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Dive into the research topics where Jukka Melkko is active.

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Featured researches published by Jukka Melkko.


Journal of the American College of Cardiology | 2002

Progression of human aortic valve stenosis is associated with tenascin-C expression

Jari Satta; Jukka Melkko; Raimo Pöllänen; Juha Tuukkanen; Paavo Pääkkö; Pasi Ohtonen; Ari Mennander; Ylermi Soini

OBJECTIVESnWe sought to assess tenascin-C (TN-C) expression and its possible pathobiological impact in human aortic valve stenosis.nnnBACKGROUNDnTenascin-C, a large extracellular matrix glycoprotein, has lately been increasingly connected to cardiovascular pathologies. As TN-C is a multifunctional protein implicated in cell proliferation, migration and differentiation, we investigated the pattern of its expression in diseased human aortic valves.nnnMETHODSnFifty-five tricuspid, non-rheumatic stenotic aortic valves were collected from patients undergoing aortic valve replacement, and the controls consisted of four normal valves from individuals who had suffered traumatic death and one from a patient operated on because of a noncalcified purely regurgitant valve. A monoclonal mouse antibody to human TN-C (143DB7) was used as the primary antibody in immunostaining. To study the source of TN-C messenger RNA synthesis, some tissue samples were also examined using in situ hybridization. In order to identify smooth muscle cell differentiation, commercially available antibodies against alpha-smooth muscle actin were used, and immunophenotypic analysis of inflammatory cells was carried out by using the monoclonal mouse antibodies UCHL-1, L26 and PGM-1.nnnRESULTSnIn normal valves, TN-C expression was associated with the basement membrane beneath the endothelial cells, whereas stenotic valves showed no such expression but rather immunoreactivity in the deeper layers of the valves. This reactivity was associated with the characteristics typical of the stenosing process and the increased mechanical loading caused by hypertension.nnnCONCLUSIONSnWe hypothesize that the overexpression of TN-C in stenotic human aortic valves may emphasize that this disease is an active rather than a degenerative process.


Cytotherapy | 2010

Attempt to treat congenital pseudarthrosis of the tibia with mesenchymal stromal cell transplantation

Jonne Tikkanen; Hannu-Ville Leskelä; Siri Lehtonen; Vesa Vähäsarja; Jukka Melkko; Lauri Ahvenjärvi; Eija Pääkkö; Kalervo Väänänen; Petri Lehenkari

BACKGROUND AIMSnCongenital pseudarthrosis of the tibia (CPT) caused by neurofibromatosis type 1 (NF1) is a refractory disease occurring in childhood. We present two cases that had failed all earlier treatment attempts and, as a last treatment attempt, the patients were chosen to receive mesenchymal stromal cell (MSC) transplantation prior to amputation.nnnMETHODSnThe MSC from bone marrow (BM) were harvested from the iliac crest and cultured in osteoinductive medium for 3 weeks. The cultured MSC were injected in solution into BM canals of the tibia and around the resection line or bone defect in a 3-dimensional collagen sponge scaffold. After the MSC transplantation, the patients were monitored during a 10-month follow-up period. In both cases, bone formation at the pseudarthrosis site was observed and two of three treated bone defects healed. For clinical reasons not related to cell transplantation, such as new infection and pseudarthrosis and severe shortening of the leg, both extremities were finally amputated and bone samples were analyzed to evaluate MSC therapy effect and safety.nnnRESULTSnMSC transplantation normalized bone remodeling, promoted bone resorption and improved the overall structure of bone. The number of osteoclasts in the cortical bone was 2-fold higher compared with the monitored situation before MSC transfer. In addition, the mineral content of the bone improved after transplantation. We could see no sign of aberrant bone formation or malignant transformation.nnnCONCLUSIONSnOur data suggest that MSC transplantation is a possibility for treatment of CPT caused by NF1 in less severe cases without adjunct defects.


Arthritis Research & Therapy | 2016

Rheumatoid arthritis antigens homocitrulline and citrulline are generated by local myeloperoxidase and peptidyl arginine deiminases 2, 3 and 4 in rheumatoid nodule and synovial tissue

Sanna Turunen; Johanna Huhtakangas; Tomi Nousiainen; Maarit Valkealahti; Jukka Melkko; Juha Risteli; Petri Lehenkari

BackgroundSeropositive rheumatoid arthritis (RA) is characterized by autoantibodies binding to citrullinated and homocitrullinated proteins. We wanted to study the expression patterns of these disease-associated protein forms and if the rheumatoid nodule and synovial tissue itself contain biologically active levels of citrullinating peptidyl arginine deiminases 2, 3 and 4 and homocitrullination-facilitating neutrophil enzyme myeloperoxidase.MethodTotal of 195 synovial samples from metatarsal joints from five ACPA/RF-positive RA patients (nu2009=u200977), synovial samples from knees of eight seropositive RA (nu2009=u200960), seven seronegative RA (nu2009=u200933) and five osteoarthritis (nu2009=u200925) patients were analyzed for citrulline and homocitrulline contents using HPLC. The location of citrulline- and homocitrulline-containing proteins, PAD 2, 3, 4 and myeloperoxidase were shown by immunostaining. Myeloperoxidase and citrulline- or homocitrulline-containing proteins were stained on Western blot.ResultsOverall, necrosis was frequent in metatarsals of seropositive RA and absent in seronegative RA and osteoarthritis patients. In histological analysis, there was a significant local patterning and variation in the citrulline and homocitrulline content and it was highest in metatarsal synovial tissues of seropositive RA patients. We found peptidyl arginine deiminase 2, 3 and 4 in the lining and sublining layers of intact synovial tissue. Myeloperoxidase was found locally around necrotic areas. The tissues with necrosis contained the highest levels of citrulline and homocitrulline.ConclusionsRheumatoid nodules and synovia contain significant amount of PAD2, 3 and 4 and myeloperoxidase enzymes. These enzymes could explain the levels of citrulline and homocitrulline in seropositive RA synovial and rheumatoid nodule tissues especially around necrotic tissue.


Histology and Histopathology | 2009

Tenascin-C and type I and III collagen expression in total Achilles tendon rupture. An immunohistochemical study

Ari Pajala; Jukka Melkko; Juhana Leppilahti; Pasi Ohtonen; Ylermi Soini; Juha Risteli


Anticancer Research | 2004

Matrix metalloproteinase-9 (MMP-9) immunoreactive protein has modest prognostic value in locally advanced breast carcinoma patients treated with an adjuvant antiestrogen therapy.

Eeva Rahko; Arja Jukkola; Jukka Melkko; Pääkkö Paavo; Risto Bloigu; Anne Talvensaari-Mattila; Taina Turpeenniemi-Hujanen


Archive | 1991

Method for the immunological determination of the carboxyterminal propeptide of type I procollagen

Juha Risteli; Leila Risteli; Jukka Melkko


Anticancer Research | 2001

Aminoterminal propeptide of the alpha1-homotrimer variant of human type I procollagen (hotPINP) in malignant pleural effusion.

Saila Kauppila; Arja Jukkola; Jukka Melkko; Leila Risteli; Taina Turpeeniemi-Hujanen; Klaus Vuorinen; Juha Risteli


Archive | 2002

Biomarkers of Disease: Biomarkers of bone formation

Juha Risteli; Saila Kauppila; Arja Jukkola; Eevastiina Marjoniemi; Jukka Melkko; Leila Risteli


Archive | 1998

Antibody to aminoterminal propeptide of type 1 procollagen

Juha Risteli; Leila Risteli; Jukka Melkko; Saila Kauppila


Archive | 1996

Antibody to aminoterminal propeptide of type 1 procollagen, and assay method using it

Juha Risteli; Leila Risteli; Jukka Melkko; Saila Kauppila

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Arja Jukkola

Oulu University Hospital

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Pasi Ohtonen

Oulu University Hospital

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Ylermi Soini

University of Eastern Finland

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