Jung Oh Ahn
Korea Research Institute of Bioscience and Biotechnology
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Jung Oh Ahn.
BMC Biotechnology | 2012
Yeon-Gu Kim; Byoungwoo Park; Jung Oh Ahn; Joon-Ki Jung; Hong Weon Lee; Eun Gyo Lee
BackgroundThe establishment of high producer is an important issue in Chinese hamster ovary (CHO) cell culture considering increased heterogeneity by the random integration of a transfected foreign gene and the altered position of the integrated gene. Fluorescence-activated cell sorting (FACS)-based cell line development is an efficient strategy for the selection of CHO cells in high therapeutic protein production.ResultsAn internal ribosome entry site (IRES) was introduced for using two green fluorescence protein (GFP) fragments as a reporter to both antibody chains, the heavy chain and the light chain. The cells co-transfected with two GFP fragments showed the emission of green fluorescence by the reconstitution of split GFP. The FACS-sorted pool with GFP expression had a higher specific antibody productivity (qAb) than that of the unsorted pool. The qAb was highly correlated with the fluorescence intensity with a high correlation coefficient, evidenced from the analysis of median GFP and qAb in individual selected clones.ConclusionsThis study proved that the fragment complementation for split GFP could be an efficient indication for antibody production on the basis of high correlation of qAb with reconstitution of GFP. Taken together, we developed an efficient FACS-based screening method for high antibody-producing CHO cells with the benefits of the split GFP system.
Applied Microbiology and Biotechnology | 2004
Jung Oh Ahn; Eui-Sung Choi; Hyeokweon Lee; S. H. Hwang; Chun Suk Kim; Hyeung-Jin Jang; Seungjoo Haam; Joon-Ki Jung
Journal of Microbiology and Biotechnology | 2003
Jung Oh Ahn; Hyung Wook Jang; Hong Weon Lee; Eui Sung Choi; Seung Joo Haam; Tae Kwang Oh; Joon Ki Jung
Biotechnology Letters | 2013
Sasikumar Rajoo; Jung Oh Ahn; Hong Weon Lee; Joon Ki Jung
Archive | 2006
Hong Weon Lee; Jung Oh Ahn; Joon Ki Jung; Eui Sung Choi; Chun Suk Kim; Eun Gyo Lee; Ji Yeon Hong; Hyeok Won Lee; Myoung Soo Park
Process Biochemistry | 2011
Yeon-Gu Kim; Jee Yon Kim; Byoungwoo Park; Jung Oh Ahn; Joon-Ki Jung; Hong Weon Lee; Gyun Min Lee; Eun Gyo Lee
Archive | 2006
Hong Weon Lee; Jung Oh Ahn; Joon Ki Jung; Eui Sung Choi; Chun Suk Kim; Eun Gyo Lee; Ji Yeon Hong; Hyeok Won Lee; Myoung Soo Park
Archive | 2014
Eui Sung Choi; 최의성; Kyung Min Park; 박경민; Sung Hwa Seo; 서성화; Jung Oh Ahn; 안정오; Eun Gyo Lee; 이은교; Chun Sug Kim; 김천석; In Joong Yoon; 윤인중; Sung Sik Yoo; 유성식; Young Jung Shim; 심영정
Archive | 2014
Eui Sung Choi; 최의성; Kyung Min Park; 박경민; Sung Hwa Seo; 서성화; Jung Oh Ahn; 안정오; Eun Gyo Lee; 이은교; Chun Sug Kim; 김천석; In Joong Yoon; 윤인중; Sung Sik Yoo; 유성식; Young Jung Shim; 심영정
Process Biochemistry | 2012
Yeon-Gu Kim; Byoungwoo Park; Seungwoon Lee; Jung Oh Ahn; Joon-Ki Jung; Hong Weon Lee; Gyun Min Lee; Eun Gyo Lee
Collaboration
Dive into the Jung Oh Ahn's collaboration.
Korea Research Institute of Bioscience and Biotechnology
View shared research outputs