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Dive into the research topics where Julia P. O'Neil is active.

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Featured researches published by Julia P. O'Neil.


Gene | 1996

Increased production of peptide deformylase eliminates retention of formylmethionine in bovine somatotropin overproduced in Escherichia coli

Wesley C. Warren; Kristin A. Bentle; Michael R. Schlittler; Aimee C. Schwane; Julia P. O'Neil; Gregg Bogosian

In Escherichia coli and most other microorganisms, peptide synthesis is started at methionine start codons which are read only by N-formyl-methionine-tRNA. The formyl group is normally removed from the N-terminal Met residue of the peptide by peptide deformylase (PDF). However, it has been observed that overproduction of proteins in recombinant bacteria often yields protein products which are incompletely deformylated. Certain proteins could be poor substrates for PDF and exhibit incomplete deformylation, particularly when they are overproduced. Strains of E. coli which overproduce bovine somatotropin (BST) have a significant fraction of the BST with the formyl group retained. The PDF gene was isolated and positioned into a BST production vector in such a way that the BST and PDF genes were coexpressed. In strains containing this coexpression vector, the levels of PDF were increased and formylated BST was undetectable.


Applied and Environmental Microbiology | 1996

Death of the Escherichia coli K-12 strain W3110 in soil and water

Gregg Bogosian; Laura E Sammons; Patricia J. L. Morris; Julia P. O'Neil; M A Heitkamp; Deborah B. Weber


Journal of Bacteriology | 2000

Recovery of Hydrogen Peroxide-Sensitive Culturable Cells of Vibrio vulnificus Gives the Appearance of Resuscitation from a Viable but Nonculturable State

Gregg Bogosian; Noelle D. Aardema; Edward V. Bourneuf; Patricia J. L. Morris; Julia P. O'Neil


Applied and Environmental Microbiology | 1998

A Mixed Culture Recovery Method Indicates that Enteric Bacteria Do Not Enter the Viable but Nonculturable State

Gregg Bogosian; Patricia J. L. Morris; Julia P. O'Neil


Archive | 2000

DNA construct for regulating the expression of a polypeptide coding sequence in a transformed bacterial host cell

Gregg Bogosian; Julia P. O'Neil; Katherine C Terlesky


Gene | 1993

Genome rearrangements by residual S10 elements in strains of Escherichia coli K-12 which had undergone Tn70 mutagenesis and fusaric acid selection

Gregg Bogosian; Kristin Bilyeu; Julia P. O'Neil


Archive | 2004

Prevention of incorporation of non-standard amino acids into protein

Gregg Bogosian; Julia P. O'Neil; Hong Q. Smith


Archive | 2006

Hybrid portable origin of replication plasmids

Gregg Bogosian; Julia P. O'Neil; Hong Q. Smith


Archive | 1999

Bacterial expression systems based on plastic or mitochondrial promoter combinations

Gregg Bogosian; Julia P. O'Neil; Jeffrey M. Staub


Archive | 2002

Recombinant DNA vectors for expression of somatotropins

Gregg Bogosian; Julia P. O'Neil; Noelle D. Aardema

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