Julia P. O'Neil
Monsanto
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Publication
Featured researches published by Julia P. O'Neil.
Gene | 1996
Wesley C. Warren; Kristin A. Bentle; Michael R. Schlittler; Aimee C. Schwane; Julia P. O'Neil; Gregg Bogosian
In Escherichia coli and most other microorganisms, peptide synthesis is started at methionine start codons which are read only by N-formyl-methionine-tRNA. The formyl group is normally removed from the N-terminal Met residue of the peptide by peptide deformylase (PDF). However, it has been observed that overproduction of proteins in recombinant bacteria often yields protein products which are incompletely deformylated. Certain proteins could be poor substrates for PDF and exhibit incomplete deformylation, particularly when they are overproduced. Strains of E. coli which overproduce bovine somatotropin (BST) have a significant fraction of the BST with the formyl group retained. The PDF gene was isolated and positioned into a BST production vector in such a way that the BST and PDF genes were coexpressed. In strains containing this coexpression vector, the levels of PDF were increased and formylated BST was undetectable.
Applied and Environmental Microbiology | 1996
Gregg Bogosian; Laura E Sammons; Patricia J. L. Morris; Julia P. O'Neil; M A Heitkamp; Deborah B. Weber
Journal of Bacteriology | 2000
Gregg Bogosian; Noelle D. Aardema; Edward V. Bourneuf; Patricia J. L. Morris; Julia P. O'Neil
Applied and Environmental Microbiology | 1998
Gregg Bogosian; Patricia J. L. Morris; Julia P. O'Neil
Archive | 2000
Gregg Bogosian; Julia P. O'Neil; Katherine C Terlesky
Gene | 1993
Gregg Bogosian; Kristin Bilyeu; Julia P. O'Neil
Archive | 2004
Gregg Bogosian; Julia P. O'Neil; Hong Q. Smith
Archive | 2006
Gregg Bogosian; Julia P. O'Neil; Hong Q. Smith
Archive | 1999
Gregg Bogosian; Julia P. O'Neil; Jeffrey M. Staub
Archive | 2002
Gregg Bogosian; Julia P. O'Neil; Noelle D. Aardema