Judy A. Johnson

Dielectric properties of fruits and insect pests as related to radio frequency and microwave treatments.

Information on dielectric properties of commodities and insect pests is needed in developing thermal treatments for postharvest insect control based on radio frequency (RF) and microwave energy. Dielectric properties of six commodities along with four associated insect pests were measured between 1 and 1800 MHz using an open-ended coaxial-line probe technique and at temperatures between 20 and 608C. The dielectric loss factor of fresh fruits and insects decreased with increasing frequency at constant temperatures. The loss factor of fresh fruits and insects increased almost linearly with increasing temperature at 27 MHz radio frequency, but remained nearly constant at 915 MHz microwave frequency. Both dielectric constant and loss factor of nuts were very low compared to those of fresh fruits and insects. The temperature effect on dielectric properties of nuts was not significant at 27 MHz. The large difference in the loss factor between insects and nuts at 27 MHz suggests possible differential heating of insects in nuts when treated at the same time in a RF system.

Process protocols based on radio frequency energy to control field and storage pests in in-shell walnuts

A practical process protocol was developed to control insect pests in in-shell walnuts using a 27 MHz pilot scale radio frequency (RF) system. Fifth-instars, that had been determined to be the most heat resistant life stage for navel orangeworm (Amyelois transitella [Walker]) using a heating block system, were selected as the targeted insect in the protocol development. RF heating to 55 °C and holding in hot air for at least 5 min resulted in 100% mortality of the fifth-instar navel orangeworm. Rancidity, sensory qualities and shell characteristics were not affected by the treatments. The process slightly reduced the moisture content of the walnut kernels, which could prove an additional benefit by providing even nut moisture content and reducing the growth of microorganisms. If this method can be economically integrated into the handling process, it should have excellent potential as a disinfestation method for in-shell walnuts.

Thermal-death kinetics of fifth-instar Amyelois transitella (Walker) (Lepidoptera: Pyralidae)

Information on kinetics for thermal mortality of navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), is needed for developing post-harvest phytosanitation thermal treatments of walnuts. Thermal-death kinetics for fifth-instar navel orangeworms were determined at temperatures between 461C and 541C at a heating rate of 181C min � 1 using a heating block system. Thermal-death curves for fifth-instar navel orangeworms followed a 0.5th-order of kinetic reaction. The time required to achieve 100% mortality (N0 ¼ 600) decreased with increasing temperature in a logarithmic manner. Complete kill of 600 insects required a minimum exposure time of 140, 50, 15, 6, and 1 min at 461C, 481C, 501C, 521C, and 541C, respectively. The reaction rate (k) was affected by treatment temperatures following an Arrhenius relationship. The activation energy for thermal kill of fifth-instar navel orangeworms was

Thermal Death Kinetics of Red Flour Beetle (Coleoptera: Tenebrionidae)

While developing radio frequency heat treatments for dried fruits and nuts, we used a heating block system developed by Washington State University to identify the most heat-tolerant life stage of red flour beetle, Tribolium castaneum (Herbst), and to determine its thermal death kinetics. Using a heating rate of 15 degrees C/min to approximate the rapid heating of radio frequency treatments, the relative heat tolerance of red flour beetle stages was found to be older larvae > pupae and adults > eggs and younger larvae. Lethal exposure times for temperatures of 48, 50, and 52 degrees C for the most heat-tolerant larval stage were estimated using a 0.5th order kinetic model. Exposures needed for 95% mortality at 48 degrees C were too long to be practical (67 min), but increasing treatment temperatures to 50 and 52 degrees C resulted in more useful exposure times of 8 and 1.3 min, respectively. Red flour beetle was more sensitive to changes in treatment temperature than previously studied moth species, resulting in red flour beetle being the most heat-tolerant species at 48 degrees C, but navel orangeworm, Amyelois transitella (Walker), being most heat tolerant at 50 and 52 degrees C. Consequently, efficacious treatments for navel orangeworm at 50-52 degrees C also would control red flour beetle.

Seasonal Occurrence of Postharvest Dried Fruit Insects and Their Parasitoids in a Culled Fig Warehouse

Abstract Parasitoids of dried-fruit insects were surveyed at a culled fig warehouse in Fresno, CA. Three parasitoids of pyralid larvae were found: Habrobracon hebetor (Say), Venturia canescens (Gravenhorst), and at least one species in the genus Goniozus Förster. Two parasitoids of pyralid pupae also were noted: Mesostenus gracilis (Cresson) and a new species of Psilochalcis Keifer. The latter is a new host association. Several beetle parasitoids were present, including Anisopteromalus calandrae (Howard), three species of Cephalonomia Westwood, Laelius centratus (Say), and Cerchysiella utilis Noyes. C. utilis, a parasitoid of driedfruit beetle, Carpophilus hemipterus (L.), is a new record for California. Most activity by parasitoids (detected by yellow flight traps) occurred directly above the fig mass. Pyralid parasitoids exhibited two peaks of seasonal activity; one in late summer through early fall, shortly after new figs were brought into the warehouse, and one in the spring. H. hebetor generally attacked older host larvae, whereas V. canescens equally attacked older and younger larvae, indicating that these two parasitoids may coexist by exploiting different portions of the host population. H. hebetor was active throughout the winter, suggesting that winter release of H. hebetor could be used to control diapausing pyralid populations in dried fruit and nut storage areas.

Integration of nonchemical treatments for control of postharvest pyralid moths (Lepidoptera: Pyralidae) in almonds and raisins.

Abstract We propose a treatment strategy combining an initial disinfestation treatment with one of three protective treatments as an alternative for chemical fumigation of almonds and raisins for control of postharvest insect populations. Initial disinfestation treatments using low oxygen controlled atmosphere (0.4% O2) were designed to disinfest product of field populations of pyralid moths; navel orangeworm, Amyelois transitella (Walker), in almonds and raisin moth, Cadra figulilella (Gregson), in raisins. The protective treatments were cold storage (10°C), controlled atmosphere (5% O2) storage, and application of the Indianmeal moth granulosis virus, and were designed to prevent establishment of Indianmeal moth, Plodia interpunctella (Hübner). The initial disinfestation treatment was effective against laboratory populations of navel orangeworm and raisin moth. Efficacy of protective treatments was determined by exposure of commodities to laboratory Indianmeal moth populations at levels far higher than those found in commercial storage facilities. All three protective treatments prevented development of damaging Indianmeal moth populations as measured by pheromone trap catches and evaluation of product samples. Quality analysis by commercial laboratories showed that overall product quality for all protective treatments was maintained at levels acceptable by industry standards.

Survival of Indianmeal Moth and Navel Orangeworm (Lepidoptera: Pyralidae) at Low Temperatures

Abstract Concerns over insect resistance, regulatory action, and the needs of organic processors have generated renewed interest in developing nonchemical alternative postharvest treatments to fumigants used on dried fruits and nuts. Low-temperature storage has been identified as one alternative for the Indianmeal moth, Plodia interpunctella (Hübner), and navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), common postharvest pests in California dried fruits and nuts. The response of eggs, nondiapausing larvae, and pupae of both species to exposure to low temperatures (0, 5, and 10°C) was evaluated. Eggs of both species were the least tolerant of low temperatures. At 0 and 5°C, pupae were most tolerant, but at 10°C, nondiapausing larvae of both species were most tolerant, with lethal time (LT)95 values of 127 and 100 d for Indianmeal moth and navel orangeworm, respectively. The response of diapausing Indianmeal moth larvae to subfreezing temperatures also was evaluated. Diapausing larvae were very cold tolerant at −10°C, with LT95 values of 20 and 17 d for long-term laboratory and recently isolated cultures, respectively. Diapausing larvae were far less tolerant at lower temperatures. At −15°C, LT95 values for both cultures were <23 h, and at −20°C, LT95 values were <7 h. Refrigeration temperatures of 0–5°C should be useful in disinfesting product contaminated with nondiapausing insects, with storage times of 3 wk needed for adequate control. Relatively brief storage in commercial freezers, provided that the temperature throughout the product was below −15°C for at least 48 h, also shows potential as a disinfestation treatment, and it is necessary when diapausing Indianmeal moth larvae are present.

Effect of relative humidity and product moisture on response of diapausing and nondiapausing Indianmeal moth (Lepidoptera: Pyralidae) larvae to low pressure treatments.

ABSTRACT Low pressure treatment in flexible polyvinyl chloride containers is a potential alternative to chemical fumigants for California tree nuts. Laboratory studies investigated the effect of relative humidity and product moisture on weight loss and mortality of diapausing and nondiapausing larvae of the Indianmeal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae), exposed to 50 mmHg. Diapausing larvae were far more tolerant than nondiapausing larvae to low pressure; exposure times nearly twice those of nondiapausing larvae were required to obtain comparable weight loss or mortality levels in diapausing larvae. Relative humidity was found to have a large effect on both weight loss (assumed to be due to moisture loss) and mortality of both nondiapausing and diapausing larvae. Mortality and weight loss increased as humidity levels decreased. By controlling the relative humidity of the treatment chamber, product moisture also strongly affected weight loss and mortality. The results suggest that for tree nuts, product moisture levels may affect the efficacy of low pressure treatments.

Response of Postharvest Tree Nut Lepidopteran Pests to Vacuum Treatments

ABSTRACT Industry concerns over insect resistance, regulatory action, and the needs of organic processors have renewed interest in nonchemical alternative postharvest treatments to fumigants used for California tree nuts. The development of inexpensive polyvinyl chloride containers capable of holding low pressures has increased the practicality of vacuum treatments for durable commodities such as tree nuts. To develop vacuum treatment protocols, we determined the relative tolerance to vacuum (50 mmHg) at 25 and 30°C of different life stages of three postharvest pests of tree nuts: codling moth, Cydia pomonella (L.), navel orangeworm, Amyelois transitelle (Walker), and Indianmeal moth, Plodia interpunctella (Hübner). At both temperatures, nondiapausing codling moth larvae were the least tolerant stage tested. LT95 values for diapausing Indianmeal moth larvae were similar to Indianmeal moth eggs at both temperatures. Indianmeal moth diapausing larvae and eggs were the most tolerant at 25°C, whereas navel orangeworm eggs were most tolerant at 30°C. Field tests using GrainPro Cocoons (GrainPro, Inc., Concord, MA) to treat shelled almonds, Prunus dulcis (Mill.) D.A. Webb, in bins at vacuum levels of 18–43 mmHg at average winter temperatures (6–10°C) showed that diapausing codling moth larvae were the most tolerant under these conditions and that exposures of 7–13 d provided incomplete control. Summer field tests treating in-shell almonds in bags at average temperatures of 25–30°C provided complete control with 48 h exposure to average vacuum levels of 50 mmHg, and navel orangeworm eggs were the most tolerant stage.

Thermal Death Kinetics of Conogethes punctiferalis (Lepidoptera: Pyralidae) as Influenced by Heating Rate and Life Stage

ABSTRACT Thermal death kinetics of Conogethes punctiferalis (Guenée) (Lepidoptera: Pyralidae) at different life stages, heating rate, and temperature is essential for developing postharvest treatments to control pests in chestnuts. Using a heating block system (HBS), the most heat-tolerant life stage of C. punctiferalis and the effects of heating rate (0.1, 0.5, 1, 5, and 10°C/min) on insect mortality were determined. The thermal death kinetic data of fifth-instar C. punctiferalis were obtained at temperatures between 44 and 50°C at a heating rate of 5°C/min. The results showed that the relative heat tolerance of C. punctiferalis was found to be fifth instars > pupae > third instars > eggs. To avoid the enhanced thermal tolerance of C. punctiferalis at low heating rates (0.1 or 0.5°C/min), a high heating rate of 5°C/min was selected to simulate the fast radio frequency heating in chestnuts and further determine the thermal death kinetic data. Thermal death curves of C. punctiferalis followed a 0th-order kinetic reaction model. The minimum exposure time to achieve 100% mortality was 55, 12, 6, and 3 min at 44, 46, 48, and 50°C, respectively. The activation energy for controlling C. punctiferalis was 482.15 kJ/mol with the z value of 4.09°C obtained from the thermal death—time curve. The information provided by thermal death kinetics for C. punctiferalis is useful in developing effective postharvest thermal treatment protocols for disinfesting chestnuts.