Jouni Kekoni

Human somatosensory evoked potentials to mechanical pulses and vibration: contributions of SI and SII somatosensory cortices to P50 and P100 components ☆

Somatosensory evoked potentials (SEPs) were measured to short tactile pulses and vibratory stimuli applied to the fingertip to determine the characteristics and scalp topography of different early and late SEP components to these types of stimulus. The measurements were obtained from 3 homologous contra- and ipsilateral locations and from the vertex. In 2 subjects the SEPs were measured from 23 recording locations. The subjects were reading during the experiments. The first distinct contralateral response was an anteriorly negative and centrally as well as posteriorly positive peak at about 50 msec latency (P50). Largest P50 responses with shortest peak latencies were measured to single tactile pulses. We suggest that P50 is probably generated in the contralateral SI cortex. The P50 was followed by a distinct negative deflection (N70) in the middle and posterior recording locations on the contralateral hemisphere, which reversed its polarity in the frontal records. This peak was also seen ipsilaterally. At about 100 msec latency a distinct bilateral positive P100 peak was obtained. This peak was most prominent to vibratory stimuli, and especially to high frequency vibration. Comparisons with recent intracortical SEP studies in primates and MEG studies in humans suggest that P100 might be best accounted for by bilateral generators in SII cortices. The early components were followed by a negative N140 wave and by a slow, positive wave with a maximum at about 300 msec. Both waves had an asymmetrical distribution. The N140 wave occurred bilaterally, but was largest contralaterally, and often had two peaks at posterior recording locations. The slow positivity was largest at the vertex and at mid-posterior recording sites.

Auditory and somatosensory event-related brain potentials in early blind humans.

Previous event-related potential (ERP) studies have suggested a possible participation of the visual cortex of the blind in auditory processing. In the present study, somatosensory and auditory ERPs of blind and sighted subjects were recorded when subjects were instructed to attend to stimuli of one modality and to ignore those of the other. Both modalities were stimulated with frequent (“standard”) and infrequent (“deviant”) stimuli, which differed from one another in their spatial locus of origin. In the sighted, deviant stimuli of the attended modality elicited N2 type of deflections (auditory N2b and somatosensory N250) over the lateral scalp areas. In contrast, in the blind, these ERP components were centroposteriorly distributed, suggesting an involvement of posterior brain areas in auditory and somatosensory stimulus discrimination. In addition, the mismatch negativity, elicited by deviant auditory stimuli even when the somatosensory stimuli were attended, was larger in the blind than in the sighted. This appears to indicate enhanced automatic processing of auditory stimulus changes in the blind. Thus, the present data suggest several compensatory changes in both auditory and somatosensory modalities after the onset of early visual deprivation.

Rate effect and mismatch responses in the somatosensory system: ERP-recordings in humans

In the first experiment, somatosensory event-related potentials (SERPs) were recorded to tactile pulses and vibration bursts applied to the left middle finger in trains of 4-8 stimuli with 1 s intervals. In addition to the negative N140 deflection, also the positive P50, P100 and P300 waves attenuated in amplitude with stimulus repetition. These decreases were immediate, there being no marked further amplitude attenuation after the second stimulus. The locus of this rate effect appears not to be the primary SI or SII, but rather prefrontal cortices or some deeper structures. In the second experiment, vibratory stimuli of different frequencies or at different skin sites were presented using the oddball paradigm. When the deviant stimulus was a high-frequency vibration burst, it elicited a distinct N250 deflection, probably analogous to the auditory N2b. When the deviation was a change in the stimulation site, no N250 deflection but instead an extra negativity between 100-200 ms latency, probably analogous to the auditory mismatch negativity, was observed.

Mechanical sensibility of the sole of the foot determined with vibratory stimuli of varying frequency

SummaryThe mechanoreceptive properties of the sole of the foot were determined by measuring the detection thresholds to vibratory stimuli of 20, 80, and 240 Hz frequency and 300 ms duration. The thresholds were measured at six different sites on the left sole and at toes 1 and 3 with probes of 2 and 8 mm diameter connected to the moving coil of an electromechanical vibrator. The subject sat in an armchair during the experiments, with the left leg supported horizontally by a vacuum cast positioned on a table. Six subjects participated in the experiments. A simple method of limits was used to make the measurements. Lower average thresholds were obtained with higher vibration frequencies, the average thresholds varying between 40–90 μm at 20 Hz and well below 10 μm at 240 Hz. The major decrease in thresholds occurred between 20 and 80 Hz. Interindividual variability in thresholds was large, but the threshold curves obtained from different subjects and from different stimulation points were of the same general shape. The highest thresholds were measured from the toes, but this regional difference in sensibility was obtained only at the higher vibration frequencies. Comparison of the threshold values at the sole with those found with similar stimuli at the thenar eminence and middle fingertip indicates that the mechanoreceptor mechanisms transmitting information about low-frequency vibration in the sole are similar to those in the palmar skin of the hand.

A spatial oculomotor memory-task performance produces a task-related slow shift in human electroencephalography

Electroencephalographic (EEG) deflections in humans related to the performance of memory-guided saccades were studied in this work. The EEG deflections were recorded during 2 spatial oculomotor delayed response tasks in which the subject was instructed to make a saccade either to the right or to the left depending on the spatial location of the cue which had been shown in the beginning of the delay period. The EEG deflections were compared to those recorded during a control task in which the subject also made a saccade to the right or to the left after a delay but the requirement to keep spatial information actively in mind was minimized. A slow delay-related shift was recorded during all task conditions. The slow shift was positive in the most frontal and negative in the more posterior recording sites. The negative slow shift in the more posterior recording sites was larger in the memory tasks than in the control task. Since the memory and the control tasks differed mainly in their requirement to hold spatial information in mind it is suggested that the difference in the magnitude of slow shifts between the memory and the control tasks reflects neural activity related to spatial working memory. But although the oculomotor responses in all tasks were similar, the preparatory activities for the impending eye movements may not have been similar and in addition to working memory may have contributed to the observed differences in the slow shifts.

Visuospatial mnemonic load modulates event-related slow potentials.

ELECTROENCEPHALOGRAPHIC slow wave potentials were recorded during the performance of visuospatial working memory tasks. The aim was to study the effects of varying mnemonic loads on slow potentials, and to dissociate the contribution of mnemonic and motor components. Subjects were tested with three spatial delayed matching-to-sample tasks in which the mnemonic load varied while the preparatory motor demands remained constant. The delay-related slow potential was more negative during the tasks in which the subjects had to memorize the locations of six or four stimuli than when only one location had to be memorized. Significant differences between the slow potentials in the tasks with different mnemonic loads were recorded at frontal and temporal recording sites. Since the preparatory motor requirements were similar in all tasks, the modulation of slow potentials reflects working memory processing rather than motor preparatory activity.

Effect of unilateral sensory impairment of the sole of the foot on postural control in man: Implications for the role of mechanoreception in postural control

Abstract Body sway was measured in normal subjects and in patients with impaired mechanical sensitivity in limited areas of one or the other sole due to microvascular free flap reconstruction for a traumatic defect. This approach was used for evaluating the role of mechanoreception of the soles in postural control, and for determining the extent to which the sensitivity decrement affected the ability of these patients to control their posture. Mechanical sensibility was determined with vibratory stimuli of different frequencies which preferentially activate receptors of different types in the skin. Body sway was measured with a force platform technique with the eyes open and also closed. In both controls and patients the total extent of sway was larger when the eyes were kept closed. With the eyes open, the patients showed a normal extent of sway, but with the eyes closed the sway increased more than in the controls. The amount of sway increased both with the severity of the sensory impairment, and with the size of the affected skin region.

Fast decrement with stimulus repetition in ERPs generated by neuronal systems involving somatosensory SI and SII cortices: Electric and magnetic evoked response recordings in humans

The effect of stimulus repetition (short trains of stimuli with 1-s inter-stimulus intervals and 15-s inter-train intervals) on both electric and magnetic evoked responses were studied in four subjects. In addition to the later N140 and P300 deflections in electric potentials, a distinct and immediate amplitude decrement was obtained also for the earlier P50 and P100 deflections. The magnetic evoked responses also demonstrated the amplitude decrement for 50 ms (M50) and 100 ms (M100) latency deflections. The time-course and degree of amplitude decrement of the M100 magnetic response corresponded especially well to those of P100 electric deflections. The results thus show the rate effect on electric and magnetic responses at 50 and 100 ms latencies, and further suggest that the electric and magnetic responses, reflecting the activation of somatosensory SI and SII cortical areas at these latencies, respectively, are generated by related neuronal mechanisms.

Spatial Features of Vibrotactile Masking Effects on Airpuff-Elicited Sensations in the Human Hand

It was recently shown that the cutaneous sensitivity to airpuffs is decreased by a low-frequency vibrotactile masker in the hairy skin, and by a low-frequency but especially by a high-frequency masker in the glabrous skin. In the current study, the spatial features of this masking effect were determined in four healthy human subjects, using a reaction time paradigm. The masking effect decreased monotonically with increasing interstimulus distance, and identically in longitudinal and transverse (i.e., lateral) directions in the palm or dorsal surface of the hand. The masking effect was stronger in the glabrous than in the hairy skin, especially in the fingers. In the glabrous skin, the spread of masking effect produced by a high-frequency masker was more extensive than that produced by a low-frequency masker. The mechanical spread of high-frequency vibration was less extensive than that of low-frequency vibration in the skin. In the glabrous skin, a masker applied to the tip of the finger produced a stronger masking effect on sensations in the base of the finger than when the masker was located at the base and the test stimulus was located at the tip. It is concluded that mechanical spread of vibration in the skin is of minor importance in explaining the masking effects. Different peripheral neural mechanisms underlie the airpuff-elicited sensations in the hairy and glabrous skin. The afferent inhibitory mechanisms are stronger for signals coming from the glabrous skin of the fingers than for signals coming from the hairy skin. Furthermore, the peripheral innervation density and size of the cortical representational areas may be of importance in determining the magnitude of the masking effect.

Vibrotactile Masking Effects on Airpuff-Elicited Sensations Vary with Skin Region in the Human Hand

Inhibitory interactions between two tactile signals take place predominantly within mechanoreceptive submodality channels. This finding was utilized in the present study to determine the mechanoreceptive channels contributing to tactile sensations elicited by brief airpuff stimuli applied to the hairy and glabrous skin of the human hand. A reaction time paradigm was used to estimate the sensitivity of four subjects to airpuffs without and during continuous vibration (masker) of low (30 Hz) or high (240 Hz) frequency. The sensitivity to airpuffs (test stimuli) was decreased by a low-frequency masker in the hairy skin and by low- and especially by high-frequency maskers in the glabrous skin. The masking effect was enhanced in both skin areas by increasing the intensity of the masker and by decreasing the intensity of the test stimulus. The results suggest that the mechanisms underlying airpuff-elicited sensations consist of the low-frequency channel in the hairy skin, and of both the low- and high-frequency channels in the glabrous skin.