicin drug molecules and superparamagnetic iron oxide nanoparticles (SPIONs)] to bioengineered motile bacteria, Escherichia coli MG1655, via biotin-avidin-biotin binding complex. Autonomous and on-board propulsion of biohybrid microswimmers was provided by bacteria, and their external magnetic guidance was enabled by SPIONs loaded into the RBCs. Furthermore, bacteria-driven RBC microswimmers displayed preserved deformability and attachment stability even after squeezing in microchannels smaller than their sizes, as in the case of bare RBCs. In addition, an on-demand light-activated hyperthermia termination switch was engineered for RBC microswimmers to control bacteria population after operations. RBCs, as biological and autologous cargo carriers in the biohybrid microswimmers, offer notable advantages in stability, deformability, biocompatibility, and biodegradability over synthetic cargo-carrier materials. The biohybrid microswimmer design presented here transforms RBCs from passive cargo carriers into active and guidable cargo carriers toward targeted drug and other cargo delivery applications in medicine.

Dynamic and programmable self-assembly of micro-rafts at the air-water interface

We built an out-of-equilibrium material system by designing competing interactions that are both dissipative and programmable. Dynamic self-assembled material systems constantly consume energy to maintain their spatiotemporal structures and functions. Programmable self-assembly translates information from individual parts to the collective whole. Combining dynamic and programmable self-assembly in a single platform opens up the possibilities to investigate both types of self-assembly simultaneously and to explore their synergy. This task is challenging because of the difficulty in finding suitable interactions that are both dissipative and programmable. We present a dynamic and programmable self-assembling material system consisting of spinning at the air-water interface circular magnetic micro-rafts of radius 50 μm and with cosinusoidal edge-height profiles. The cosinusoidal edge-height profiles not only create a net dissipative capillary repulsion that is sustained by continuous torque input but also enable directional assembly of micro-rafts. We uncover the layered arrangement of micro-rafts in the patterns formed by dynamic self-assembly and offer mechanistic insights through a physical model and geometric analysis. Furthermore, we demonstrate programmable self-assembly and show that a 4-fold rotational symmetry encoded in individual micro-rafts translates into 90° bending angles and square-based tiling in the assembled structures of micro-rafts. We anticipate that our dynamic and programmable material system will serve as a model system for studying nonequilibrium dynamics and statistical mechanics in the future.

Addressing of Micro-robot Teams and Non-contact Micro-manipulation

This manuscript presents two methods for the addressable control of multiple magnetic microrobots. Such methods could be valued for microrobot applications requiring high speed parallel operation. The first uses multiple magnetic materials to enable selective magnetic disabling while the second allows for independent magnetic forces to be applied to a set of magnetic micro-robots moving in three dimensions. As an application of untethered magnetic microrobots, we also present a non-contact manipulation method for micron scale objects using a locally induced rotational fluid flow field. The micro-manipulator is rotated by an external magnetic field in a viscous fluid to generate a rotational flow field, which moves the objects in the flow region by fluidic drag. Due to its untethered and non-contact operation, this micro-manipulation method could be used to quickly move fragile micro-objects in inaccessible or enclosed spaces such as in lab-on-a-chip devices. In addition to introducing the operation and capability of these fabrication and control methods, we discuss the implications of scaling these systems to smaller scales for comparison with other microrobotics actuation and control techniques.