Jürgen Hess
University of Ulm
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Featured researches published by Jürgen Hess.
Molecular Genetics and Genomics | 1988
Monika Vogel; Jürgen Hess; Irene Then; Antonio Juárez; Werner Goebel
SummaryA sequence (hlyR) of about 600 bp which enhances the expression of hemolysin (HlyA) more than 50-fold was identified in the plasmid pHly152-specific hemolysin (hly) determinant. Deletion of this entire hlyR sequence led to the same low level of hemolysin synthesis and excretion as that expressed by the recombinant plasmid pANN202-312. HlyR was active in cis but its activity was orientation-dependent. The enhancing sequence, hlyR, is separated from the promoter phlyI transcribing hlyC, hlyA and possibly hlyB by more than 1.5 kb including an IS2 element. Stepwise removal of the hlyR sequence from its 5′ end by exonuclease III (ExoIII) digestion yielded several types of deletion mutants which expressed decreasing amounts of hemolysin. A similar observation was made when hlyR was shortened by ExoIII from its 3′ end, which suggests that more than one functional region may be present in the hlyR sequence. A deletion of 717 bp within the adjacent IS2 element reduced the activity of hlyR only slightly, indicating that IS2 is not directly involved in the enhancement mechanism but that it may support an optimal positioning in hlyR relative to the hly promoter. The nucleotide sequence of hlyR is rich in A+T and does not contain an extended open reading frame, but exhibits several sequence motives that may represent sites for protein binding and DNA bending.
Vaccine | 2003
Guido Dietrich; Jean-François Viret; Jürgen Hess
Mycobacterium bovis Bacille Calmette-Guérin (BCG) is one of the most widely used vaccines. Modern techniques in genome manipulation allow the construction of recombinant (r)-BCG strains that can be employed as highly immunogenic vaccines against tuberculosis (TB) with an enhanced safety profile. In addition, the development of novel procedures to cultivate BCG will allow the large-scale production of future BCG-based vaccines.
Zentralblatt Fur Bakteriologie-international Journal of Medical Microbiology Virology Parasitology and Infectious Diseases | 1996
Jürgen Hess; Anja Dreher; Ivo Gentschev; Werner Goebel; Christoph H. Ladel; Diana Miko; Stefan H. E. Kaufmann
The role of p60 in intestinal invasion by Listeria monocytogenes was assessed after oral infection of mice with the p60 low-expressing mutant RIII, or with anti-p60 antibody coated wild-type EGD. Invasion by L. monocytogenes RIII bacteria has been unimpaired suggesting that a low density of p60 suffices for entry. Up to 24 h post infection (p.i.), intestinal penetration by L. monocytogenes EGD bacteria was markedly reduced by coating with anti-p60 antibodies. In histological sections, anti-p60 antibody-treated L. monocytogenes EGD, but not uncoated listeriae were still detectable 24 h p.i. at the apical surface of enterocytes in the intestine. We conclude that p60 contributes to host invasion through the natural port of listerial entry, the intestinal epithelium.
International Journal of Medical Microbiology | 2003
Guido Dietrich; Jean-François Viret; Jürgen Hess
In this manuscript, we will review the utilization of Mycobacterium bovis Bacille Calmette-Guerin (BCG) as a vaccine against tuberculosis (TB) and as a carrier system for heterologous antigens. BCG is one of the most widely used vaccines. Novel techniques in genome manipulation allow the construction of virulence-attenuated recombinant (r)-BCG strains that can be employed as homologous vaccines, or as heterologous antigen delivery systems, for priming pathogen-specific immunity against infectious diseases, including TB. Several approaches are available for heterologous antigen expression and compartmentalization in BCG and recent findings show the potential to modulate and direct the immune responses induced by r-BCG strains as desired. Recent achievements in complete genome analysis of various target pathogens, combined with a better understanding of protective pathogen-specific immune responses, form the basis for the rational design of a new generation of recombinant mycobacterial vaccines against a multitude of infectious diseases.
International Immunology | 1994
Inge E. A. Flesch; Jürgen Hess; I P Oswald; Stefan H. E. Kaufmann
European Journal of Immunology | 1995
Christoph H. Ladel; Jürgen Hess; Sabine Daugelat; Peter Mombaerts; Susumu Tonegawa; Stefan H. E. Kaufmann
Infection and Immunity | 1995
Jürgen Hess; Ivo Gentschev; Gudrun Szalay; Christoph H. Ladel; Andreas Bubert; Werner Goebel; Stefan H. E. Kaufmann
Gene | 1996
Ivaylo Gentschev; Hans J. Mollenkopf; Zeljka Sokolovic; Jürgen Hess; Stefan H. E. Kaufmann; Werner Goebel
European Journal of Immunology | 1994
Gudrun Szalay; Jürgen Hess; Stefan H. E. Kaufmann
Infection and Immunity | 1995
Gudrun Szalay; Jürgen Hess; Stefan H. E. Kaufmann