Juliana Nascimento
University of São Paulo
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Featured researches published by Juliana Nascimento.
Theriogenology | 2012
André Furugen Cesar de Andrade; Fabiane Gilli Zaffalon; Eneiva Carla Carvalho Celeghini; Juliana Nascimento; F. F. Bressan; Simone Maria Massami Kitamura Martins; Rubens Paes de Arruda
The objective was to verify the relationship between equine semen cryopreservation and changes related to increased lipid peroxidation. Also, addition of autologous or homologous seminal plasma from a stallion with a good freezing response to post-thawed sperm was tested to determine whether it would confer protection. Frozen-thawed sperm were evaluated and allocated into three groups: without plasma addition, and supplemented with either homologous or autologous seminal plasma. All groups were evaluated at 0, 60 and 120 min after incubation at 37 °C. Cryopreservation did not increase plasma membrane disorders (mean ± SEM 9.48 ± 0.65 and 1.62 ± 0.23% in raw and frozen-thawed sperm, respectively). However, both membrane peroxidation and protein phosphorylation were increased (P < 0.05) compared to raw semen (1.74 and 5.20-fold, respectively). There was a correlation (r = 0.73; P < 0.05) between the increase in lipid peroxidation and tyrosine phosphorylation. Seminal plasma, regardless of origin, reduced (P > 0.05) tyrosine phosphorylation present on the surface of cryopreserved sperm; however, lipid peroxidation was not significantly reduced. In conclusion, we inferred that emergence of phosphorylated proteins on the surface of cryopreserved sperm was due to increased lipid peroxidation that occurred during the freezing/thawing process; however, reduced tyrosine phosphorylation that occurred after addition of seminal plasma was triggered by other mechanisms, apparently independent from the reduction in membrane peroxidation.
Reproduction in Domestic Animals | 2011
Afc De Andrade; Fabiane Gilli Zaffalon; E. C. C. Celeghini; Juliana Nascimento; Ofb Tarragó; S. M. M. K. Martins; Maria Augusta Alonso; Rubens Paes de Arruda
Effect of seminal plasma addition after thawing on viability or cryocapacitation is not definitively established. This experiment was performed to verify the effect of adding seminal plasma, autologous or homologous (from an animal with good semen freezability). Five ejaculates from each of four stallions with proven fertility were collected and cryopreserved. The semen was subsequently thawed and divided into the following three treatment groups: no seminal plasma addition after semen thawing (NOSP); the addition of homologous seminal plasma after semen thawing (HSP) and the addition of autologous seminal plasma after semen thawing (ASP). The addition of 20% of seminal plasma led to an increase in the cell population that simultaneously show plasma and acrosomal membrane integrity (p < 0.05). The addition of seminal plasma did not alter the total motility, the amount of cells with mitochondrial membrane potential or the sperm velocities (average path velocity, straight-line velocity and curvilinear velocity). However, the beat/cross-frequency, straightness and linearity were reduced in ASP and HSP groups (p < 0.05). Unexpectedly, the addition of homologous seminal plasma reduced the proportion of cells with progressive motility (p < 0.05) and the addition of autologous seminal plasma reduced the amplitude of the lateral head displacement (p < 0.05). Based on the increase in the cell populations that had the plasma and acrosomal membrane integrity simultaneously identified in this study, we proposed that the addition of seminal plasma (autologous or homologous) into post-thawed semen before insemination could increase semen fertility.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2010
E. C. C. Celeghini; Juliana Nascimento; Cláudia Fernandes Raphael; André Furugen Cesar de Andrade; Rubens Paes de Arruda
Neste experimento, foi definida uma combinacao de sondas fluorescentes: iodeto de propidio (PI), aglutinina de Pisum sativum conjugada ao isotiocionato de fluoresceina (FITC-PSA) e JC-1. Para esta proposta, quatro ejaculados de tres carneiros (n=12), que apresentavam motilidade >80% e alteracoes morfologicas <10%, foram diluidos em meio TALP e divididos em duas aliquotas. Uma aliquota foi submetida a tres ciclos de flash frozen e descongelacao, para induzir danos nas membranas celulares e disturbios na funcao mitocondrial. Tres tratamentos foram preparados com as seguintes proporcoes preestabelecidas de semen fresco: semen submetido a flash frozen: 0:100 (T0), 50:50 (T50) e 100:0 (T100). As amostras foram coradas no protocolo proposto e avaliadas por microscopia de epifluorescencia. Para integridade de membrana plasmatica, detectada pela sonda PI, foi obtida a equacao: v=1,09+0,86X (R2=0,98). O acrossomo intacto, verificado pela sonda FITC-PSA, produziu a equacao: v=2,76+0,92X (R2=0,98). O alto potencial de membrana mitocondrial, marcada em vermelho-alaranjado pelo JC-1, foi estimado pela equacao: v=1,90+0,90X (R2=0,98). As equacoes lineares resultantes demonstraram que a tecnica e eficiente e pratica para avaliacao simultânea das membranas plasmatica, acrossomal e mitocondrial em espermatozoides de carneiros.
Revista Brasileira De Zootecnia | 2010
Rubens Paes de Arruda; Daniela Franco da Silva; Maria Augusta Alonso; André Furugen Cesar de Andrade; Juliana Nascimento; Andres Mejia Gallego; Simone Maria Massami Kitamura Martins; Thaís Marques Granato
The industry has made available in the market a series of substances (nutraceuticals) which intent would be to optimize the use of nutrients in some metabolic paths, influencing positively reproductive performance in animals. However, the response to the use of nutraceuticals varies according to the animal. As the organism is highly complex and in order to achieve a perfect activity of the hypothalamic-pituitary-gonadal axis, an ideal interaction in molecular basis is needed, where the nutraceuticals can have their direct action. The aim of this study was to review the function and research results using the main nutraceuticals (β carotene, vitamin A, L-carnitine, omegas 3, 6 and 9 and Gamma-oryzanol) on reproductive characteristics of bulls and stallions.
Brazilian Journal of Poultry Science | 2007
E. C. C. Celeghini; Rubens Paes de Arruda; R Albuquerque; Fha Silva; De Faria; Afc De Andrade; Juliana Nascimento; Cláudia Fernandes Raphael
This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility ≥80% and abnormal morphology ≤10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10 6 spermatozoa/mL) and split into two aliquots, and one of these aliquots was flash frozen in liquid nitrogen and thawed to damage all cellular membranes. Three treatments were prepared from these aliquots, with the following ratios of Fresh semen:Flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen was placed in a microcentrifuge tube with the addition of 2-µL PI, 2-µL MITO, and 50-µL FITC-PSA, and incubated at 38.5 o C/8 min in the dark. An 8µL sample was placed on a slide, coverslipped, and examined by epifluorescence microscopy. Each sample had 200 cells counted and classified based on the fluorescence emitted by each probe. By regression analysis, plasma membrane integrity, as detected by PI, was determined as: v=4.17+0.82X (R 2 =0.95). Acrosome integrity, as detected by FITC-PSA, generated the equation: v=4.19+0.84X (R 2 =0.96). Functional mitochondria was estimated by the equation v=3.20+0.83X (R 2 =0.96). This is an efficient technique to simultaneously evaluate plasmatic, acrosomal, and mitochondrial membranes in fowl sperm. It is suggested that its application in flow cytometry systems allows this methodology to be applied in large scale.
Archive | 2012
Naomi Kondo Nakagawa; Juliana Nascimento; Marina Lazzari Nicola; Paulo Hilário Nascimento Saldiva
An adult man inhales more than 12,000 l of air per day, which may contain particles and microorganisms. The epithelium of the conducting airways, from the nose to the bronchioli, are anatomically and physiologically designed to protect the alveoli by providing clean, warmed and fully saturated air at this level, where an efficient gas exchange must occur, and by providing specific defense mechanisms, such as trapping particles and microorganisms in the mucus and mucociliary transport of these agents in the direction of the oropharynx, where they will be swallowed or expectorated. Mucociliary transport (MCT) is an important respiratory defense mechanism, which efficiency depends on the equilibrium among three major components: ciliary beating, airway surface liquid (the periciliary liquid and mucus), and the interaction between cilia and mucus. In the intensive care unit (ICU) and emergency department, many factors can increase the risks of mucus transport dysfunction. Among them, artificial inspired air conditioning is a basic factor with physiological and clinical impact in critically ill patients.
Animal Reproduction Science | 2008
Eneiva Carla Carvalho Celeghini; Rubens Paes de Arruda; André Furugen Cesar de Andrade; Juliana Nascimento; Cláudia Fernandes Raphael; Paulo Henrique Mazza Rodrigues
Reproduction in Domestic Animals | 2007
E. C. C. Celeghini; R. P. de Arruda; Afc De Andrade; Juliana Nascimento; Cláudia Fernandes Raphael
Reproduction in Domestic Animals | 2007
Afc De Andrade; R. P. de Arruda; E. C. C. Celeghini; Juliana Nascimento; S. M. M. K. Martins; Cláudia Fernandes Raphael; Aníbal de Sant'Anna Moretti
Rev. bras. reprod. anim | 2011
Rubens Paes de Arruda; Daniela Franco da Silva; F. J Affonso; K. M. Lemes; J. D Jaimes; E. C. C. Celeghini; Maria Augusta Alonso; H.F. Carvalho; L. Z Oliveira; Juliana Nascimento