Jürgen Van Erps

Exploration and classification of chromatographic fingerprints as additional tool for identification and quality control of several Artemisia species

The World Health Organization accepts chromatographic fingerprints as a tool for identification and quality control of herbal medicines. This is the first study in which the distinction, identification and quality control of four different Artemisia species, i.e. Artemisia vulgaris, A. absinthium, A. annua and A. capillaris samples, is performed based on the evaluation of entire chromatographic fingerprint profiles developed with identical experimental conditions. High-Performance Liquid Chromatography (HPLC) with Diode Array Detection (DAD) was used to develop the fingerprints. Application of factorial designs leads to methanol/water (80:20 (v/v)) as the best extraction solvent for the pulverised plant material and to a shaking bath for 30 min as extraction method. Further, so-called screening, optimisation and fine-tuning phases were performed during fingerprint development. Most information about the different Artemisia species, i.e. the highest number of separated peaks in the fingerprint, was acquired on four coupled Chromolith columns (100 mm × 4.6 mm I.D.). Trifluoroacetic acid 0.05% (v/v) was used as mobile-phase additive in a stepwise linear methanol/water gradient, i.e. 5, 34, 41, 72 and 95% (v/v) methanol at 0, 9, 30, 44 and 51 min, where the last mobile phase composition was kept isocratic till 60 min. One detection wavelength was selected to perform data analysis. The lowest similarity between the fingerprints of the four species was present at 214 nm. The HPLC/DAD method was applied on 199 herbal samples of the four Artemisia species, resulting in 357 fingerprints. The within- and between-day variation of the entire method, as well as the quality control fingerprints obtained during routine analysis, were found acceptable. The distinction of these Artemisia species was evaluated based on the entire chromatographic profiles, developed by a shared method, and visualised in score plots by means of the Principal Component Analysis (PCA) exploratory data-analysis technique. Samples of different quality could be indicated on the score plots. No multi-component analysis was required to reach the goal. Furthermore, differences related to the origin of some of the not-certified samples were shown. The importance of the specific herbal part used for its identification was also presented. In addition, no differences were observed among fingerprints of lyophilised or conditioned-air dried samples. Finally, a classification technique, Soft Independent Modelling by Class Analogy (SIMCA), was successfully evaluated as identification technique for unknown samples. Six additional Artemisia species (29 herbal samples) were identified as not belonging to any of the four modelled classes. The developed chromatographic fingerprints and the evaluation of the entire profiles provide an added value to the distinction, identification and quality control of the simultaneously investigated Artemisia species.

100 nm period grating by high-index phase-mask immersion lithography

The interferogram of a high index phase mask of 200 nm period under normal incidence of a collimated beam at 244 nm wavelength with substantially suppressed zeroth order produces a 100 nm period grating in a resist film under immersion. The paper describes the phase mask design, its fabrication, the effect of electron-beam lithographic stitching errors and optical assessment of the fabricated sub-cutoff grating.

Demonstration of a multichannel, multiresolution imaging system

In conventional multichannel imaging systems, all channels have similar imaging properties [field-of-view (FOV) and angular resolution]. In our approach, channels are designed to have different imaging properties which add multiresolution capability to the system. We have experimentally demonstrated, for the first time to our knowledge, a three-channel imaging system which simultaneously captures multiple images having different magnifications and FOVs on an image sensor. Each channel consists of four aspherical lens surfaces fabricated from four PMMA plates by ultraprecision diamond tooling and of a baffle made from a titanium (Ti) and aluminum (Al) based metal alloy. The integrated imaging system is able to record a FOV of 7.6° with the first channel and 73° with the third channel while having a magnification ratio of about 6 between them. The experimental and simulation results, specifically the FOV and magnification ratios, are comparable, and this paves a way for low-cost, compact imaging systems which can embed smart imaging functionalities.

Automatic dispersion compensation for 1.28Tb/s OTDM signal transmission using photonic-chip-based dispersion monitoring

We present automatic dispersion control of 1.28Tb/s optical time domain multiplexed signals. The dispersion is monitored by measuring the power of the 1.28THz tone of the RF spectrum using a photonic-chip-based radio-frequency spectrum analyzer (PC-RFSA) and the dispersion compensation is realized by means of a spectral pulse shaper, via computer-controlled feedback from the PC-RFSA.

Design and tolerance analysis of a low bending loss hole-assisted fiber using statistical design methodology

We present the design of a low bending loss hole-assisted fiber for a 180?-bend fiber socket application, including a tolerance analysis for manufacturability. To this aim, we make use of statistical design methodology, combined with a fully vectorial mode solver. Two resulting designs are presented and their performance in terms of bending loss, coupling loss to Corning SMF-28 standard telecom fiber, and cut-off wavelength is calculated.

Enhanced cross phase modulation instability in birefringent photonic crystal fibers in the anomalous dispersion regime

We study Cross Phase Modulational Instability (CPMI) -a particular form of vector modulational instability- in the anomalous dispersion regime in highly birefringent, strongly dispersive optical fibers. When the pump power is high, the detuning of the Scalar Modulational Instability (SMI) is comparable to the detuning of the CPMI. The gain of the CPMI -which is usually much smaller than the gain of the SMI-, is then strongly enhanced and becomes much larger than the gain of the SMI. This theoretical prediction is well verified experimentally using small core photonic crystal fibers.

Laser ablation- and plasma etching-based patterning of graphene on silicon-on-insulator waveguides.

We present a new approach to remove monolayer graphene transferred on top of a silicon-on-insulator (SOI) photonic integrated chip. Femtosecond laser ablation is used for the first time to remove graphene from SOI waveguides, whereas oxygen plasma etching through a metal mask is employed to peel off graphene from the grating couplers attached to the waveguides. We show by means of Raman spectroscopy and atomic force microscopy that the removal of graphene is successful with minimal damage to the underlying SOI waveguides. Finally, we employ both removal techniques to measure the contribution of graphene to the loss of grating-coupled graphene-covered SOI waveguides using the cut-back method.

B-CALM: AN OPEN-SOURCE MULTI-GPU-BASED 3D-FDTD WITH MULTI-POLE DISPERSION FOR PLASMONICS

Numerical calculations based on finite-difference timedomain (FDTD) simulations for metallic nanostructures in a broad optical spectrum require an accurate modeling of the permittivity of dispersive materials. In this paper, we present the algorithms behind B-CALM (Belgium-CAlifornia Light Machine), an open-source 3D-FDTD solver simultaneously operating on multiple Graphical Processing Units (GPUs) and efficiently utilizing multi-pole dispersion models while hiding latency in inter-GPU memory transfers. Our architecture shows a reduction in computing times for multi-pole dispersion models and an almost linear speed-up with respect to the amount of used GPUs. We benchmark B-CALM by computing the absorption efficiency of a metallic nanosphere in a broad spectral range with a six-pole Lorentz model and compare it with Mie theory and with a widely used Central Processing Unit (CPU)-based FDTD simulator.

Cross-Linkable Gelatins with Superior Mechanical Properties Through Carboxylic Acid Modification: Increasing the Two-Photon Polymerization Potential

The present work reports on the development of photo-cross-linkable gelatins sufficiently versatile to overcome current biopolymer two-photon polymerization (2PP) processing limitations. To this end, both the primary amines as well as the carboxylic acids of gelatin type B were functionalized with photo-cross-linkable moieties (up to 1 mmol/g) resulting in superior and tunable mechanical properties (G′ from 5000 to 147000 Pa) enabling efficient 2PP processing. The materials were characterized in depth prior to and after photoinduced cross-linking using fully functionalized gelatin-methacrylamide (gel-MOD) as a benchmark to assess the effect of functionalization on the protein properties, cross-linking efficiency, and mechanical properties. In addition, preliminary experiments on hydrogel films indicated excellent in vitro biocompatibility (close to 100% viability) both in the presence of MC3T3 preosteoblasts and L929 fibroblasts. Moreover, 2PP processing of the novel derivative was superior in terms of applied laser power (≥40 vs ≥60 mW for gel-MOD at 100 mm/s) as well as post-production swelling (0–20% vs 75–100% for gel-MOD) compared to those of gel-MOD. The reported novel gelatin derivative (gel-MOD-AEMA) proves to be extremely suitable for direct laser writing as both superior mimicry of the applied computer-aided design (CAD) was obtained while maintaining the desired cellular interactivity of the biopolymer. It can be anticipated that the present work will also be applicable to alternative biopolymers mimicking the extracellular environment such as collagen, elastin, and glycosaminoglycans, thereby expanding current material-related processing limitations in the tissue engineering field.

Indirect Rapid Prototyping: Opening Up Unprecedented Opportunities in Scaffold Design and Applications

Over the past decades, solid freeform fabrication (SFF) has emerged as the main technology for the production of scaffolds for tissue engineering applications as a result of the architectural versatility. However, certain limitations have also arisen, primarily associated with the available, rather limited range of materials suitable for processing. To overcome these limitations, several research groups have been exploring novel methodologies through which a construct, generated via SFF, is applied as a sacrificial mould for production of the final construct. The technique combines the benefits of SFF techniques in terms of controlled, patient-specific design with a large freedom in material selection associated with conventional scaffold production techniques. Consequently, well-defined 3D scaffolds can be generated in a straightforward manner from previously difficult to print and even “unprintable” materials due to thermomechanical properties that do not match the often strict temperature and pressure requirements for direct rapid prototyping. These include several biomaterials, thermally degradable materials, ceramics and composites. Since it can be combined with conventional pore forming techniques, indirect rapid prototyping (iRP) enables the creation of a hierarchical porosity in the final scaffold with micropores inside the struts. Consequently, scaffolds and implants for applications in both soft and hard tissue regeneration have been reported. In this review, an overview of different iRP strategies and materials are presented from the first reports of the approach at the turn of the century until now.