Junke Fu
Xi'an Jiaotong University
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Featured researches published by Junke Fu.
Tumor Biology | 2015
Jingyao Zhang; Qifei Wu; Zhe Wang; Yong Zhang; Guang-Jian Zhang; Junke Fu; Chang Liu
Partner of sld five 1 (PSF1) is a member of the heterotetrameric complex termed GINS. Previous studies have shown that PSF1 is unregulated in several cancer and associated with tumor malignant characters. However, the effects of PSF1 in lung cancer are still unclear. The goal of this study was to investigate the effects of PSF1 on the proliferation capacities of lung cancer. To start with, expression of PSF1 in 22 human lung cancer samples and adjacent non-tumor samples were detected by real-time RT-PCR and Western blotting. Our results showed that PSF1 was overexpressed in lung cancer samples compared to adjacent non-tumor samples. To achieve better insights of PSF1 functions in lung cancer cells, we used PSF1-specific small interfering RNA (siRNA) successfully inhibit the expression of PSF1 in messenger RNA (mRNA) and protein levels. In addition, we used lung cancer cell lines with different p53 gene background (p53 null and p53 wild-type). The results showed that knockdown of PSF1 inhibited cell proliferation and caused cell cycle arrest of lung cancer cells in a p53-independent manner. Our data indicated that PSF1 is functionally involved in lung cancer cell proliferation and is a potential target for lung cancer therapy.
Medical Principles and Practice | 2012
Guang-Jian Zhang; Rui Gao; Junke Fu; Xin Jin; Yong Zhang; Zhe Wang
Objective: To study the correlation between climatic changes and the development of primary spontaneous pneumothorax (PSP). Subjects and Methods: We retrospectively studied the relationship between 337 patients with conservatively treated PSP and meteorological conditions during a 3-year period in the urban area of Xi’an, China. The comparison was made depending on solar terms and on different aspects of atmospheric pressure, outdoor temperature, relative humidity, and wind speed. Results: Significant differences were found between PSP and non-PSP days depending on daily mean values of outdoor temperature and atmospheric pressure (p = 0.001 and p < 0.001, respectively). However, no obvious differences of meteorological factor variations between the ‘PSP day’ and the ‘pre-PSP day’ on days with and without PSP were found. The occurrence of PSP was associated with the solar terms Spring Equinox (p < 0.05) and End of Heat (p < 0.01). Conclusion: Among the factors examined in our study, daily mean outdoor temperature and atmospheric pressure showed a strong correlation with the occurrence of PSP. The solar terms Spring Equinox and End of Heat were found to be closely related with PSP development, which shed light on a new way for PSP incidence evaluation.
Asian Pacific Journal of Cancer Prevention | 2013
Xin-Ju Li; Qi-Fei Wu; Da-Lin He; Junke Fu; Xin Jin
OBJECTIVES This study employed proteomic profiling to identify specific tumor markers that might improve early diagnosis of lung squamous cell carcinoma. METHODS Serum samples were isolated from 30 patients with stage I lung squamous cell carcinoma and 30 age-and gender-matched healthy controls, and proteomic profiles were obtained by matrix-assisted laser desorption ionization time of flight mass spectrometry. RESULTS Three highly expressed potential tumor markers were identified in the sera of stage I lung squamous cell carcinoma patients, with molecular weights of 3261.69, 3192.07, and 2556.92 Da. One protein peak with molecular weight 3261.69 Da was chosen as the candidate biomarker and identified as a fibrinogen alpha chain through a search of the IPI, NCBI or SWISS-PROT protein databases. CONCLUSION As a potential tumor biomarker, fibrinogen alpha chain may be applicable for the early diagnosis and prognosis of lung squamous cell carcinoma patients.
Thoracic Cancer | 2011
Zhe Wang; Junke Fu; Dongmei Diao; Chengxue Dang
Background: There has been little research on perioperative hypercoagulability in non‐small‐cell lung cancer patients. The D‐dimer can be used to evaluate the hypercoagulability in cancer patients.
Oncotarget | 2016
Nanzheng Chen; Xiaomei Yang; Wen Guo; Jiangtao You; Qifei Wu; Guang-Jian Zhang; Haijun Li; Donghong Geng; Tianbo Jin; Junke Fu; Yong Zhang
Single nucleotide polymorphisms (SNPs) in the telomere-associated gene ACYP2 are associated with increased lung cancer risk. We explored the correlation between ACYP2 SNPs and lung cancer susceptibility in the Chinese Han population. A total of 554 lung cancer patients and 603 healthy controls were included in this study. Thirteen SNPs in ACYP2 were selected. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated using unconditional logistic regression analysis. Multivariate logistic regression analysis was used assess the correlation between SNPs and lung cancer. We found that rs1682111 was associated with increased lung cancer risk in the recessive model (crude, OR=1.50, 95%CI: 1.04-2.16, p=0.029; adjusted for age, OR=1.55, 95%CI: 1.04-2.30, p=0.029), as was rs11896604 in the codominant model (crude, OR=0.65, 95%CI: 0.33-1.28, p=0.045; adjusted for age, OR=0.74, 95%CI: 0.36-1.53, p=0.049) and over-dominant model (crude, OR=1.30, 95%CI: 1.02-1.66, p=0.032; adjusted for age, OR=1.37, 95%CI: 1.05-1.78, p=0.020). Finally, rs843720 was associated with increased lung cancer risk in the recessive model (crude, OR=1.43, 95%CI: 1.02-2.02, p=0.040; adjusted for age, OR=1.48, 95%CI: 1.02-2.15, p=0.040). Thus three SNPs in ACYP2 (rs1682111, rs11896604 and rs843720) associate with lung cancer in the Chinese Han population.
Chinese journal of lung cancer | 2011
Zhe Wang; Junke Fu; Dongmei Diao; Chengxue Dang
BACKGROUND AND OBJECTIVE Some operable non-small cell lung cancer (NSCLC) patients have poor prognosis shortly after the surgery. D-dimer (DD) is an independent prognosis factor of lung cancer, especially for inoperable patients. The aim of the study is to investigate whether the pre-operative plasma DD level could predict the poor prognosis shortly after the surgery in operable NSCLC patients. METHODS The pre-operative plasma DD level of 56 newly diagnosed NSCLC patients without metastasis was examined. All the patients had been followed for one year post-operatively and the end-point was the occurrence of the poor prognosis incident including any sign of the metastasis, local recurrence or death related with the lung cancer. Difference of prognosis according to pre-operative plasma DD level was compared by Chi-square test. Diseases progress was analyzed by Kaplan-Meier method. RESULTS Among 56 NSCLC patients, 91% had received the curative resections (44 lobectomy and 7 pneumonectomy). There were still 2 cases of the wedge resection and 3 cases of the exploration. The median of the pre-operative plasma DD level was 1.05 (0.55) mg/L. The patients were allocated into two subgroups by the median of the DD levels. There were 11 patients with poor prognosis within one year after the resection in the high DD subgroup, while 3 patients in the low DD subgroup (P=0.03, OR=4.89, 95%CI: 1.2-20.1). The diseases progress curves were significantly different between the high and low subgroups (P=0.024). Based on plasma DD level, the poor prognosis incident within one year after the surgery was best predicted in the early stage (I, II) of the NSCLC, especially in adenocarcinoma patients. CONCLUSION The pre-operative plasma DD levels may predict the poor prognosis within one year after the surgery in NSCLC. The measurement of the fibrinolysis marker may help to exclude the unfit patients for the surgery.
Thoracic Cancer | 2015
Yong Zhang; Chunbao Wang; Guang-Jian Zhang; Zhe Wang; Xiaopeng Wen; Xiaomei Yang; Jiangtao You; Junke Fu
Polymorphous low‐grade adenocarcinoma (PLGA) is a low‐grade malignant infiltrative tumor of the minor salivary glands. According to data from PubMed (National Center for Biotechnology), one case of PLGA involving the left main bronchus and one case involving the right main and upper lobar bronchi have previously been reported. Here, we describe the first case of PLGA originating in the trachea with metastasis to the right middle lobe bronchus, all initially misdiagnosed as adenoid cystic carcinoma (ACC). It is particularly important to distinguish this tumor from other types of salivary gland tumors, especially ACC. Complete surgical excision is the curative treatment of choice for PLGA.
Thoracic Cancer | 2013
Junke Fu; Qifei Wu; Yong Zhang; Xufeng Zhang; Zhe Wang
We report a case of primary inflammatory myofibroblastic tumor of the diaphragm in a 64‐year‐old man. The patient was hospitalized for a computed tomography (CT)‐detected large tissue mass at the left lower lung field. Complete tumor excision followed by pathological investigation was performed. Microscopically, the tumor showed staggered arrangements of spindle myoepithelial cells, lymphoblastic and eosinophil cells. Immunohistochemically, the proliferating spindle cells showed positive staining for smooth muscle actin, vimentin, CD68 and Desmin, but negative for cytokeratin, leukocyte common antigen, epithelial membrane antigen, and S‐100. This is the first reported inflammatory myofibroblastic tumor of the diaphragm found in an adult. The postoperative course was uneventful and the patient had no recurrence 18 months after surgery.
Video-Assisted Thoracic Surgery | 2017
Yong Zhang; Shuo Li; Haiqi He; Lei Ma; Xiaomei Yang; Donghong Geng; Jiangtao You; Junke Fu
Background: Minimally invasive esophagectomy has been developed to reduce surgical injury. In this current study, we have designed a new operative procedure to dissect the esophagus with less chest injury. Methods: Three pigs (Sus domesticus) underwent the same procedures: routine laparoscopic techniques, anastomosis at left neck and right thoracoscopic everted esophagectomy utilizing one thoracoscopic operative hole. Results: The everted resection of the esophagus was successfully accomplished in the three pigs utilizing one thoracoscopic operative hole. Conclusions: A novel technique was described here. The procedure of the operation was modified with anastomosis operated before esophagectomy. Only one thoracic operative hole was needed, which reduced the injury on chest wall.
Journal of Surgical Research | 2015
Qifei Wu; Jingyao Zhang; Yong Wan; Si-Dong Song; Yong Zhang; Guang-Jian Zhang; Chang Liu; Junke Fu
BACKGROUND With the development of thoracic surgeries, one-lung ventilation (OLV) has been routinely used to facilitate surgical exposure. However, OLV can cause lung injury during the surgical process and becomes an important factor affecting the outcomes. To date, effective treatments for the prevention of lung injury caused by OLV are lacking. Hydrogen has been demonstrated to have effective protection against tissue injuries caused by oxidative stress, inflammation, and apoptosis. This study investigated the efficacy of hydrogen water consumption on the prevention of lung injury induced by OLV in rats. MATERIALS AND METHODS Male Sprague-Dawley rats (n = 32, 240-260 g) were divided randomly into the following four groups: sham group, sham + H2 group, OLV group, OLV + H2 group. The rats drank hydrogen water or degassed hydrogen water for 4 wk before the operation and received OLV for 60 min and two-lung ventilation for 60 min. Lung tissues were assayed for wet-to-dry ratio, oxidative stress variables, proinflammatory cytokines, and hematoxylin-eosin staining. RESULTS Hydrogen water consumption reduced wet-to-dry weight ratio, malondialdehyde and myeloperoxidase activity and decreased the concentration of TNF-α, IL-1β, and IL-6 in the lung tissues compared with sham group and sham + H2 group. Hydrogen water consumption further attenuated NF-κB activation and caused histopathologic alterations. CONCLUSIONS Our data demonstrated that hydrogen water consumption ameliorated OLV-induced lung injury, and it may exert its protective role by its anti-inflammation, antioxidation and reducing NF-κB activity in the lung tissues.