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Dive into the research topics where Kamila Puppel is active.

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Featured researches published by Kamila Puppel.


Journal of the Science of Food and Agriculture | 2012

Differences in whey protein content between cow's milk collected in late pasture and early indoor feeding season from conventional and organic farms in Poland

Beata Kuczyńska; Kamila Puppel; Marcin Gołȩbiewski; Ewa Metera; Tomasz Sakowski; Krzysztof Słoniewski

BACKGROUND The aim of the study was to investigate bioactive whey protein concentrations in cows milk collected in late pasture (LP) and early indoor feeding (EIF) season from conventional and organic farms in Poland. RESULTS Results showed that in the LP somatic cell count (SCC) was higher under organic farming conditions. However, percentages of protein and fat were higher under conventional farming conditions. In EIF, milk from conventional dairy farms had a higher percentage of fat and lactose and a lower concentration of protein and SCC in comparison to milk from organic farms. Organic milk in LP had higher concentrations of beneficial whey proteins than conventional milk, including β-lactoglobulin (β-Lg, 4.12 vs. 2.68 g L⁻¹), lactoferrin (Lf, 334.99 vs. 188.02 mg L⁻¹), and lysozyme (Lz, 15.68 vs. 12.56 µg L⁻¹). However, conventional milk in EIF had higher concentrations of bovine serum albumin (146.47 vs. 118.65 mg L⁻¹), Lf (49 vs. 185.27 mg L⁻¹), and Lz (16.63 vs. 13.22 µg L⁻¹). CONCLUSIONS The results show significant differences in the investigated parameters between organic milk and milk from conventional system during EIF and LP. Moreover, extending the pasture season during EIF in organic farms decreases concentration of bioactive compounds of milk.


Journal of the Science of Food and Agriculture | 2012

The age of cows as a factor shaping the antioxidant level during a nutritional experiment with fish oil and linseed supplementation for increasing the antioxidant value of milk

Kamila Puppel; Teresa Nałȩcz‐Tarwacka; Beata Kuczyńska; Marcin Gołȩbiewski; Marta Kordyasz; H. Grodzki

BACKGROUND So far, in research studies, the age of cows has not been considered as a factor that may influence the changes in the content of milk ingredients with antioxidant properties modified by the feed supplementation. The aim of this study was to determine the influence of supplementation on the content of ingredients having antioxidant properties and to determine the influence of the age of cows taking part in the experiment on these changes. The experiment was conducted using 20 Polish Holstein Friesian cows, 10 primiparous and 10 multiparous. The combined supplementation of fish oil and linseed constituted the experimental factor. RESULTS The milk of primiparous cows after 21 days of supplementation was characterised by a higher content of C18:1 trans-11, C18:2 cis-9, trans-11, α-retinol, α-tocopherol and β-lactoglobulin compared to the milk of multiparous cows, in which a higher level of lactoferrin, C20:5 and β-carotene was recorded. In both groups an increase in the total antioxidant status was noted (a higher level in the milk of primiparous cows). CONCLUSIONS Modification of the diet of cows with fish oil and linseed significantly influenced antioxidant properties of their milk; however, the response of multiparous and primaparous cows was noticeably different to the supplement introduced.


Journal of the Science of Food and Agriculture | 2015

The etiology of oxidative stress in the various species of animals, a review

Kamila Puppel; Aleksandra Kapusta; Beata Kuczyńska

Oxidative stress is the consequence of an imbalance of pro-oxidants and antioxidants leading to cell damage and tissue injury. The exhaustion of antioxidant systems is one of the reasons for the occurrence of oxidative stress, which results in avalanche production of reactive oxygen species (ROS) or free radicals. High oxidative stress is common in organs and tissues with high metabolic and energy demands, including skeletal and heart muscle, liver and blood cells. Stress arises in animals in response to unavoidable or adverse environmental conditions. In the external environment, which affects the body of the cow, there are four main groups of stressors: physical, chemical, biological and psychological. Physical stressors include fluctuations in ambient temperature as well as mechanical injuries. High ambient temperature is one of the factors affecting the productivity of cows. Biological stressors are conditioned by errors and irregularities in habits. Both of these phenomena have an adverse impact on both the resistance of animals and fertility and are the etiological agent of oxidative stress. Various mechanisms may be responsible for metal-induced oxidative stress: direct or indirect generation of ROS, depletion of glutathione and inhibition of antioxidant enzymes are well known for all redox-active and redox-inactive metals.


Journal of the Science of Food and Agriculture | 2016

Effect of supplementation of cows diet with linseed and fish oil and different variants of β-lactoglobulin on fatty acid composition and antioxidant capacity of milk

Kamila Puppel; Beata Kuczyńska; Teresa Nałęcz-Tarwacka; M Golebiewski; Tomasz Sakowski; Aleksandra Kapusta; Arkadiusz Budziński; Marek Balcerak

BACKGROUND The aim of this study was to determine the influence of polymorphic variants of β-lactoglobulin in cows supplemented with linseed and fish oil on the fatty acid composition and antioxidant capacity of milk. From the herd of 320 Polish Holstein Friesian cows three groups of cows were selected according to the variants of β-LG (β-LGAA, β-LGBB, β-LGAB). During the first 7 days (the initial period) all the cows were fed the same total mixed ration (TMR) diet. From day 8 to 28,150 g fish oil and 250 g linseed (FOL) was added to the TMR diet of each cow. RESULTS The results showed that the diet supplemented with FOL was effective in reducing atherogenic and thrombogenic indices. Introducing supplementation improved the antioxidant capacity: higher concentration of C18:2cis-9 trans-11, C20:5 n-3, C22:6 n-3, bioactive whey proteins and vitamin soluble in fat has been recorded. The results showed that β-LGAA was associated with lower levels of atherogenic and thrombogenic indices and higher concentration of C22:5 n-6, phospholipids and β-carotene. β-LGBB favours a higher content of C18:1trans-11, C18:2cis-9 trans-11 and lactoferrin. β-LGAB was associated with higher concentrations of C20:5 n-3, Lysozyme, α-retinol, α-tocopherol and total antioxidant status. CONCLUSION Modification of the diet of cows with fish oil and linseed significantly influenced fatty acid composition and antioxidant properties of milk. The effect of β-LG phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a β-LG phenotype × diet interaction.


Journal of the Science of Food and Agriculture | 2013

Influence of linseed variety on fatty acid profile in cow's milk

Kamila Puppel; Beata Kuczyńska; Teresa Nałęcz-Tarwacka; H. Grodzki

BACKGROUND The aim of this study was to examine the influence of linseed variety on the concentration of cows milk constituents, particularly fatty acids. The experiment was conducted on 30 Polish Holstein Friesian cows whose diet was supplemented with two varieties of crude linseed, Opal and Szafir. RESULTS After 21 days of linseed supplementation, the Szafir variety proved to be a better supplement than the Opal variety, particularly in relation to the concentration of saturated fatty acids, C20:5 (69.2% higher) and C22:6 (147.1% higher) and also because of improved chemical composition of the milk (19.7% higher fat, 2.9% higher protein and 39.9% higher casein content). CONCLUSION Linseed variety significantly influenced the lipid fraction level and the basic chemical composition of cows milk. Linseed variety should therefore be taken into consideration in subsequent experiments in addition to the quantity and physical form of linseed. The results showed that the use of a diet supplemented with linseed, especially the Szafir variety, was effective in reducing saturation, atherogenic and thrombogenic indices, yielding benefits for consumers by improving the nutritional quality of cows milk.


Journal of the Science of Food and Agriculture | 2012

Relationships between physiological indicators in blood, and their yield, as well as chemical composition of milk obtained from organic dairy cows

Tomasz Sakowski; Beata Kuczyńska; Kamila Puppel; Ewa Metera; Krzysztof Słoniewski; Jerzy Barszczewski

BACKGROUND The occurrence of metabolic overload in cows maintained in organic herds is very likely, because organic production promotes feeding with high roughage diets and discourages supplementation with concentrates supplementation. It has the potential to negatively affect milk quality. The aim of this study was to determine the influence of physiological indicators in blood, reflecting the energy metabolism and liver status of dairy cows kept in organic herds, on the content of bioactive components in milk and the influence of lactation stage and feeding season on changes in milk components. RESULTS A significant positive correlation was found between β-hydroxybutyric acid and the daily milk yield of lactating cows. β-Hydroxybutyric acid was negatively correlated with lactation phase and polyunsaturated fatty acids as well as saturated fatty acids content in milk. A significant negative correlation was observed between average daily milk yield and alanine aminotransferase concentration in blood. A low level of production in organic farms had a positive impact on the content of conjugated linoleic acid (20% increase), vitamin A (21% increase) and vitamin E (11% increase). CONCLUSION The study has shown significant correlations between metabolic profiles and milk yields, as well as milk composition. In addition, there is evidence that the intensity of the production system significantly influences the metabolic profile and chemical composition of cows milk. It could even be concluded that the ecological system affects not only the quality of milk, but also the health of cows.


PLOS ONE | 2015

Screening for the Most Suitable Reference Genes for Gene Expression Studies in Equine Milk Somatic Cells.

Jakub Cieslak; Mariusz Mackowski; Grażyna Czyżak-Runowska; Jacek Wójtowski; Kamila Puppel; Beata Kuczyńska; Piotr Pawlak

Apart from the well-known role of somatic cell count as a parameter reflecting the inflammatory status of the mammary gland, the composition of cells isolated from milk is considered as a valuable material for gene expression studies in mammals. Due to its unique composition, in recent years an increasing interest in mares milk consumption has been observed. Thus, investigating the genetic background of horse’s milk variability presents and interesting study model. Relying on 39 milk samples collected from mares representing three breeds (Polish Primitive Horse, Polish Cold-blooded Horse, Polish Warmblood Horse) we aimed to investigate the utility of equine milk somatic cells as a source of mRNA and to screen the best reference genes for RT-qPCR using geNorm and NormFinder algorithms. The results showed that despite relatively low somatic cell counts in mares milk, the amount and the quality of the extracted RNA are sufficient for gene expression studies. The analysis of the utility of 7 potential reference genes for RT-qPCR experiments for the normalization of equine milk somatic cells revealed some differences between the outcomes of the applied algorithms, although in both cases the KRT8 and TOP2B genes were pointed as the most stable. Analysis by geNorm showed that the combination of 4 reference genes (ACTB, GAPDH, TOP2B and KRT8) is required for apropriate RT-qPCR experiments normalization, whereas NormFinder algorithm pointed the combination of KRT8 and RPS9 genes as the most suitable. The trial study of the relative transcript abundance of the beta-casein gene with the use of various types and numbers of internal control genes confirmed once again that the selection of proper reference gene combinations is crucial for the final results of each real-time PCR experiment.


Journal of the Science of Food and Agriculture | 2014

Effect of fish oil and linseed supplementation on the protein composition of milk from cows with different β-lactoglobulin phenotypes.

Kamila Puppel; Beata Kuczyńska; Teresa Nałęcz-Tarwacka; Tomasz Sakowski; M Golebiewski; Małgorzata Kunowska-Slósarz; Arkadiusz Budziński; H. Grodzki

BACKGROUND The objective of this study was to determine the impact of fish oil and linseed (FOL) supplements on the protein fraction levels of milk from cows with different phenotypes of β-lactoglobulin. RESULTS After 21 days of supplementation the study showed significantly higher concentrations of whey proteins, especially lysozyme (144% increase) and lactoferrin (45.5% increase), compared with milk from control cows (total mixed ration with no supplemented FOL). A reverse trend was demonstrated for casein, casein index and casein number (lower level). The most favourable change (higher level), in terms of lactoferrin, α-lactalbumin and bovine serum albumin contents in milk, was recorded in cows with the BB variant of β-lactoglobulin. The highest level of lysozyme was recorded in the milk of cows with the AB variant of β-lactoglobulin. CONCLUSION The combined supplementation of fish oil and linseed had a positive impact on whey proteins in cows milk. In addition, the phenotype of β-lactoglobulin also played a role in milk protein composition. There is therefore a clear indication that nutritional experiments should take into account not only food supplements but also the genetic variants of β-lactoglobulin.


Journal of Dairy Science | 2016

Characterization of equine CSN1S2 variants considering genetics, transcriptomics, and proteomics

Jakub Cieslak; Piotr Pawlak; Lukasz Wodas; Alicja Borowska; Anna Stachowiak; Kamila Puppel; Beata Kuczyńska; Magdalena Luczak; Lukasz Marczak; Mariusz Mackowski

Currently, research interest is increasing in horse milk composition and its effect on human health. Despite previously published studies describing the presence of intra- and interbreed variability of equine milk components, no investigations have focused on the genetic background of this variation. Among horse caseins and the genes encoding them, least is known about the structure and expression of the α-S2 casein gene, CSN1S2. Herein, based on direct sequencing of the equine CSN1S2 coding sequence, we describe the presence of 51-bp insertion-deletion (in/del) polymorphism, which significantly changes the protein sequence (lack or presence of 17-amino acid serine-rich peptide). Bioinformatic analysis revealed that the observed in/del polymorphism spanned exactly 2 exons; therefore, we hypothesized that we were observing different CSN1S2 splicing isoforms. However, further investigation indicated that the detected sequence variation was caused by a large (1.3-kb) deletion in the genomic DNA. We found that the polymorphic forms (A, longer; B, shorter; KP658381 and KP658382 GenBank records, respectively) were unevenly distributed among different horse breeds (the highest frequency of variant B was observed in coldblood horses and Haflingers). We propose that the analyzed polymorphism is associated with CSN1S2 expression level (the highest expression was recorded for individuals carrying the BB genotype), which was much more pronounced for milk CSN1S2 protein content than for relative transcript abundance (measured in milk somatic cells). Our results provide insight into the equine CSN1S2 structure and lay a foundation for further functional analyses regarding, for example, allergenicity or physiochemical properties of the observed CSN1S2 variants.


PLOS ONE | 2018

Relationship between the degree of antioxidant protection and the level of malondialdehyde in high-performance Polish Holstein-Friesian cows in peak of lactation

Aleksandra Kapusta; Beata Kuczyńska; Kamila Puppel

Lipid peroxidation can be described as a process under which free radicals attack carbon double bonds of omega-3 and omega-6 fatty acids. Whereas the end products of this process are reactive aldehydes, such as malondialdehyde (MDA). Lipid peroxidation leads to adverse changes in the nutritional value of milk; therefore, higher degree of antioxidant protection (DAP) ensures higher stability of dairy products by effecting their high antioxidative potential. Therefore, the purpose of this study was to demonstrate the relationship between the DAP and the level of MDA in high-performance Polish Holstein-Friesian cows in peak of lactation. Sixty-three Polish Holstein-Friesian cows were selected to the experiment according to: parity (all in the 2nd lactation), phase of lactation (peak of lactation), cytological quality of milk (somatic cell count < 150 thousand/ml) and without diagnosed metabolic diseases. The data obtained were analyzed statistically by two–way ANOVA, and Tukey’s post-hoc test. After analysis of performance the cows were divided into 3 groups (twenty one cows in each group) based on milk yield and MDA concentration. The study revealed a significant effect of the lactation performance of cows on MDA levels in milk (P ≤ 0.01). The highest concentration of MDA (61.137 nM/mL) was shown in milk of cows yielding between 50.00 and 55.80 kg/day. The highest concentration of fat was found in milk in which the MDA level ranged from 48 to 86 nM/mL. Whereas, the inverse relationship was demonstrated in case of protein concentration. The highest level of protein was found in cows with MDA levels in the range of 18–28 nM/mL (P ≤ 0.01). The lowest MDA level (in the range of 18–28 nM/mL) was associated with the highest concentration of vitamin E, β-carotene, total antioxidant status (TAS) and DAP, measured in both milk and plasma. The obtained results show that lipid peroxidation leads to adverse changes in the nutritional value of milk; the highest DAP (7.89 x 10−3) was found in the cows with the lowest MDA concentration in milk.

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Beata Kuczyńska

Warsaw University of Life Sciences

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M Golebiewski

Warsaw University of Life Sciences

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Teresa Nałęcz-Tarwacka

Warsaw University of Life Sciences

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H. Grodzki

Warsaw University of Life Sciences

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Aleksandra Kapusta

Warsaw University of Life Sciences

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Arkadiusz Budziński

Warsaw University of Life Sciences

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A Wojcik

Warsaw University of Life Sciences

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Jakub Cieslak

University of Life Sciences in Poznań

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