Jyoti Rawre
All India Institute of Medical Sciences
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Featured researches published by Jyoti Rawre.
Indian Journal of Pathology & Microbiology | 2013
Prathyusha Kokkayil; Jyoti Rawre; Neena Malhotra; Benu Dhawan
Genital tuberculosis is a common cause of female infertility in India. But, it is important to screen for other agents like Chlamydia trachomatis and genital Mycoplasmas as well to avoid persistence of infection and its long-term sequelae. Timely diagnosis of these infections using nucleic acid amplification tests and institution of appropriate therapy will improve the conception rates in infertile women. We report a case of co-infection of Mycoplasma genitalium and Chlamydia trachomatis in an infertile female patient with genital tuberculosis. The infections were diagnosed using polymerase chain reaction, and the patient responded to a combination of antituberculosis therapy and 1 g single-dose Azithromycin.
Apmis | 2016
Jyoti Rawre; Benu Dhawan; Neena Malhotra; Vishnubhatla Sreenivas; Shobha Broor; Rama Chaudhry
To determine the prevalence and distribution of Chlamydia trachomatis genovars in patients with infertility by PCR‐RFLP and ompA gene sequencing. Prevalence of other etiological agents (viz., Ureaplasma spp. and Mycoplasma hominis) were also assessed. Endocervical swabs were collected from 477 women and urine was collected from 151 men attending the Infertility Clinic. The samples were screened for C. trachomatis by cryptic plasmid, ompA gene and nested ompA gene PCR. Genotyping was performed by PCR‐RFLP and sequencing. Samples were screened for Ureaplasma spp. and M. hominis. The prevalence of C. trachomatis in infertile women and their male partners were 15.7% (75 of 477) and 10.0% (15 of 151) respectively. Secondary infertility was significantly associated with chlamydial infection. Genovar E was the most prevalent followed by genovar D and F. Twenty‐four C. trachomatis strains were selected for ompA gene sequencing. No mixed infection was picked. Variability in ompA sequences was seen in 50.0%. Both PCR‐RFLP and ompA gene sequencing showed concordant results. High prevalence of C. trachomatis in infertile couples warrants routine screening for C. trachomatis infection in all infertile couples. Genotyping of the ompA gene of C. trachomatis may be a valuable tool in understanding the natural history of C. trachomatis infection.
Indian Journal of Medical Microbiology | 2013
Arnab Ghosh; Jyoti Rawre; Neena Khanna; Benu Dhawan
A 30-year-old human immunodeficiency virus (HIV)-1 infected woman presented with vaginal discharge and associated vulval irritation. The vaginal swabs tested positive for Ureaplasma parvum and Mycoplasma hominis by both culture and polymerase chain reaction (PCR). The specimen also tested positive for Chlamydia trachomatis deoxyribonucleic acid (DNA) by cryptic plasmid and omp1 gene PCR assays. The patient was successfully treated with azithromycin based on the antibiotic susceptibility testing results of U. parvum and M. hominis by microbroth dilution. Since sexually transmitted infections enhance the transmission of HIV, HIV-positive patients should be screened routinely for these pathogens.
Indian Journal of Medical Microbiology | 2017
Jyoti Rawre; Deepak Juyal; Benu Dhawan
Urogenital infection due to Chlamydia trachomatis (CT) is one of the most common bacterial sexually transmitted infections (STIs) and is a major public health problem worldwide. Molecular characterisation of CT is important for understanding the pathophysiological mechanisms of chlamydial disease and its transmission dynamics in sexual networks. Traditionally, strain typing of CT was based on serotyping methods characterising the major outer membrane protein (MOMP). With the advent of polymerase chain reaction and sequencing the era of molecular typing began. Molecular characterization of CT strains is based on sequence analysis of ompA gene encoding MOMP. However, in due course of time, improvements were made to enhance the discriminatory power of sequencing and quality of epidemiological information. New high-resolution genotyping methods using multiple loci such as multilocus sequence typing (MLST) and multiple loci variable number of tandem repeats (MLVA) were developed but were unable to differentiate mixed infections (MIs). The development of DNA-hybridisation methods emerged as a major breakthrough in detecting MIs. Although MLST and MLVA are more discriminative than other genotyping methods, they are laborious and expensive. DNA microarray technique is an affordable alternative for genotyping. Since recombination is widespread in the CT genome, ompA is not a reliable marker for phylogenetic studies; hence, whole genome sequencing may provide maximum phylogenetic resolution of CT strains. A descriptive review is provided of the various molecular CT typing methods. The vital information gained can be used for formulating screening programmes, targeted prevention and optimising therapeutic measures aiming to reduce disease transmission.
Journal of Medical Microbiology | 2016
Megha Brijwal; Benu Dhawan; Jyoti Rawre; Sujeesh Sebastian; Arti Kapil
Linezolid, an oxazolidinone drug available in both parenteral and oral formulations, has emerged as a novel alternative to vancomycin and other second-generation drugs for treatment of infections from Gram-positive cocci (Gu et al., 2013). Mutation at domain V of 23S rRNA can result in modification of the ribososmal peptidyl transferase centre region and resistance in isolates. A naturally occurring resistance gene cfr, encoding a cfr methyltransferase, has also been reported in clinical isolates. Moreover, resistance has also been associated with mutations in the genes for the ribosomal proteins L3 and L4, which interact closely with the linezolid binding site in the peptidyl transferase centre region (Long & Vester, 2012). Linezolid resistance in clinical staphylococcus isolates is increasingly being reported worldwide (Jones et al., 2009). Here, we describe four linezolidresistant clinical isolates of Staphylococcus haemolyticus with a dual mechanism of resistance.
Indian Journal of Sexually Transmitted Diseases | 2015
Karnika Saigal; Jyoti Rawre; Neena Khanna; Benu Dhawan
2. Tripathy S, Pereira M, Tripathy SP. HIV testing in India. Clin Lab Med 2012;32:175‐91. 3. National AIDS Control Organization (NACO). Operational Guidelines for Integrated Counselling and Testing Centres. New Delhi (India): National AIDS Control Organization, Ministry of Health and Family Welfare, Government of India; 2007. 4. Chaudhary R, Das SS, Ojha S, Khetan D, Sonker A. The external quality assessment scheme: Five years experience as a participating laboratory. Asian J Transfus Sci 2010;4:28‐30. Access this article online Quick Response Code: Website:
Indian Journal of Medical Microbiology | 2018
Jyoti Rawre; Sonu Agrawal; Benu Dhawan
Extragenital infections can occur concurrently with simultaneous urogenital infections. Extragenital sites are believed to serve as hidden reservoirs and play a critical role in their transmission. The etiological relationship of the most widespread Sexually transmitted diseases (STD) pathogen to reproductive tract has long been established, but the distribution to extragenital sites appears to be infrequent and its correlation with the sexual practice still requires to be investigated. Optimal-screening strategies for extragenital infections are largely unknown. However, there is a lack of data on clinical outcomes and optimal treatment regimens for rectal and pharyngeal extragenital infections. Further studies are needed in settings other than reproductive health and STD clinics, especially in primary care clinics and resource-limited settings.
Indian Journal of Pathology & Microbiology | 2016
Karnika Saigal; Benu Dhawan; Jyoti Rawre; Neena Khanna; Rama Chaudhry
Limited data are available on the prevalence of genital mycoplasmas and Chlamydia trachomatis (CT) among Indian patients with genital tract infections. The objectives of the study were to determine the prevalence of Ureaplasma urealyticum (UU), Mycoplasma hominis (MH), Mycoplasma genitalium (MG), and CT in patients with genital tract infections. The antimicrobial susceptibilities of UU and MH were also assessed. Endocervical swabs/urethral swabs and first void urine samples of patients (n = 164) were collected. UU and MH were detected by culture and multiplex polymerase chain reaction (PCR). MG and CT were identified by PCR. Ureaplasma isolates were further biotyped and serotyped. Antimicrobial susceptibility was done by microbroth dilution method. UU, MH, MG, and CT were detected in 15.2%, 5.4%, 1.2%, and 6% patients, respectively. Ureaplasma parvum serovar 3/14 was the most prevalent. All isolates of UU and MH were uniformly susceptible to doxycycline and josamycin. Routine screening for these pathogens and antimicrobial susceptibility testing is warranted to prevent sequel of infections and formulate treatment guidelines.
Indian Journal of Dermatology, Venereology and Leprology | 2016
Jyoti Rawre; Benu Dhawan; Karnika Saigal; Neena Khanna
We report a case of Chlamydia trachomatis serovar G urogenital tract infection in a 33-year-old human immunodeficiency virus-1 (HIV-1) seropositive Indian bisexual male. This case highlights the emergence of a new serovar in India. The patient was tested positive for C. trachomatis by both cryptic plasmid and omp A gene polymerase chain reaction (PCR). On further characterization using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) and omp A gene sequencing, the strain was found to be C. trachomatis serovar G. His spouse was also found to be infected with C. trachomatis serovar G. Phylogenetic analysis was performed on the clinical isolates obtained from both partners and were found to be identical to the isolates available in GenBank. The sexual network could not be traced further. Detection of a new genotype suggests importation of a new strain into the population probably by sexual contact with a person from a geographical area where the strain is common. Identifying circulating genotypes in the community can assist in developing strategies for improved sexually transmitted disease control.
Indian Journal of Medical Research | 2012
Benu Dhawan; Neena Malhotra; Vishnubhatla Sreenivas; Jyoti Rawre; Neena Khanna; Rama Chaudhry; Suneeta Mittal