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Dive into the research topics where Junichi Tanishita is active.

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Featured researches published by Junichi Tanishita.


Journal of Fermentation and Bioengineering | 1989

Identification of an ice-nucleating bacterium and its ice nucleation properties

Hitoshi Obata; Tsuyoshi Nakai; Junichi Tanishita; Tai Tokuyama

Abstract An ice-nucleating bacterium, KUIN-2, was isolated from carrot leaves. The ice-nucleating bacterium was found in the white colony group. KUIN-2 was identified as Pseudomonas viridiflava from its taxonomic characteristics. When KUIN-2 was cultured aerobically in a medium consisting of Trypticase soy broth (pH 6.0) for 24 h at 18°C, the ice-nucleating activity of KUIN-2 cells was obtained. Ice nucleation was detected at −2.8°C in cell suspensions (1.4 × 10 8 cells/ml) of KUIN-2. The nucleation frequency of KUIN-2 was greatly inhibited by the addition of urea or N -ethylmaleimide.


Journal of Fermentation Technology | 1987

Ice-nucleating activity of Pseudomonas fluorescens

Hitoshi Obata; Youji Saeki; Junichi Tanishita; Tai Tokuyama

Abstract Pseudomonas fluorescens KUIN-1 can cause the freezing of water at relatively warm termperatures (−2.9 to −30°C). Glucose, glycerol, and citric acid were good substrates yielding cells with high ice-nucleating activity. Ammonium salts were good nitrogen sourcfes yielding cells with high ice-nucleating activity. The ice-nucleating activity of KUIN-1 was not affected by pH between pH 5.5 to pH 8.0. The ice-nucleating activity of cells was greatly influenced by heat treatment.


Agricultural and biological chemistry | 1990

Identification of a New Ice-nucleating Bacterium and Its Ice Nucleation Properties

Hitoshi Obata; Kouji Takinami; Junichi Tanishita; Yoshie Hasegawa; Shohei Kawate; Tai Tokuyama; Teruo Ueno


Agricultural and biological chemistry | 1987

Identification of an Ice-nucleating Bacterium KUIN-1 as Pseudomonas fluorescens and Its Ice Nucleation Properties

Hitoshi Obata; Youji Saeki; Junichi Tanishita; Tai Tokuyama; Hideaki Hori; Yoshikage Higashi


Journal of Food Science and Technology-mysore | 1986

Immobilization of Catalase on Porous Acid Clay

Junichi Tanishita; Hitoshi Obata; Tai Tokuyama


Journal of Food Science and Technology-mysore | 1987

Determination of Ascorbic Acid Kineticaly by Using Ascorbate Oxidase

Hitoshi Obata; Yasuhito Taga; Junichi Tanishita; Tai Tokuyama


Bulletin of the Agricultural Chemical Society of Japan | 1987

Ice-nucleating microorganisms. Part II. Effect of carbon sources on the ice-nucleating activity of Pseudomonas fluorescens KUIN-1.

Hitoshi Obata; Kiyonori Dobashi; Junichi Tanishita; Tai Tokuyama


Bulletin of the Agricultural Chemical Society of Japan | 1987

Oxidation of reductones with ascorbate oxidase.

Hitoshi Obata; 康仁 多賀; Junichi Tanishita; Tai Tokuyama


Agricultural and biological chemistry | 1985

Measurement of Reaction Rate of 3-Hydroxy-2-pyrone with 2, 6-Dichlorophenolindophenol

Hitoshi Obata; Nobuki Fujiwara; Junichi Tanishita; Tai Tokuyama


Agricultural and biological chemistry | 1984

Studies on the Reduction of 2,6-Dichlorophenolindophenol by 6-Substituted 3-Hydroxy-4-Pyrones, Using a Stopped-flow Method

Hitoshi Obata; Nobuki Fujiwara; Junichi Tanishita; Tai Tokuyama

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Hideaki Hori

Osaka Dental University

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