Junichi Odo

Determination of hydrogen peroxide with N,N-diethylaniline and 4-aminoantipyrine by use of an anion-exchange resin modified with manganese-tetrakis(sulphophenyl)porphine, as a substitute for peroxidase

Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H(2)O(2) + N,N-diethylaniline + 4-aminoantipyrine (catalyst)--> quinonoid dye (lambda(max) 550 nm) + 4H(2)O. The apparent molar absorptivity for hydrogen peroxide was 1.1 x 10(4) 1.mole(-1).cm(-1), coefficient of variation 0.7%. This value is approximately 84% of that obtained by the use of peroxidase as catalyst. Similar conditions to those in the enzymatic reaction were suitable for use of the modified resin as catalyst, and the results show it to be a good substitute for peroxidase in this reaction system.

Determination of hydrogen peroxide by use of an anion-exchange resin modified with manganese tetrakis (sulfophenyl) porphine as a mimesis of peroxidase

Abstract The peroxidase-like activity of the modified resin is demonstrated in its use for the determination of hydrogen peroxide by means of the chromogenic reaction with 4-aminoantipyrine and phenol. The sensitivity achieved is 80% of that obtained with peroxidase; the r.s.d, is 1.1%, and the catalyst can be reused without deterioration for up to 10 assays.

Uricase-like catalytic activity of ion-exchange resins modified with metalloporphyrins.

The uricase-like catalytic activity of the ion-exchange resins modified with metalloporphyrins has been investigated through the oxidation of uric acid. The anion-exchange resins modified with Mn(3+)-tetrakis(sulfophenyl)porphine and the cation-exchange resin modified with Mn(3+)-tetrakis(1-methylpyridinium-4-yl)porphine exhibited the highest uricase-like activity among the modified resins tested. The fact that these resins accelerated the oxidation of uric acid even after ten cycles of use indicates that the modified resins act as catalysts in the reaction catalysed by uricase. Some of the modified resins may be effectively used for the determination of uric acid in place of uricase.

Flow Analysis for Determination of Hydrogen Peroxide in Environmental Water by the Use of Biomimetric Functional Resin

We have developed a novel method of flow analysis for hydrogen peroxide, which is applicable to environmental water samples, including rain. The present method is based on the measurement of the absorbance of dye formed by the following reaction using an anion-exchange resin functionalized with manganese (M)- tetrakis(sulfophenyl)porphine (Mn-TPPS r )as a catalyst instead of horseradish peroxidase.