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Dive into the research topics where Judith M. Nocek is active.

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Featured researches published by Judith M. Nocek.


Journal of Physical Chemistry B | 2008

Kinetic-dynamic model for conformational control of an electron transfer photocycle: mixed-metal hemoglobin hybrids.

Ami D. Patel; Judith M. Nocek; Brian M. Hoffman

It is becoming increasingly clear that the transfer of an electron across a protein-protein interface is coupled to the dynamics of conformational conversion between and within ensembles of interface conformations. Electron transfer (ET) reactions in conformationally mobile systems provide a clock against which the rapidity of a dynamic process may be measured, and we here report a simple kinetic (master equation) model that self-consistently incorporates conformational dynamics into an ET photocycle comprised of a photoinitiated forward step and thermal return to ground. This kinetic/dynamic (KD) model assumes an ET complex exists as multiple interconverting conformations which partition into an ET-optimized (reactive; R) population and a less-reactive population ( S). We take the members of each population to be equivalent by constraining them to have the same conformational energy, the same average rate constant for conversion to members of the other population, and the same rate constants for forward and back ET. The result is a mapping of a complicated energy surface onto the simple gating, two-well surface, but with rate constants that are defined microscopically. This model successfully describes the changes in the ET photocycle within the predocked mixed-metal hemoglobin (Hb) hybrid, [alpha(Zn), beta(Fe3+N 3 (-))], as conformational kinetics are modulated by variations in viscosity (eta = 1-15 cP; 20 degrees C). The description reveals how the conformational routes by which a hybrid progresses through a photocycle differ in different dynamic regimes. Even at eta = 1 cP, the populations are not in fast exchange, and ET involves a complex interplay between conformational and ET processes; at intermediate viscosities the hybrid exhibits differential dynamics in which the forward and back ET processes involve different initial ensembles of configurational substates; by eta = 15 cP, the slow-exchange limit is approached. Even at low viscosity, the ET-coupled motions are fairly slow, with rate constants of <10 (3) s (-1). Current ideas about Hb function lead to the testable hypothesis that ET in the hybrid may be coupled to allosteric fluctuations of the two [alpha 1, beta 2] dimers of Hb.


Journal of the American Chemical Society | 1986

1H NMR probes of the binuclear iron cluster in hemerythrin

Michael J. Maroney; Donald M. Kurtz; Judith M. Nocek; Linda L. Pearce; Lawrence Que


Journal of the American Chemical Society | 1991

Multiphasic intracomplex electron transfer from cytochrome c to zinc cytochrome c peroxidase: conformational control of reactivity

Sten A. Wallin; Eric D. A. Stemp; Andrew M. Everest; Judith M. Nocek; Thomas L. Netzel; Brian M. Hoffman


Journal of the American Chemical Society | 1995

19F and 1,2H ENDOR Study of Distal-Pocket N(.epsilon.)-H.cntdot. .cntdot. .cntdot.F Hydrogen Bonding in Fluorometmyoglobin

Yang-cheng Fann; Jui-lin Ong; Judith M. Nocek; Brian M. Hoffman


Journal of the American Chemical Society | 1985

Nitric oxide adduct of the binuclear iron center in deoxyhemerythrin from Phascolopsis gouldii. Analogue of a putative intermediate in the oxygenation reaction

Judith M. Nocek; Donald M. Kurtz; J.Timothy Sage; Peter G. Debrunner; Michael J. Maroney; Lawrence Que


Journal of the American Chemical Society | 2006

NMR Spectroscopy Can Characterize Proteins Encapsulated in a Sol-Gel Matrix

Korin E. Wheeler; Judith M. Nocek; Brian M. Hoffman


Journal of Inorganic Biochemistry | 1989

Structural basis for conformational gating of electron transfer in zinc-cytochrome c peroxidase/cytochrome c complexes

A.G. Mauk; Eric D. A. Stemp; Judith M. Nocek; E. Margoliash; Brian M. Hoffman


Archive | 2003

An Approach to NMR Treatment of Structural Perturbations in Paramagnetic Proteins too Big for Solution Structure Determination

Judith M. Nocek; Kai Huang; Brian M. Hoffman


Journal of Inorganic Biochemistry | 1995

Cyclic electron transfer within the 1:1 [Zn-deutero-myoglobin, cytochrome B5] complex and the 2:1 [cytochrome C, cytochrome C peroxidase] complex

Judith M. Nocek; Jian S. Zhou; Michael L. Devan; Bhavini P. Sishta; Janelle R. Casey; A. Grant Mauk; Brian M. Hoffman


Journal of Inorganic Biochemistry | 1993

Evidence for non-redox quenching of zinc-cytochrome C peroxidase by Fe3+ cytochrome C.

Michael L. Devan; Judith M. Nocek; A.G. Mauk; B.N. Hoffman

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Eric D. A. Stemp

California Institute of Technology

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Donald M. Kurtz

University of Texas at San Antonio

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Lawrence Que

University of Minnesota

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Michael J. Maroney

University of Massachusetts Amherst

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Ami D. Patel

Northwestern University

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Jian S. Zhou

Northwestern University

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Kai Huang

Northwestern University

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