Julie Jepsen Thomas

The Maize TFome – development of a transcription factor open reading frame collection for functional genomics

Establishing the architecture of the gene regulatory networks (GRNs) responsible for controlling the transcription of all genes in an organism is a natural development that follows elucidation of the genome sequence. Reconstruction of the GRN requires the availability of a series of molecular tools and resources that so far have been limited to a few model organisms. One such resource consists of collections of transcription factor (TF) open reading frames (ORFs) cloned into vectors that facilitate easy expression in plants or microorganisms. In this study, we describe the development of a publicly available maize TF ORF collection (TFome) of 2034 clones corresponding to 2017 unique gene models in recombination-ready vectors that make possible the facile mobilization of the TF sequences into a number of different expression vectors. The collection also includes several hundred co-regulators (CoREGs), which we classified into well-defined families, and for which we propose here a standard nomenclature, as we have previously done for TFs. We describe the strategies employed to overcome the limitations associated with cloning ORFs from a genome that remains incompletely annotated, with a partial full-length cDNA set available, and with many TF/CoREG genes lacking experimental support. In many instances this required the combination of genome-wide expression data with gene synthesis approaches. The strategies developed will be valuable for developing similar resources for other agriculturally important plants. Information on all the clones generated is available through the GRASSIUS knowledgebase (http://grassius.org/).

Perceived Risk and its Effects on Quality of Movement in Occupational Performance of Well-Elderly Individuals

The purpose of this study was to determine whether elderly individuals would demonstrate differences in quality of movement when completing a water-pouring task under different levels of perceived risk (i.e., pouring hot water vs. pouring cold water during beverage preparation). Fifty individuals over the age of 65 (M=72.1 years, SD=6.3) participated in the repeated measures, counterbalanced design. Results indicated that participants used less efficient movement dynamics when pouring hot water (higher risk) than when pouring cold water (lower risk). Elders tended to take longer and make more adjustments when pouring hot water than when pouring cold water. Occupational therapists can use this information to better understand the movement dynamics needed during everyday occupations and design therapeutic interventions that appropriately challenge clients.

Rating Scale Analysis and Psychometric Properties of the Caregiver Self-Efficacy Scale for Transfers

ABSTRACT Parents and caregivers faced with the challenges of transferring children with disability are at risk of musculoskeletal injuries and/or emotional stress. The Caregiver Self-Efficacy Scale for Transfers (CSEST) is a 14-item questionnaire that measures self-efficacy for transferring under common conditions. The CSEST yields reliable data and valid inferences; however, its rating scale structure has not been evaluated for utility. The aims of this study were to evaluate the category response structure of the CSEST, test the utility of a revised rating scale structure, and confirm its psychometric properties. The Rasch Measurement Model was used for all analyses. Subjects included 175 adult caregivers recruited from multiple communities. Results confirm that a revised five-category rating scale structure yields reliable data and valid inferences. Given the relationship between self-efficacy and risk of physical and/or emotional stress, measuring parental self-efficacy for transfers is a proactive process in rehabilitation.