Jung-Youn Park

Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene

Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis′ economic value, however, is low in China, and much of the catch in China is exported to Korea at a low price, thus leading to potential false labeling. For this reason, we developed a simple method to identify A. chinensis′ origin using allele-specific polymerase chain reaction (PCR). Ten single nucleotide polymorphisms (SNPs) were identified from partial (i.e., 570 bp) DNA sequence analysis of the mitochondrial 16s rRNA gene in 96 Korean and 96 Chinese individual shrimp. Among 10 SNP sites, four sites were observed in populations from both countries, and two sites located in the middle with SNP sites at their 3′-ends were used to design allele-specific primers. Among the eight internal primers, the C220F primer specific to the Chinese A. chinensis population amplified a DNA fragment of 364 bp only from that population. We were able to identify the A. chinensis population origin with 100% accuracy using multiplex PCR performed with two external primers and C220F primers. These results show that the 16S rRNA gene that is generally used for the identification of species can be used for the identification of the origin within species of A. chinensis, which is an important finding for the fair trade of the species between Korea and China.

Comparison of Fish Species Composition Collected by Set Net at Hupo in Gyeong-Sang-Buk-Do, and Jangho in Gang-Won-Do, Korea

우리나라 동해는 해안선이 단조롭고, 섬과 만이 거의 없으며, 해류 역시 매우 단조로운 구조를 이룬다. 동해는 수심 300-400 m의 표층수와 그 이심의 동해고유수로 구분되며, 동해의 해황 은 이들 두 수괴의 상호작용으로 변동한다. 또한, 동해에는 북 상하는 대마난류와 남하하는 북한한류가 북위 38-41° 부근에 서 충돌하여 동서방향으로 아극전선(subpolar front)이 형성된 다(Isoda, 1991; Cho et al., 2004). 그외 동해 남부의 대마난류 수괴를 중심으로 주변의 수괴들이 만나 형성되는 상대적으로 불안정한 열전선이 존재한다(Choi et al., 2010). 동해에 형성 되는 것으로 알려진 다양한 전선은 동중국해에서 산란된 고등 어, 전갱이, 꽁치 등 회유성 어종의 유어가 동해로 수송되는 도 중에 풍부한 먹이를 제공해 준다(Cho et al., 2004). 이처럼 전선 역은 풍부한 생산력으로 다양한 해양생물이 서식하기에 적합 하며(Browan and Easias, 1977; Gong and Son, 1982), 전선역 이 생물에 미치는 영향에 대해 동물플랑크톤(Park et al., 1998; Rebstock and Kang, 2003), 오징어(Cho et al., 2004), 멸치(Lee and Kim, 2007) 등을 대상으로 한 연구가 있으나 어류 분포에 관한 연구는 없다. 경상북도 후포와 강원도 장호에서 정치망으로 채집된 어류 종조성 비교

Genetic Differences between Wild and Cultured Populations in Olive Flounder in Korea Based on Mitochondrial DNA Analysis

We sequenced a 522 bp fragment including the tRNA Thr , tRNA Pro gene and the first half of the control region from 29 wild and cultured olive flounder specimens from Korea. Out of 522 nucleotide sites, 49 (9.4%) were variable, 23 haplotypes being found. Most haplotypes are unique in the wild population and only four were shared by cultured specimins. The nucleotide diversity and differences between wild and cultured populations were 0.025±0.013 and 0.015±0.008, and 12.94±6.00 and 7.83±3.75, respectively. Haplotype diversity was 0.98±0.02 and 0.49±0.09 in the wild and cultured populations, respectively. These results show that marked reductions of genetic variability in the hatchery strains were observed in the number of mitochondrial DNA haplotypes and haplotype diversity when compared to the wild populations. Furthermore, we detected significant population differentiation between both populations. The mtDNA sequencing technique used to evaluate the genetic variability of hatchery strains compared to that of the wild population is potential for genetic monitoring of olive flounder hatchery stocks.

Yearly Fluctuation in the Fish Species Composition of Shrimp Beam Trawls off Maemuldo, Korea, during 2007-2009

We investigated yearly fluctuation of the fish species composition of beam trawls off Maemuldo in the east southern sea of Korea, from March 2007 to November 2009. A total of 75 fish species were collected during the period. The number of fish species accumulated amounted to 54, 64 and 75 species in 2007, 2008 and 2009, respectively. The number of newly occurring species increased with time. The number of expected resident species in Maemuldo was estimated as 9 species including Conger myrister, Okamejei kenojei and Pholis nebulosa, each of which appeared more than 14 out of a total 27 times. Cluster analysis showed that the years 2007 and 2008 were closely clustered, while the year 2009 was distantly clustered with 2007 and 2008. This may be due to the high catch ratio of Clidoderma asperrimum in 2009 alone, when a low water temperature phenomenon was observed unlike the situation in 2007 and 2008.

A New Record of a Spot-fin Porcupinefish, Diodon hystrix (Diodontidae, Pisces) off Jeju Island, Korea

A single specimen belonging to the family Diodontidae was collected from Seo-gwi-po, Jeju Island, Korea in July 2013 and was identified as Diodon hystrix Linnaeus, 1758, a species new to the Korean fish fauna. The species is characterized by 14 dorsal fin rays, 14 anal fin rays, spines on the dorsal and ventral surfaces of the caudal peduncle, and many small black spots on all fins except the anal fin. The most similar species, Diodon holocanthus, is well distinguished from D. hystrix by the absence of black spots on the dorsal and caudal fins, and the lack of a spine on the dorsal surface of the caudal peduncle. Accordingly, we describe herein the morphological traits of D. hystrix and suggest its new Korean name, “Jan-jeom-bak-i-ga-si-bok”.