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Dive into the research topics where Juergen Spinke is active.

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Featured researches published by Juergen Spinke.


Clinical Chemistry and Laboratory Medicine | 2017

A new immunochemistry platform for a guideline-compliant cardiac troponin T assay at the point of care: proof of principle

Eloisa Lopez-Calle; Pamela Espindola; Juergen Spinke; Sascha Lutz; Alfons Nichtl; Armin Tgetgel; Nicolas Herbert; Moritz Marcinowski; Juergen Klepp; Thomas Fischer; Thorsten Brueckner; Christoph Boehm; Thomas Keller

Abstract Background: A multitude of troponin assays for the point-of-care (POC) have been developed showing a lack of analytical sensitivity and precision. We present a new platform solution for the high-sensitivity detection of cardiac troponin T (cTnT) in a 30 μL whole blood sample with a turnaround time of 11 min. Methods: The immunoassay was completely run in a ready-to-use plastic disposable, a centrifugal microfluidic disc with fully integrated reagents. After the sample application, the assay was automatically processed by separating the cellular blood components via centrifugation, followed by incubation of a defined volume from the generated plasma with the immunoreagents. The fluorescence in the signal zone of a membrane was measured after its washing for the cTnT quantitation. Results: A calibration curve, measured in whole blood samples spiked with native human cTnT, was generated covering a range up to a concentration of approximately 8300 ng/L. The lower detection limit was determined to be 3.0 ng/L. At a concentration of 14 ng/L, the 99th percentile value from the high-sensitivity cardiac troponin T (hs-cTnT) assay in the Elecsys® system, the imprecision (CV) was 3.8%. A CV profile indicated that the functional sensitivity for a CV <10% was 6.8 ng/L. The assay did not show any significant cross-reaction with human skeletal troponin T. We observed an excellent correlation with the hs-TnT Elecsys® assay for 49 clinical plasma samples (r=0.9744). Conclusions: The described technology shows that an analytical performance for a highly sensitive determination of cTnT can be achieved in a POC setting.


Archive | 2009

Rotatable test element

Christoph Boehm; Norbert Oranth; Juergen Spinke


Archive | 2004

Nanoparticles for optical sensors

Valerie Desprez; Norbert Oranth; Juergen Spinke; James Kenneth Tusa


Archive | 2006

Method for Increasing the Dynamic Measuring Range of Test Elements Based on Specific Binding Reactions

Ludger Bodenbach; Juergen Schaeffler; Juergen Spinke


Archive | 2004

Analytical sandwich test for determining NT-proBNP

Juergen Spinke; Alfons Nichtl; Volker Klemt; Klaus Hallermayer; Michael Grol; Anneliese Borgya; Andreas Gallusser


Archive | 2004

Analytical sandwich assay for the determination of NT-proBNP

Anneliese Borgya; Andreas Gallusser; Michael Grol; Klaus Hallermayer; Volker Klemt; Alfons Nichtl; Juergen Spinke


Archive | 2008

Reagent for fluorimetric determination of analytes

Carina Horn; Hans-Peter Josel; Juergen Spinke; Rupert Herrmann; Dieter Heindl


Archive | 2017

ROTATABLE CARTRIDGE WITH A METERING CHAMBER FOR ANALYZING A BIOLOGICAL SAMPLE

Christoph Boehm; Sascha Lutz; Juergen Spinke


Archive | 2015

ROTATABLE CARTRIDGE FOR PROCESSING AND ANALYZING A BIOLOGICAL SAMPLE

Christoph Boehm; Sascha Lutz; Juergen Spinke


Archive | 2005

Method for increasing the dynamic measuring range of test elements, especially immunological test elements, that are based on specific bonding reactions

Juergen Spinke; Juergen Schaeffler; Ludger Bodenbach

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