Judith A. Kapp

STIMULATION OF ANTIBODY RESPONSES IN VITRO BY ANTIGEN-BEARING SYNGENEIC AND ALLOGENEIC MACROPHAGES

Abstract The ability of antigen-bearing syngeneic and allogeneic peptone-induced peritoneal exudate macrophages to support development of primary and secondary antibody responses to a T cell-dependent antigen by murine lymphoid cells in vitro has been investigated. Syngeneic and allogeneic macrophages support development of comparable primary antibody responses. Immunized spleen cells, however, develop secondary antibody responses preferentially when stimulated in vitro with antigen on macrophages syngeneic to the macrophages used to immunize the spleen cells in vivo . The genetic restrictions governing effective macrophage-lymphoid cell interactions in secondary antibody responses appear to be operative at the level of the immunized T cell. The implications that sensitized T cells selectively recognize antigen presented in the context of the macrophage membrane-antigen complex which sensitized the T cells initially are considered.

STIMULATION OF SPECIFIC SUPPRESSOR T CELLS BY THE TERPOLYMER L-GLUTAMIC ACID60-L-ALANINE30-L-TYROSINE10 IN GENETIC NONRESPONDER MICE

Publisher Summary This chapter presents a study in which specific suppressor T cells is stimulated by the terpolymer L-glutamic acid60-L-alanine30-L-tyrsine10 (GAT) in genetic nonresponder mice. The antibody responses to both GAT and GAT-MBSA are T cell-dependent. Furthermore, spleen cells from GAT-primed, irradiated responder mice are able to provide GAT-specific helper T cell function for B cell responses to GAT, while no GAT-specific helper T cell function could be demonstrated in spleen cells from GAT-primed, nonresponder mice. These data suggested that the defect in genetic nonresponder mice is indeed the failure of their T cells, after interaction with GAT, to provide appropriate helper T cell function for the initiation of the B cell response to GAT. The antibody response to GAT in vitro provides, therefore, a suitable system to explore the possibility that GAT-specific suppressor T cells are present and function in nonresponder animals.

SUPPRESSIVE ACTIVITY OF LYMPHOID CELL EXTRACTS FROM NON-RESPONDER MICE INJECTED WITH THE TERPOLYMER L-GLUTAMIC ACID60 – L-ALANINE30-L-TYROSINE10 (GAT)

ABSTRACT The immune response by inbred strains of mice to the terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) is under H-2 linked genetic control. In non-responder mice, GAT does not elicit a GAT-specific antibody response but does stimulate development of a population of GAT-specific suppressor T cells. Furthermore, extracts prepared from lymphoid cells of GAT-primed non-responder mice inhibit the development of GAT-specific antibody responses to GAT coupled methylated bovine serum albumin (GAT-MBSA) by normal non-responder syngeneic mice. This suppression is specific and dose-dependent. The activity can be removed from these extracts by passage over GAT-Sepharose, but not BSA-Sepharose. The molecular weight of the active component(s) is between 10,000–50,000.