Jun-jun Chang

Comparative study of microbial community structure in integrated vertical-flow constructed wetlands for treatment of domestic and nitrified wastewaters

Microbial processes play a vital important role in the removal of contaminants in constructed wetland (CW). However, the microbial physiology and community structure can be influenced by environmental conditions. In this study, four pilot-scale integrated vertical-flow constructed wetlands (IVCWs) were employed to treat domestic and nitrified wastewaters. The microbial properties, along with their response to wastewater quality characteristics and seasonal variation, were determined. The results showed higher Shannon–Weiner diversity (H) and evenness (E) index of fatty acids (FAs), and relative abundances of signature FAs in down-flow cells and in the systems fed with domestic wastewater (DW). The relative abundances of fungi and gram-negative and aerobic bacteria were greater in up-flow cells. The dominant anaerobic bacteria found in most cells might be accounted for the prevailing anaerobic environment within the wetland beds, which could mean that the system fed with nitrified wastewater (NW) should perform better in nitrogen removal. The redundancy analysis (RDA) showed that pollutant concentrations, especially organic matter, influence the FA compositions greatly, and the most significant difference of microbial community structures was detected in down-flow cells fed with DW and up-flow ones with NW. The branched FAs, which could be used to represent anaerobic bacteria, were observed in down-flow cells treating DW and had a significant positive correlation with chemical oxygen demand (COD) concentration, probably suggesting the important role of anaerobic bacteria in organic matter degradation in the IVCWs. Seasonal variation, however, did not greatly influence the microbial community structure in the IVCWs.

Adsorption characteristics of used brick for phosphorus removal from phosphate solution

Phosphorus is one of the main causes for eutrophication in waterbodies. The techniques for phosphorus removal from wastewater and waterbodies have become a hot topic in the world. In this study, used brick was chosen as an experimental adsorbent for removing phosphorus from phosphate solution, and the effects of brick dosage, pH, temperature and vibration time on phosphorus adsorption characteristics were evaluated. Results showed that phosphorus could be effectively removed using brick powders, and the optimum brick dosages were 4, 9 and 35g/L in the presence of 5, 10 and 50mg/L of phosphorus concentrations, respectively. A significant linear correlation (R-2=0.9904) between phosphorus concentration and optimum brick dosage was observed. The optimum condition was determined to be: brick dosage 20g/L, phosphorus concentration 25mg/L, pH 5, temperature 25 degrees C and vibration time 2h.

Effect of intermittent aeration on the treatment performance in a submerged membrane bioreactor

In order to improve removal for nitrogen in a pilot-scale submerged membrane bioreactor (SMBR), intermittent aeration was conducted, and the effect on the treatment performance under four kinds of operation condition (run 1, continuous aeration; run 2, 60/60 min aeration on/off time; run 3, 60/90 min aeration on/off time; run 4, 60/75 min aeration on/off time) was evaluated. The results showed that depending on the specific on/off of the aeration time ratio, removal efficiency of nitrogen could be improved significantly, and the removal rates of total nitrogen (TN) under different operation conditions were 28.0%, 59.5%, 66.8% and 70.7%, respectively. There were no obvious differences for removal rates for CODCr and ammonia among different operation conditions. In general, intermittent aeration could be used as a feasible way to improve treatment performance for nitrogen in the SMBR.

Responses of microbial abundance and enzyme activity in integrated vertical-flow constructed wetlands for domestic and secondary wastewater

Although micro-organisms play a significant role in pollutant removal in constructed wetlands, little is known on the effect of wastewater-quality properties on microbial characteristics. In this study, two groups of integrated vertical-flow constructed wetland microcosms were applied to treat synthetic domestic wastewater and synthetic secondary effluent. The effects of wastewater-quality properties on microbial features were assessed. Results showed that higher values of microbial indicators were observed in the systems with domestic wastewater and in down-flow cells. Redundancy analysis revealed that organic matter concentration and temperature were two critical determinants influencing the microbial features.

Nitrate removal from tail water by integrated vertical-flow constructed wetlands at a high hydraulic loading rate

Nitrate removal rates of two pilot-scale integrated vertical-flow constructed wetlands (IVCWs) treating tail water, under a hydraulic loading rate (HLR) of 250 mm/d with a mean influent NO3--N concentration of 24.4 mg.L-1, were evaluated. Mean NO3--N removal efficiencies of 15.5 and 18.5% with mass removal rates of 1.01 g.m(-2).d(-1) and 1.16 g.m(-2).d(-1) for IVCW 1 (planted with Canna indica and Pontederia cordata) and 2 (planted with Typha orientalis and Arundo donax var. versicolor), respectively, were achieved. The removal rate constants as fitted by the first-order area-based model averaged 0.046 and 0.055 m.d(-1), respectively. Since NO3--N was the dominant nitrogen form in the effluent, denitrification was the limiting step in nitrogen removal despite of favorable pH and anaerobic conditions in the wetland beds. Low availability of carbon source, high HLR, and low temperature could be the probable influencing factors for the observed low NO3--N removal efficiencies. However, IVCW could be used to treat tail water for nitrate removal at a comparable high loading rate.