Jun-ya Okamura

Morphological and optical properties of the corneal lens and retinal structure in the posterior large stemma of the tiger beetle larva

The morphological and optical features of the corneal lens and retina have been examined in the posterior large stemma of the larva of the tiger beetle (Cicindela chinensis). A cup-shaped retina was positioned 55+/-6microm beneath the posterior margin of the corneal lens, which was 479+/-20microm in diameter and 391+/-18microm in thickness (n=41). A light path through an isolated corneal lens showed that the object at infinite distance was focused on the distal margin of the retina. Geometrical optics gave a value of 334+/-15microm (n=55) for the posterior focal length of the corneal lens. The refractive index of the corneal lens was estimated to be around 1.8, if the lens was considered to be homogenous in structure. The internal structure of the lens, including concentric lamellae, was presumed to contribute to such a high refractive index, because this was higher than that of insect cuticle. The retinal structure and how images were blurred at different focus levels were also examined. Data obtained for optics of the corneal lens and retinal structures are discussed with reference to the distinct visual behavior of the larva.

Foraging Navigation of Hornets Studied in Natural Habitats and Laboratory Experiments

Abstract Foraging flights have been studied in three species of hornets (Vespa mandarinia, V. simillima and V. analis) in the field and the laboratory. Hornets seem to use multiple navigational cues for visiting a familiar feeding place. They could orient towards the feeding place immediately after they rose in air from the nest without directly viewing the feeder. They could visit the feeding place after dark at a luminosity 8 lux. These data suggest that they can navigate for some distance with few external cues. Hornets also seem to rely on visual cues for their mid-range navigation. They used some structures on their way as navigational landmarks to negotiate. Individual hornets are supposed to have their own landmarks. Olfactory cues seem to be used to find a new feeding place or to recruit other member. In the approach flight hornets seemed to use multiple visual cues such as the visual characteristics of the feeder and the wider scenery around the feeder. Even if the feeder in training was removed during the test, they flew with a smooth course as if they were pin-pointing the missing feeder, but without sitting on the ground. Hornets learnt how to fly to reach the feeder without external cues after passing by the last visual landmark under conditions with extremely poor visual cues. The present work suggests that hornets retain multiple navigational cues during repeated foraging behavior, and which cues they use seems to depend upon environmental conditions.

Intracellular Responses of Antennal Chordotonal Sensilla of the American Cockroach

The responses of mechanoreceptor neurons in the antennal chordotonal organ have been examined in cockroaches by intracellular recording methods. The chordotonal organ was mechanically stimulated by sinusoidal movement of the flagellum. Stimulus frequencies were varied between 0.5 and 150 Hz. Receptor neurons responded with spike discharges to mechanical stimulation, and were classed into two groups from plots of their average spike frequencies against stimulus frequency. Neurons in one group responded to stimulation over a wide frequency range (from 0.5 to 150 Hz), whereas those in a second group were tuned to higher frequency stimuli. The peak stimulus frequency at which receptor neurons showed maximum responses differed from cell to cell. Some had a peak response at a stimulus frequency given in the present study (from 0.5 to 150 Hz), whereas others were assumed to have peak responses beyond the highest stimulus frequency examined. The timing for the initiation of spikes or of a burst of spikes plotted against each stimulus cycle revealed that spike generation was phase-locked in most cells. Some cells showed phase-independent discharges to stimulation at lower frequency, but increasing stimulus frequencies spike initiation began to assemble at a given phase of the stimulus cycle. The response patterns observed are discussed in relation to the primary process of mechanoreception of the chordotonal organ.

Morphological and physiological identification of medulla interneurons in the visual system of the tiger beetle larva

The morphology of visual interneurons in the tiger beetle larva was identified after recording their responses. Stained neurons were designated as either medulla or protocerebral neurons according to the location of their cell bodies. Medulla neurons were further subdivided into three groups. Afferent medulla neurons extended processes distally in the medulla neuropil and a single axon to the brain through the optic nerve. They received their main input from stemmata on the ipsilateral side. Two distance-sensitive neurons, near-by sensitive and far-sensitive neurons, were also identified. Atypical medulla neurons extended their neurites distally in the medulla and proximally to the brain, as afferent medulla neurons, but their input patterns and the shapes of their spikes differed from afferent neurons. Protocerebral neurons sent a single axon to the medulla neuropil. They spread collateral branches in the posterior region of the protocerebrum on its way to the medulla neuropil. They received main input from stemmata on the contralateral side. Medulla intrinsic neurons did not extend an axon to the brain, and received either bilateral or contralateral stemmata input only. The input patterns and discharge patterns of medulla neurons are discussed with reference to their morphology.

Distance and Size Estimation in the Tiger Beetle Larva: Behavioral, Morphological, and Electrophysiological Approaches

Estimation of the size and distance of an object is one of the important visual functions for a wide variety of animals. How do animals visually measure distance and size? The size of the retinal image of a given object changes as a function of both distance and its absolute size. Since higher animals can learn the absolute size of many objects in their visual worlds, they can estimate the distance to a particular object by its apparent angular size from knowledge of its absolute size gained by memory. Of course, humans can also estimate an object’s distance by binocular disparity.