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Dive into the research topics where Juçara R. Franca is active.

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Featured researches published by Juçara R. Franca.


Investigative Ophthalmology & Visual Science | 2013

Antiglaucomatous effects of the activation of intrinsic Angiotensin-converting enzyme 2.

Giselle Foureaux; José Carlos Nogueira; Bárbara S. Nogueira; Gustavo de Oliveira Fulgêncio; Gustavo B. Menezes; Simone Odília Antunes Fernandes; Valbert Nascimento Cardoso; Renata S. Fernandes; Gabriel P. Oliveira; Juçara R. Franca; André Augusto Gomes Faraco; Mohan K. Raizada; Anderson J. Ferreira

PURPOSE To evaluate the effects of the activation of endogenous angiotensin-converting enzyme 2 (ACE2) using the compound diminazene aceturate (DIZE) in an experimental model of glaucoma in Wistar rats. METHODS DIZE (1 mg/kg) was administered daily, either systemically or topically, and the IOP was measured weekly. To examine the role of the Mas receptor in the effects of DIZE, the Ang-(1-7) antagonist A-779 was co-administered. Drainage of the aqueous humor was evaluated by using scintigraphy. The analysis of ACE2 expression by immunohistochemistry and the counting of retinal ganglion cells (RGCs) were performed in histologic sections. Additionally, the nerve fiber structure was evaluated by transmission electron microscopy. RESULTS The systemic administration and topical administration (in the form of eye drops) of DIZE increased the ACE2 expression in the eyes and significantly decreased the IOP of glaucomatous rats without changing the blood pressure. Importantly, this IOP-lowering action of DIZE was similar to the effects of dorzolamide. The antiglaucomatous effects of DIZE were blocked by A-779. Histologic analysis revealed that the reduction in the number of RGCs and the increase in the expression of caspase-3 in the RGC layer in glaucomatous animals were prevented by DIZE. This compound also prevented alterations in the cytoplasm of axons in glaucomatous rats. In addition to these neuroprotective effects, DIZE facilitated the drainage of the aqueous humor. CONCLUSIONS Our results evidence the pathophysiologic relevance of the ocular ACE2/Ang-(1-7)/Mas axis of the renin-angiotensin system and, importantly, indicate that the activation of intrinsic ACE2 is a potential therapeutic strategy to treat glaucoma.


International Journal of Nanomedicine | 2014

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Tatiana G. Ribeiro; Miguel A. Chávez-Fumagalli; Diogo G. Valadares; Juçara R. Franca; Lívia Bomfim Rodrigues; Mariana C. Duarte; Paula S. Lage; Pedro Henrique Rocha de Andrade; Daniela P. Lage; Leonardo V. Arruda; Daniel R. Abánades; Lourena E. Costa; Vívian T. Martins; Carlos Ap Tavares; Rachel Oliveira Castilho; Eduardo Af Coelho; André Ag Faraco

The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) – were used: Cs as a positively charged polymer and ChS as a negatively charged polymer. The chitosan (NQ) nanoparticles, chitosan-chondroitin sulfate (NQC) nanoparticles, and chitosan-chondroitin sulfate-amphotericin B (NQC-AmpB) nanoparticles presented a mean particle size of 79, 104, and 136 nm, respectively; and a polydispersity index of 0.2. The measured zeta potential of the nanoparticles indicated a positive charge in their surface, while scanning and transmission electron microscopy revealed spherical nanoparticles with a smooth surface. Attenuated total reflectance-Fourier transform infrared spectroscopy analysis showed an electrostatic interaction between the polymers, whereas the release profile of AmpB from the NQC-AmpB nanoparticles showed a controlled release. In addition, the Cs; ChS; and NQ, NQC, and NQC-AmpB nanoparticles proved to be effective against promastigotes of Leishmania amazonensis and Leishmania chagasi, with a synergistic effect observed between Cs and ChS. Moreover, the applied NQ, NQC, and NQC-AmpB compounds demonstrated low toxicity in murine macrophages, as well as null hemolytic activity in type O+ human red blood cells. Pure AmpB demonstrated high toxicity in the macrophages. The results show that cells infected with L. amazonensis and later treated with Cs, ChS, NQ, NQC, NQC-AmpB nanoparticles, or pure AmpB presented with a significant reduction in parasite number in the order of 24%, 31%, 55%, 66%, 90%, and 89%, respectively. The data presented indicate that the engineered NQC-AmpB nanoparticles could potentially be used as an alternative therapy to treat leishmaniasis, mainly due its low toxicity to mammals’ cells.


PLOS ONE | 2014

Bimatoprost-Loaded Ocular Inserts as Sustained Release Drug Delivery Systems for Glaucoma Treatment: In Vitro and In Vivo Evaluation

Juçara R. Franca; Giselle Foureaux; Leonardo Lima Fuscaldi; Tatiana G. Ribeiro; Lívia Bomfim Rodrigues; Renata Bravo; Rachel Oliveira Castilho; Maria Irene Yoshida; Valbert Nascimento Cardoso; Simone Odília Antunes Fernandes; Sebastião Cronemberger; Anderson J. Ferreira; André Augusto Gomes Faraco

The purpose of the present study was to develop and assess a novel sustained-release drug delivery system of Bimatoprost (BIM). Chitosan polymeric inserts were prepared using the solvent casting method and characterized by swelling studies, infrared spectroscopy, differential scanning calorimetry, drug content, scanning electron microscopy and in vitro drug release. Biodistribution of 99mTc-BIM eye drops and 99mTc-BIM-loaded inserts, after ocular administration in Wistar rats, was accessed by ex vivo radiation counting. The inserts were evaluated for their therapeutic efficacy in glaucomatous Wistar rats. Glaucoma was induced by weekly intracameral injection of hyaluronic acid. BIM-loaded inserts (equivalent to 9.0 µg BIM) were administered once into conjunctival sac, after ocular hypertension confirmation. BIM eye drop was topically instilled in a second group of glaucomatous rats for 15 days days, while placebo inserts were administered once in a third group. An untreated glaucomatous group was used as control. Intraocular pressure (IOP) was monitored for four consecutive weeks after treatment began. At the end of the experiment, retinal ganglion cells and optic nerve head cupping were evaluated in the histological eye sections. Characterization results revealed that the drug physically interacted, but did not chemically react with the polymeric matrix. Inserts sustainedly released BIM in vitro during 8 hours. Biodistribution studies showed that the amount of 99mTc-BIM that remained in the eye was significantly lower after eye drop instillation than after chitosan insert implantation. BIM-loaded inserts lowered IOP for 4 weeks, after one application, while IOP values remained significantly high for the placebo and untreated groups. Eye drops were only effective during the daily treatment period. IOP results were reflected in RGC counting and optic nerve head cupping damage. BIM-loaded inserts provided sustained release of BIM and seem to be a promising system for glaucoma management.


Experimental Parasitology | 2014

Antileishmanial activity and cytotoxicity of Brazilian plants

Tatiana G. Ribeiro; Miguel A. Chávez-Fumagalli; Diogo G. Valadares; Juçara R. Franca; Paula S. Lage; Mariana C. Duarte; Pedro Henrique Rocha de Andrade; Vívian T. Martins; Lourena E. Costa; Ana Lúcia A. Arruda; André Augusto Gomes Faraco; Eduardo Antonio Ferraz Coelho; Rachel Oliveira Castilho

Leishmaniasis is a major public health problem, and the alarming spread of parasite resistance has increased the importance of discovering new therapeutic products. The present study aimed to investigate the in vitro leishmanicidal activity from 16 different Brazilian medicinal plants. Stationary-phase promastigotes of Leishmania amazonensis and murine macrophages were exposed to 44 plant extracts or fractions for 48 h at 37°C, in order to evaluate their antileishmanial activity and cytotoxicity, respectively. The most potent extracts against L. amazonensis were the hexanic extract of Dipteryx alata (IC50 of 0.08 μg/mL), the hexanic extract of Syzygium cumini (IC50 of 31.64 μg/mL), the ethanolic and hexanic extracts of leaves of Hymenaea courbaril (IC50 of 44.10 μg/mL and 35.84 μg/mL, respectively), the ethanolic extract of H. stignocarpa (IC50 of 4.69 μg/mL), the ethanolic extract of Jacaranda caroba (IC50 of 13.22 μg/mL), and the ethanolic extract of J. cuspidifolia leaves (IC50 of 10.96 μg/mL). Extracts of D. alata and J. cuspidifolia presented higher selectivity index, with high leishmanicidal activity and low cytotoxicity in the mammalian cells. The capacity in treated infected macrophages using the extracts and/or fractions of D. alata and J. cuspidifolia was also analyzed, and reductions of 95.80%, 98.31%, and 97.16%, respectively, in the parasite burden, were observed. No nitric oxide (NO) production could be observed in the treated macrophages, after stimulation with the extracts and/or fractions of D. alata and J. cuspidifolia, suggesting that the biological activity could be due to mechanisms other than macrophage activation mediated by NO production. Based on phytochemistry studies, the classes of compounds that could contribute to the observed activities are also discussed. In conclusion, the data presented in this study indicated that traditional medicinal plant extracts present effective antileishmanial activity. Future studies could focus on the identification and purification of the antileishmanial compounds within these plants for analysis of their in vivo antileishmanial activity.


BMC Complementary and Alternative Medicine | 2014

Propolis--based chitosan varnish: drug delivery, controlled release and antimicrobial activity against oral pathogen bacteria.

Juçara R. Franca; Mariana Passos De Luca; Tatiana G. Ribeiro; Rachel Oliveira Castilho; Allyson Nogueira Moreira; Vagner Rodrigues Santos; André Ag Faraco

BackgroundDental caries is the most prevalent oral disease in several Asian and Latin American countries. It is an infectious disease and different types of bacteria are involved in the process. Synthetic antimicrobials are used against this disease; however, many of these substances cause unwarranted undesirable effects like vomiting, diarrhea and tooth staining. Propolis, a resinous substance collected by honeybees, has been used to control the oral microbiota. So, the objective of this study was to develop and characterize sustained-release propolis-based chitosan varnish useful on dental cariogenic biofilm prevention, besides the in vitro antimicrobial activity.MethodsThree formulations of propolis - based chitosan varnish (PCV) containing different concentrations (5%, 10% and 15%) were produced by dissolution of propolis with chitosan on hydro-alcoholic vehicle. Bovine teeth were used for testing adhesion of coatings and to observe the controlled release of propolis associated with varnish. It was characterized by infrared spectroscopy, scanning electron microscopy, casting time, diffusion test in vitro antimicrobial activity and controlled release. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested for the main microorganisms involved in the cariogenic biofilm through the microdilution test in 96-well plates.ResultsThe formulations presented a tooth surface adherence and were able to form films very fast on bovine tooth surface. Also, propolis-based chitosan varnishes have shown antimicrobial activity similar to or better than chlorhexidine varnish against all oral pathogen bacteria. All microorganisms were sensitive to propolis varnish and chitosan. MIC and MBC for microorganisms of cariogenic biofilme showed better results than chlorhexidine. Propolis active components were released for more than one week.ConclusionAll developed formulations turn them, 5%, 10% and 15% propolis content varnish, into products suitable for clinical application on dental caries prevention field, deserving clinical studies to confirm its in vivo activity.


BioMed Research International | 2014

Propolis varnish: antimicrobial properties against cariogenic bacteria, cytotoxicity, and sustained-release profile.

Mariana Passos De Luca; Juçara R. Franca; Filipe Augusto F. F. Macedo; Liliana Grenho; Maria E. Cortés; André Augusto Gomes Faraco; Allyson Nogueira Moreira; Vagner Rodrigues Santos

Varnishes are preparations that differ in the polymeric matrix and therapeutical agents. In dentistry they are used to prevent caries. In this study we developed a propolis varnish, considering propolis properties against cariogenic bacteria. To a chitosan polymeric base (CHV) was added ethanolic propolis extract in different concentrations: PV1 (5%), PV2 (10%), and PV3 (15%). Antimicrobial activity was carried out against Streptococcus mutans (SM), Streptococcus sanguinis (SG), Streptococcus salivarius (SS), and Lactobacillus casei (LC) through agar diffusion method. The three propolis concentrations incorporated were effective in inhibiting the growth of all microorganisms, but without significant difference between the zones of inhibition observed. Cytotoxicity assay was done by MTT method. Data were analyzed by one-way ANOVA and Bonferroni test. None of the varnishes were cytotoxic, keeping 80% of viable cells, while CHV allowed cellular proliferation (120%). Sustained-release test was carried out by applying 40 μL of each varnish in the buccal surface of bovine teeth and kept in an ethanol/water solution removed in regular times. According to the “independent model approach,” the release profiles were distinct from each varnish and the most prolonged was PV3 (8 weeks). Varnish formulations had satisfactory antimicrobial activity against cariogenic bacteria and have a low cytotoxicity (<50%).


Journal of Ethnopharmacology | 2015

Antileishmanial activity of standardized fractions of Stryphnodendron obovatum (Barbatimão) extract and constituent compounds.

Tatiana G. Ribeiro; André Márcio do Nascimento; Bárbara O. Henriques; Miguel A. Chávez-Fumagalli; Juçara R. Franca; Mariana C. Duarte; Paula S. Lage; Pedro Henrique Rocha de Andrade; Daniela P. Lage; Lívia Bomfim Rodrigues; Lourena E. Costa; Vívian T. Martins; André Augusto Gomes Faraco; Eduardo Antonio Ferraz Coelho; Rachel Oliveira Castilho

ETHNOPHARMACOLOGICAL RELEVANCE Stryphnodendron obovatum Benth. is a Brazilian tree used to treat skin ulceration, promote wound healing, and inhibit the growth of protozoa, including Trypanosoma and Leishmania species. Bioguided fractionation of the ethanol extract of S. obovatum stem bark was performed, and antileishmanial and antioxidant activities of the standardized fractions were analyzed. MATERIALS AND METHODS Stationary-phase Leishmania amazonensis promastigotes, murine macrophages, and human red blood cells (RBCs) were exposed to plant extract, standardized fractions or isolated compounds for 48 h at 37 °C to evaluate their antiparasitic activity and cytotoxicity. The 2,2-diphenyl-1-picryl-hidrazyl assay was used to evaluate antioxidant activity. RESULTS The S. obovatum extract and fractions showed antileishmanial and antioxidant activity; however, the organic fraction (OF) showed the best efficacy. We identified gallic acid, gallocatechin, epigallocatechin, catechin, and epigallocatechin gallate in the OF fraction. These compounds effectively inhibited L. amazonensis activity, with gallic acid, gallocatechin, and epigallocatechin gallate showing the highest selectivity. Furthermore, the evaluated compounds had no significant effect on murine macrophages and human RBCs. CONCLUSIONS The compounds present in the S. obovatum plant bark ethanol extract may provide an alternative therapeutic approach for L. amazonensis treatment.


PLOS ONE | 2015

Ocular Inserts for Sustained Release of the Angiotensin-Converting Enzyme 2 Activator, Diminazene Aceturate, to Treat Glaucoma in Rats

Giselle Foureaux; Juçara R. Franca; José Carlos Nogueira; Gustavo de Oliveira Fulgêncio; Tatiana G. Ribeiro; Rachel Oliveira Castilho; Maria Irene Yoshida; Leonardo Lima Fuscaldi; Simone Odília Antunes Fernandes; Valbert Nascimento Cardoso; Sebastião Cronemberger; André Augusto Gomes Faraco; Anderson J. Ferreira

The aim of this study was to develop and evaluate the effects of chitosan inserts for sustained release of the angiotensin-converting enzyme 2 (ACE2) activator, diminazene aceturate (DIZE), in experimental glaucoma. Monolayer DIZE loaded inserts (D+I) were prepared and characterized through swelling, attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and in vitro drug release. Functionally, the effects of D+I were tested in glaucomatous rats. Glaucoma was induced by weekly injections of hyaluronic acid (HA) into the anterior chamber and intraocular pressure (IOP) measurements were performed. Retinal ganglion cells (RGC) and optic nerve head cupping were evaluated in histological sections. Biodistribution of the drug was accessed by scintigraphic images and ex vivo radiation counting. We found that DIZE increased the swelling index of the inserts. Also, it was molecularly dispersed and interspersed in the polymeric matrix as a freebase. DIZE did not lose its chemical integrity and activity when loaded in the inserts. The functional evaluation demonstrated that D+I decreased the IOP and maintained the IOP lowered for up to one month (last week: 11.0±0.7 mmHg). This effect of D+I prevented the loss of RGC and degeneration of the optic nerve. No toxic effects in the eyes related to application of the inserts were observed. Moreover, biodistribution studies showed that D+I prolonged the retention of DIZE in the corneal site. We concluded that D+I provided sustained DIZE delivery in vivo, thereby evidencing the potential application of polymeric-based DIZE inserts for glaucoma management.


Analytical Letters | 2015

Development and Validation of a High Performance Liquid Chromatographic Method for Determination of Bimatoprost in Chitosan-Based Ocular Inserts

Juçara R. Franca; Luciana D. Batista; Tatiana G. Ribeiro; Christian Fernandes; Rachel Oliveira Castilho; André Augusto Gomes Faraco

Glaucoma is one of the most prominent causes of blindness around the world. Conventional eye-drops, currently used in the treatment of the disease, must be taken every day and can cause severe systemic side effects. In order to address these problems, a solvent casting method was used to produce chitosan-based polymeric ocular inserts for controlled release of bimatoprost, a prostaglandin analog highly powerful in reducing intraocular pressure. The development and validation of quality control methods are an important concern in the field of pharmaceutical technology when dealing with new drug delivery systems. In this context, a rapid method using reverse phase high-performance liquid chromatography with ultraviolet detection was developed and validated for the determination of the pharmaceutical into the chitosan-based polymeric matrix. The method was linear (determination coefficient higher than 0.99) over the range of 3.00–15.00 µg/mL, precise (relative standard deviation lower than 5%), accurate (recovery of 104.09%), selective, and had limits of quantification and detection of 0.90 and 0.60 µg/mL, respectively. The validated method was successfully employed for assay and performance measurements of bimatoprost in the developed formulation.


Advances in Bioscience and Biotechnology | 2013

Development and characterization of polymeric nanoparticles as Barbatimão ( Stryphnodendron obovatum ) standardized fraction carrier

Daniel Ferreira Costa; Juçara R. Franca; Tatiana G. Ribeiro; Maria Auxiliadora Coelho Kaplan; André Augusto Gomes Faraco; Rachel Oliveira Castilho

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André Augusto Gomes Faraco

Universidade Federal de Minas Gerais

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Rachel Oliveira Castilho

Universidade Federal de Minas Gerais

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Tatiana G. Ribeiro

Universidade Federal de Minas Gerais

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Allyson Nogueira Moreira

Universidade Federal de Minas Gerais

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Anderson J. Ferreira

Universidade Federal de Minas Gerais

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Giselle Foureaux

Universidade Federal de Minas Gerais

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Vagner Rodrigues Santos

Universidade Federal de Minas Gerais

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André Ag Faraco

Universidade Federal de Minas Gerais

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Eduardo Antonio Ferraz Coelho

Universidade Federal de Minas Gerais

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Lourena E. Costa

Universidade Federal de Minas Gerais

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